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bcl-2 gene enables rescue from in vitro myelosuppression (bone marrow cell death) induced by chemotherapy.
Recent studies have shown that the use of cytokines such as granulocyte colony-stimulating factor (G-CSF) to ameliorate chemotherapy-induced myelosuppression may enhance the viability of tumour cells with functional receptors for these cytokines. In this study, therefore, we used murine bone marrow...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
1994
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033346/ https://www.ncbi.nlm.nih.gov/pubmed/8080725 |
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author | Kondo, S. Yin, D. Takeuchi, J. Morimura, T. Oda, Y. Kikuchi, H. |
author_facet | Kondo, S. Yin, D. Takeuchi, J. Morimura, T. Oda, Y. Kikuchi, H. |
author_sort | Kondo, S. |
collection | PubMed |
description | Recent studies have shown that the use of cytokines such as granulocyte colony-stimulating factor (G-CSF) to ameliorate chemotherapy-induced myelosuppression may enhance the viability of tumour cells with functional receptors for these cytokines. In this study, therefore, we used murine bone marrow (BM) cells in an in vitro model in an attempt to determine whether topoisomerase inhibitors (camptothecin, etoposide and doxorubicin) induce myelosuppression (BM cell death) and whether novel treatments other than the administration of G-CSF can be used for rescue from myelosuppression. DNA fragmentation assay, ultrastructural analysis and cell cycle analysis demonstrated that these chemotherapeutic agents induced apoptosis in BM cells. We demonstrated in addition that enforced expression of the bcl-2 gene in BM cells by MPZenNeo (bcl-2) retroviral gene transfer increased resistance to the apoptosis induced by these agents. These findings suggest the possibility that enforced expression of the bcl-2 gene in BM cells using gene transfer techniques may enable rescue from chemotherapy-induced myelosuppression. IMAGES: |
format | Text |
id | pubmed-2033346 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 1994 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-20333462009-09-10 bcl-2 gene enables rescue from in vitro myelosuppression (bone marrow cell death) induced by chemotherapy. Kondo, S. Yin, D. Takeuchi, J. Morimura, T. Oda, Y. Kikuchi, H. Br J Cancer Research Article Recent studies have shown that the use of cytokines such as granulocyte colony-stimulating factor (G-CSF) to ameliorate chemotherapy-induced myelosuppression may enhance the viability of tumour cells with functional receptors for these cytokines. In this study, therefore, we used murine bone marrow (BM) cells in an in vitro model in an attempt to determine whether topoisomerase inhibitors (camptothecin, etoposide and doxorubicin) induce myelosuppression (BM cell death) and whether novel treatments other than the administration of G-CSF can be used for rescue from myelosuppression. DNA fragmentation assay, ultrastructural analysis and cell cycle analysis demonstrated that these chemotherapeutic agents induced apoptosis in BM cells. We demonstrated in addition that enforced expression of the bcl-2 gene in BM cells by MPZenNeo (bcl-2) retroviral gene transfer increased resistance to the apoptosis induced by these agents. These findings suggest the possibility that enforced expression of the bcl-2 gene in BM cells using gene transfer techniques may enable rescue from chemotherapy-induced myelosuppression. IMAGES: Nature Publishing Group 1994-09 /pmc/articles/PMC2033346/ /pubmed/8080725 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Research Article Kondo, S. Yin, D. Takeuchi, J. Morimura, T. Oda, Y. Kikuchi, H. bcl-2 gene enables rescue from in vitro myelosuppression (bone marrow cell death) induced by chemotherapy. |
title | bcl-2 gene enables rescue from in vitro myelosuppression (bone marrow cell death) induced by chemotherapy. |
title_full | bcl-2 gene enables rescue from in vitro myelosuppression (bone marrow cell death) induced by chemotherapy. |
title_fullStr | bcl-2 gene enables rescue from in vitro myelosuppression (bone marrow cell death) induced by chemotherapy. |
title_full_unstemmed | bcl-2 gene enables rescue from in vitro myelosuppression (bone marrow cell death) induced by chemotherapy. |
title_short | bcl-2 gene enables rescue from in vitro myelosuppression (bone marrow cell death) induced by chemotherapy. |
title_sort | bcl-2 gene enables rescue from in vitro myelosuppression (bone marrow cell death) induced by chemotherapy. |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033346/ https://www.ncbi.nlm.nih.gov/pubmed/8080725 |
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