Comparison of p53 gene mutation and protein overexpression in colorectal carcinomas.

Immunocytochemistry (ICC) has been used routinely to stain for p53 overexpression in a range of human tumours. The underlying assumption has been that positive staining indicates a mutation in the p53 coding sequence. Recently, however, discordancy has been observed and the accuracy of ICC as a mark...

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Autores principales: Dix, B., Robbins, P., Carrello, S., House, A., Iacopetta, B.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1994
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033416/
https://www.ncbi.nlm.nih.gov/pubmed/7917901
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author Dix, B.
Robbins, P.
Carrello, S.
House, A.
Iacopetta, B.
author_facet Dix, B.
Robbins, P.
Carrello, S.
House, A.
Iacopetta, B.
author_sort Dix, B.
collection PubMed
description Immunocytochemistry (ICC) has been used routinely to stain for p53 overexpression in a range of human tumours. The underlying assumption has been that positive staining indicates a mutation in the p53 coding sequence. Recently, however, discordancy has been observed and the accuracy of ICC as a marker of p53 gene mutation has been questioned. In this study of 109 colorectal adenocarcinomas, we compared ICC staining with p53 gene mutations detected by single-strand conformation polymorphism (SSCP) analysis. Concordancy between the two techniques was found in 69% of tumours. ICC-positive/SSCP-negative cases accounted for 20% of tumours and ICC-negative/SSCP-positive cases for the remaining 11%. These results caution against the assumption that p53 protein overexpression is always associated with a gene mutation. Epigenetic phenomena may account for a significant proportion of ICC-positive tumours. IMAGES:
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spelling pubmed-20334162009-09-10 Comparison of p53 gene mutation and protein overexpression in colorectal carcinomas. Dix, B. Robbins, P. Carrello, S. House, A. Iacopetta, B. Br J Cancer Research Article Immunocytochemistry (ICC) has been used routinely to stain for p53 overexpression in a range of human tumours. The underlying assumption has been that positive staining indicates a mutation in the p53 coding sequence. Recently, however, discordancy has been observed and the accuracy of ICC as a marker of p53 gene mutation has been questioned. In this study of 109 colorectal adenocarcinomas, we compared ICC staining with p53 gene mutations detected by single-strand conformation polymorphism (SSCP) analysis. Concordancy between the two techniques was found in 69% of tumours. ICC-positive/SSCP-negative cases accounted for 20% of tumours and ICC-negative/SSCP-positive cases for the remaining 11%. These results caution against the assumption that p53 protein overexpression is always associated with a gene mutation. Epigenetic phenomena may account for a significant proportion of ICC-positive tumours. IMAGES: Nature Publishing Group 1994-10 /pmc/articles/PMC2033416/ /pubmed/7917901 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Dix, B.
Robbins, P.
Carrello, S.
House, A.
Iacopetta, B.
Comparison of p53 gene mutation and protein overexpression in colorectal carcinomas.
title Comparison of p53 gene mutation and protein overexpression in colorectal carcinomas.
title_full Comparison of p53 gene mutation and protein overexpression in colorectal carcinomas.
title_fullStr Comparison of p53 gene mutation and protein overexpression in colorectal carcinomas.
title_full_unstemmed Comparison of p53 gene mutation and protein overexpression in colorectal carcinomas.
title_short Comparison of p53 gene mutation and protein overexpression in colorectal carcinomas.
title_sort comparison of p53 gene mutation and protein overexpression in colorectal carcinomas.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033416/
https://www.ncbi.nlm.nih.gov/pubmed/7917901
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