The use of genetic marking to assess the interaction of sensitive and multidrug-resistant cells in mixed culture.

The interaction of normal (CHO-K1) and multidrug-resistant (Adrr) Chinese hamster ovary cells was examined in mixed monolayer and spheroid culture. In order to assess the individual response of the two cell types in mixed culture, CHO-K1 was genetically marked by transfection with a bacterial beta-g...

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Detalles Bibliográficos
Autores principales: Bradley, C., Pitts, J.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1994
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033542/
https://www.ncbi.nlm.nih.gov/pubmed/7947083
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author Bradley, C.
Pitts, J.
author_facet Bradley, C.
Pitts, J.
author_sort Bradley, C.
collection PubMed
description The interaction of normal (CHO-K1) and multidrug-resistant (Adrr) Chinese hamster ovary cells was examined in mixed monolayer and spheroid culture. In order to assess the individual response of the two cell types in mixed culture, CHO-K1 was genetically marked by transfection with a bacterial beta-galactosidase gene. The enzyme product can be detected histochemically and allows identification of the marked cell line, designated CHO-K1-BG. Following administration of doxorubicin or mitozantrone, there was a large difference in the clonogenic survival of CHO-K1-BG and Adrr, whereas the overall survival of a 50:50 mixture of the two cell lines had intermediate values. When the survival of marked and unmarked colonies from mixed culture was assessed separately, there was no detectable alteration in chemosensitivity. We have found no evidence for interaction of sensitive and multidrug-resistant cells in this system.
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spelling pubmed-20335422009-09-10 The use of genetic marking to assess the interaction of sensitive and multidrug-resistant cells in mixed culture. Bradley, C. Pitts, J. Br J Cancer Research Article The interaction of normal (CHO-K1) and multidrug-resistant (Adrr) Chinese hamster ovary cells was examined in mixed monolayer and spheroid culture. In order to assess the individual response of the two cell types in mixed culture, CHO-K1 was genetically marked by transfection with a bacterial beta-galactosidase gene. The enzyme product can be detected histochemically and allows identification of the marked cell line, designated CHO-K1-BG. Following administration of doxorubicin or mitozantrone, there was a large difference in the clonogenic survival of CHO-K1-BG and Adrr, whereas the overall survival of a 50:50 mixture of the two cell lines had intermediate values. When the survival of marked and unmarked colonies from mixed culture was assessed separately, there was no detectable alteration in chemosensitivity. We have found no evidence for interaction of sensitive and multidrug-resistant cells in this system. Nature Publishing Group 1994-11 /pmc/articles/PMC2033542/ /pubmed/7947083 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Bradley, C.
Pitts, J.
The use of genetic marking to assess the interaction of sensitive and multidrug-resistant cells in mixed culture.
title The use of genetic marking to assess the interaction of sensitive and multidrug-resistant cells in mixed culture.
title_full The use of genetic marking to assess the interaction of sensitive and multidrug-resistant cells in mixed culture.
title_fullStr The use of genetic marking to assess the interaction of sensitive and multidrug-resistant cells in mixed culture.
title_full_unstemmed The use of genetic marking to assess the interaction of sensitive and multidrug-resistant cells in mixed culture.
title_short The use of genetic marking to assess the interaction of sensitive and multidrug-resistant cells in mixed culture.
title_sort use of genetic marking to assess the interaction of sensitive and multidrug-resistant cells in mixed culture.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033542/
https://www.ncbi.nlm.nih.gov/pubmed/7947083
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