Human papillomavirus type 18 DNA and E6-E7 mRNA are detected in squamous cell carcinoma and adenocarcinoma of the lung.

To provide an accurate evaluation of the association of human papillomavirus (HPV) with lung cancer, 36 cases of lung cancer were analysed for HPV DNAs by polymerase chain reaction (PCR) with dot-blot and Southern blot analyses, and for the transcripts from the E6-E7 transforming region by in situ h...

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Autores principales: Kinoshita, I., Dosaka-Akita, H., Shindoh, M., Fujino, M., Akie, K., Kato, M., Fujinaga, K., Kawakami, Y.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1995
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033613/
https://www.ncbi.nlm.nih.gov/pubmed/7841051
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author Kinoshita, I.
Dosaka-Akita, H.
Shindoh, M.
Fujino, M.
Akie, K.
Kato, M.
Fujinaga, K.
Kawakami, Y.
author_facet Kinoshita, I.
Dosaka-Akita, H.
Shindoh, M.
Fujino, M.
Akie, K.
Kato, M.
Fujinaga, K.
Kawakami, Y.
author_sort Kinoshita, I.
collection PubMed
description To provide an accurate evaluation of the association of human papillomavirus (HPV) with lung cancer, 36 cases of lung cancer were analysed for HPV DNAs by polymerase chain reaction (PCR) with dot-blot and Southern blot analyses, and for the transcripts from the E6-E7 transforming region by in situ hybridisation (ISH). HPV-18 DNA was detected in three (8%) of 36 specimens; histologically, in one (10%) of 10 squamous cell carcinomas and two (9%) of 22 adenocarcinomas. Neither HPV-16 nor -33 DNA was detected in any cases examined. Expression of E6-E7 mRNA was confirmed in the cases which contained, HPV-18 DNA. HPV-18 may play an important role in the development and progression of cancer in some cases of both squamous cell carcinoma and adenocarcinoma of the lung. IMAGES:
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spelling pubmed-20336132009-09-10 Human papillomavirus type 18 DNA and E6-E7 mRNA are detected in squamous cell carcinoma and adenocarcinoma of the lung. Kinoshita, I. Dosaka-Akita, H. Shindoh, M. Fujino, M. Akie, K. Kato, M. Fujinaga, K. Kawakami, Y. Br J Cancer Research Article To provide an accurate evaluation of the association of human papillomavirus (HPV) with lung cancer, 36 cases of lung cancer were analysed for HPV DNAs by polymerase chain reaction (PCR) with dot-blot and Southern blot analyses, and for the transcripts from the E6-E7 transforming region by in situ hybridisation (ISH). HPV-18 DNA was detected in three (8%) of 36 specimens; histologically, in one (10%) of 10 squamous cell carcinomas and two (9%) of 22 adenocarcinomas. Neither HPV-16 nor -33 DNA was detected in any cases examined. Expression of E6-E7 mRNA was confirmed in the cases which contained, HPV-18 DNA. HPV-18 may play an important role in the development and progression of cancer in some cases of both squamous cell carcinoma and adenocarcinoma of the lung. IMAGES: Nature Publishing Group 1995-02 /pmc/articles/PMC2033613/ /pubmed/7841051 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Kinoshita, I.
Dosaka-Akita, H.
Shindoh, M.
Fujino, M.
Akie, K.
Kato, M.
Fujinaga, K.
Kawakami, Y.
Human papillomavirus type 18 DNA and E6-E7 mRNA are detected in squamous cell carcinoma and adenocarcinoma of the lung.
title Human papillomavirus type 18 DNA and E6-E7 mRNA are detected in squamous cell carcinoma and adenocarcinoma of the lung.
title_full Human papillomavirus type 18 DNA and E6-E7 mRNA are detected in squamous cell carcinoma and adenocarcinoma of the lung.
title_fullStr Human papillomavirus type 18 DNA and E6-E7 mRNA are detected in squamous cell carcinoma and adenocarcinoma of the lung.
title_full_unstemmed Human papillomavirus type 18 DNA and E6-E7 mRNA are detected in squamous cell carcinoma and adenocarcinoma of the lung.
title_short Human papillomavirus type 18 DNA and E6-E7 mRNA are detected in squamous cell carcinoma and adenocarcinoma of the lung.
title_sort human papillomavirus type 18 dna and e6-e7 mrna are detected in squamous cell carcinoma and adenocarcinoma of the lung.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033613/
https://www.ncbi.nlm.nih.gov/pubmed/7841051
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