Expression of interleukin 10 in human melanoma.

The expression of interleukin 10 (IL-10) mRNA in human malignant melanoma was investigated by reverse transcriptase polymerase chain reaction analysis. Selective expression of IL-10 mRNA in tissues of primary melanomas and melanoma metastases was found in comparison with normal skin. In addition, st...

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Autores principales: Krüger-Krasagakes, S., Krasagakis, K., Garbe, C., Schmitt, E., Hüls, C., Blankenstein, T., Diamantstein, T.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1994
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033698/
https://www.ncbi.nlm.nih.gov/pubmed/7981073
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author Krüger-Krasagakes, S.
Krasagakis, K.
Garbe, C.
Schmitt, E.
Hüls, C.
Blankenstein, T.
Diamantstein, T.
author_facet Krüger-Krasagakes, S.
Krasagakis, K.
Garbe, C.
Schmitt, E.
Hüls, C.
Blankenstein, T.
Diamantstein, T.
author_sort Krüger-Krasagakes, S.
collection PubMed
description The expression of interleukin 10 (IL-10) mRNA in human malignant melanoma was investigated by reverse transcriptase polymerase chain reaction analysis. Selective expression of IL-10 mRNA in tissues of primary melanomas and melanoma metastases was found in comparison with normal skin. In addition, strong expression of IL-10 mRNA and of biologically active IL-10 was detected in 3 out of 13 melanoma cell lines. Normal melanocytes consistently expressed low levels of IL-10 mRNA but did not produce detectable IL-10 protein, nor did keratinocytes or fibroblasts. The production of biologically active IL-10 by melanoma cell lines suggests that IL-10 mRNA in melanoma lesions may derive at least in part from the tumour cells themselves. Tumour-infiltrating cells, however, could also be a source of IL-10 in melanoma tissues. The presence of IL-10 in melanoma lesions may contribute to the postulated 'paralysis' of an anti-melanoma immune response. IMAGES:
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spelling pubmed-20336982009-09-10 Expression of interleukin 10 in human melanoma. Krüger-Krasagakes, S. Krasagakis, K. Garbe, C. Schmitt, E. Hüls, C. Blankenstein, T. Diamantstein, T. Br J Cancer Research Article The expression of interleukin 10 (IL-10) mRNA in human malignant melanoma was investigated by reverse transcriptase polymerase chain reaction analysis. Selective expression of IL-10 mRNA in tissues of primary melanomas and melanoma metastases was found in comparison with normal skin. In addition, strong expression of IL-10 mRNA and of biologically active IL-10 was detected in 3 out of 13 melanoma cell lines. Normal melanocytes consistently expressed low levels of IL-10 mRNA but did not produce detectable IL-10 protein, nor did keratinocytes or fibroblasts. The production of biologically active IL-10 by melanoma cell lines suggests that IL-10 mRNA in melanoma lesions may derive at least in part from the tumour cells themselves. Tumour-infiltrating cells, however, could also be a source of IL-10 in melanoma tissues. The presence of IL-10 in melanoma lesions may contribute to the postulated 'paralysis' of an anti-melanoma immune response. IMAGES: Nature Publishing Group 1994-12 /pmc/articles/PMC2033698/ /pubmed/7981073 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Krüger-Krasagakes, S.
Krasagakis, K.
Garbe, C.
Schmitt, E.
Hüls, C.
Blankenstein, T.
Diamantstein, T.
Expression of interleukin 10 in human melanoma.
title Expression of interleukin 10 in human melanoma.
title_full Expression of interleukin 10 in human melanoma.
title_fullStr Expression of interleukin 10 in human melanoma.
title_full_unstemmed Expression of interleukin 10 in human melanoma.
title_short Expression of interleukin 10 in human melanoma.
title_sort expression of interleukin 10 in human melanoma.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033698/
https://www.ncbi.nlm.nih.gov/pubmed/7981073
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