Lack of a correlation between micronucleus formation and radiosensitivity in established and primary cultures of human tumours.

The radiation-induced genotoxic damage in three established cell lines and 15 primary cultures of human malignant melanoma and ovarian carcinoma showing different radiosensitivity was tested by the cytokinesis-block micronucleus assay. A dose-related increase in micronucleus frequency was observed i...

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Autores principales: Villa, R., Zaffaroni, N., Gornati, D., Costa, A., Silvestrini, R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1994
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033705/
https://www.ncbi.nlm.nih.gov/pubmed/7981062
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author Villa, R.
Zaffaroni, N.
Gornati, D.
Costa, A.
Silvestrini, R.
author_facet Villa, R.
Zaffaroni, N.
Gornati, D.
Costa, A.
Silvestrini, R.
author_sort Villa, R.
collection PubMed
description The radiation-induced genotoxic damage in three established cell lines and 15 primary cultures of human malignant melanoma and ovarian carcinoma showing different radiosensitivity was tested by the cytokinesis-block micronucleus assay. A dose-related increase in micronucleus frequency was observed in all the cell systems. The mean number of micronuclei per Gy of ionising radiation per binucleated cell was respectively 0.44 +/- 0.0075 and 0.43 +/- 0.04 for M14 and JR8 malignant melanoma cell lines and 0.19 +/- 0.013 for the A2780 ovarian cancer cell line. The number of micronuclei did not rank the cell lines in the same order of radiosensitivity as clonogenic cell survival, which showed a surviving fraction at 2 Gy of 0.38 +/- 0.02 for JR8, 0.34 +/- 0.05 for M14 and 0.22 +/- 0.007 for A2780. As regards primary tumour cultures, no correlation was observed between micronucleus induction and surviving fraction at 2 Gy. In conclusion, the discrepancy we observed between micronucleus formation and cell death raises doubts about the potential of the micronucleus assay as a preclinical means to predict radiosensitivity. IMAGES:
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spelling pubmed-20337052009-09-10 Lack of a correlation between micronucleus formation and radiosensitivity in established and primary cultures of human tumours. Villa, R. Zaffaroni, N. Gornati, D. Costa, A. Silvestrini, R. Br J Cancer Research Article The radiation-induced genotoxic damage in three established cell lines and 15 primary cultures of human malignant melanoma and ovarian carcinoma showing different radiosensitivity was tested by the cytokinesis-block micronucleus assay. A dose-related increase in micronucleus frequency was observed in all the cell systems. The mean number of micronuclei per Gy of ionising radiation per binucleated cell was respectively 0.44 +/- 0.0075 and 0.43 +/- 0.04 for M14 and JR8 malignant melanoma cell lines and 0.19 +/- 0.013 for the A2780 ovarian cancer cell line. The number of micronuclei did not rank the cell lines in the same order of radiosensitivity as clonogenic cell survival, which showed a surviving fraction at 2 Gy of 0.38 +/- 0.02 for JR8, 0.34 +/- 0.05 for M14 and 0.22 +/- 0.007 for A2780. As regards primary tumour cultures, no correlation was observed between micronucleus induction and surviving fraction at 2 Gy. In conclusion, the discrepancy we observed between micronucleus formation and cell death raises doubts about the potential of the micronucleus assay as a preclinical means to predict radiosensitivity. IMAGES: Nature Publishing Group 1994-12 /pmc/articles/PMC2033705/ /pubmed/7981062 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Villa, R.
Zaffaroni, N.
Gornati, D.
Costa, A.
Silvestrini, R.
Lack of a correlation between micronucleus formation and radiosensitivity in established and primary cultures of human tumours.
title Lack of a correlation between micronucleus formation and radiosensitivity in established and primary cultures of human tumours.
title_full Lack of a correlation between micronucleus formation and radiosensitivity in established and primary cultures of human tumours.
title_fullStr Lack of a correlation between micronucleus formation and radiosensitivity in established and primary cultures of human tumours.
title_full_unstemmed Lack of a correlation between micronucleus formation and radiosensitivity in established and primary cultures of human tumours.
title_short Lack of a correlation between micronucleus formation and radiosensitivity in established and primary cultures of human tumours.
title_sort lack of a correlation between micronucleus formation and radiosensitivity in established and primary cultures of human tumours.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033705/
https://www.ncbi.nlm.nih.gov/pubmed/7981062
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AT gornatid lackofacorrelationbetweenmicronucleusformationandradiosensitivityinestablishedandprimaryculturesofhumantumours
AT costaa lackofacorrelationbetweenmicronucleusformationandradiosensitivityinestablishedandprimaryculturesofhumantumours
AT silvestrinir lackofacorrelationbetweenmicronucleusformationandradiosensitivityinestablishedandprimaryculturesofhumantumours

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