Inhibition of gastric cancer cell proliferation by antisense oligonucleotides targeting the messenger RNA encoding proliferating cell nuclear antigen.

Proliferating cell nuclear antigen (PCNA) is a nuclear protein that regulates DNA synthesis by DNA polymerase delta, and is essential for DNA replication. PCNA expression level is related to the malignancy of gastric cancer cells. Seven different gastric cancer cell lines and two kinds of control ce...

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Autores principales: Sakakura, C., Hagiwara, A., Tsujimoto, H., Ozaki, K., Sakakibara, T., Oyama, T., Ogaki, M., Takahashi, T.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1994
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033708/
https://www.ncbi.nlm.nih.gov/pubmed/7981055
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author Sakakura, C.
Hagiwara, A.
Tsujimoto, H.
Ozaki, K.
Sakakibara, T.
Oyama, T.
Ogaki, M.
Takahashi, T.
author_facet Sakakura, C.
Hagiwara, A.
Tsujimoto, H.
Ozaki, K.
Sakakibara, T.
Oyama, T.
Ogaki, M.
Takahashi, T.
author_sort Sakakura, C.
collection PubMed
description Proliferating cell nuclear antigen (PCNA) is a nuclear protein that regulates DNA synthesis by DNA polymerase delta, and is essential for DNA replication. PCNA expression level is related to the malignancy of gastric cancer cells. Seven different gastric cancer cell lines and two kinds of control cell lines were treated with antisense oligonucleotides complementary to the messenger RNA of PCNA. Treatment of each gastric cancer cell line with antisense oligonucleotides at concentration of 10-40 microM inhibited the cell growth, colony formation and PCNA protein production in a dose-dependent manner, but only affected normal cells slightly. A random sequence oligomer showed no effect. These results show that PCNA is essential for gastric cancer cell proliferation and that the use of synthetic oligonucleotides is an effective way of producing antisense-mediated changes in the behaviour of human gastric cancers. IMAGES:
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spelling pubmed-20337082009-09-10 Inhibition of gastric cancer cell proliferation by antisense oligonucleotides targeting the messenger RNA encoding proliferating cell nuclear antigen. Sakakura, C. Hagiwara, A. Tsujimoto, H. Ozaki, K. Sakakibara, T. Oyama, T. Ogaki, M. Takahashi, T. Br J Cancer Research Article Proliferating cell nuclear antigen (PCNA) is a nuclear protein that regulates DNA synthesis by DNA polymerase delta, and is essential for DNA replication. PCNA expression level is related to the malignancy of gastric cancer cells. Seven different gastric cancer cell lines and two kinds of control cell lines were treated with antisense oligonucleotides complementary to the messenger RNA of PCNA. Treatment of each gastric cancer cell line with antisense oligonucleotides at concentration of 10-40 microM inhibited the cell growth, colony formation and PCNA protein production in a dose-dependent manner, but only affected normal cells slightly. A random sequence oligomer showed no effect. These results show that PCNA is essential for gastric cancer cell proliferation and that the use of synthetic oligonucleotides is an effective way of producing antisense-mediated changes in the behaviour of human gastric cancers. IMAGES: Nature Publishing Group 1994-12 /pmc/articles/PMC2033708/ /pubmed/7981055 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Sakakura, C.
Hagiwara, A.
Tsujimoto, H.
Ozaki, K.
Sakakibara, T.
Oyama, T.
Ogaki, M.
Takahashi, T.
Inhibition of gastric cancer cell proliferation by antisense oligonucleotides targeting the messenger RNA encoding proliferating cell nuclear antigen.
title Inhibition of gastric cancer cell proliferation by antisense oligonucleotides targeting the messenger RNA encoding proliferating cell nuclear antigen.
title_full Inhibition of gastric cancer cell proliferation by antisense oligonucleotides targeting the messenger RNA encoding proliferating cell nuclear antigen.
title_fullStr Inhibition of gastric cancer cell proliferation by antisense oligonucleotides targeting the messenger RNA encoding proliferating cell nuclear antigen.
title_full_unstemmed Inhibition of gastric cancer cell proliferation by antisense oligonucleotides targeting the messenger RNA encoding proliferating cell nuclear antigen.
title_short Inhibition of gastric cancer cell proliferation by antisense oligonucleotides targeting the messenger RNA encoding proliferating cell nuclear antigen.
title_sort inhibition of gastric cancer cell proliferation by antisense oligonucleotides targeting the messenger rna encoding proliferating cell nuclear antigen.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033708/
https://www.ncbi.nlm.nih.gov/pubmed/7981055
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