Bacillus Calmette-Guerin (BCG) enhances monocyte- and lymphocyte-mediated bladder tumour cell killing.

A cytotoxicity assay was used to study the action of bacillus Calmette-Guerin (BCG) and cytokines on four human bladder cancer cell lines. Monocytes and lymphocytes from peripheral blood were incubated with or without BCG or cytokines for 24 h, after which [3H]thymidine-labelled target cells were ad...

Descripción completa

Detalles Bibliográficos
Autores principales: Pryor, K., Goddard, J., Goldstein, D., Stricker, P., Russell, P., Golovsky, D., Penny, R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1995
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033737/
https://www.ncbi.nlm.nih.gov/pubmed/7710947
_version_ 1782136903016906752
author Pryor, K.
Goddard, J.
Goldstein, D.
Stricker, P.
Russell, P.
Golovsky, D.
Penny, R.
author_facet Pryor, K.
Goddard, J.
Goldstein, D.
Stricker, P.
Russell, P.
Golovsky, D.
Penny, R.
author_sort Pryor, K.
collection PubMed
description A cytotoxicity assay was used to study the action of bacillus Calmette-Guerin (BCG) and cytokines on four human bladder cancer cell lines. Monocytes and lymphocytes from peripheral blood were incubated with or without BCG or cytokines for 24 h, after which [3H]thymidine-labelled target cells were added and the 72 h percentage specific release determined. BCG had a direct cytotoxic effect against tumour cells and significantly enhanced monocyte/macrophage and enhanced lymphocyte cytotoxicity against one cell line (UCRU-BL-17). Supernatants (SNs) from BCG-activated monocytes/macrophages and lymphocytes increased the percentage specific release of [3H]thymidine from UCRU-BL-17 cells. Interferon alpha (IFN-alpha) and interleukin 2 (IL-2) were cytotoxic towards UCRU-BL-17. No synergy occurred between BCG and cytokines at the concentrations tested. The results suggest that BCG is superior to IFN-alpha, interferon gamma (IFN-gamma) and IL-2 in enhancing cell-mediated cytotoxicity.
format Text
id pubmed-2033737
institution National Center for Biotechnology Information
language English
publishDate 1995
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-20337372009-09-10 Bacillus Calmette-Guerin (BCG) enhances monocyte- and lymphocyte-mediated bladder tumour cell killing. Pryor, K. Goddard, J. Goldstein, D. Stricker, P. Russell, P. Golovsky, D. Penny, R. Br J Cancer Research Article A cytotoxicity assay was used to study the action of bacillus Calmette-Guerin (BCG) and cytokines on four human bladder cancer cell lines. Monocytes and lymphocytes from peripheral blood were incubated with or without BCG or cytokines for 24 h, after which [3H]thymidine-labelled target cells were added and the 72 h percentage specific release determined. BCG had a direct cytotoxic effect against tumour cells and significantly enhanced monocyte/macrophage and enhanced lymphocyte cytotoxicity against one cell line (UCRU-BL-17). Supernatants (SNs) from BCG-activated monocytes/macrophages and lymphocytes increased the percentage specific release of [3H]thymidine from UCRU-BL-17 cells. Interferon alpha (IFN-alpha) and interleukin 2 (IL-2) were cytotoxic towards UCRU-BL-17. No synergy occurred between BCG and cytokines at the concentrations tested. The results suggest that BCG is superior to IFN-alpha, interferon gamma (IFN-gamma) and IL-2 in enhancing cell-mediated cytotoxicity. Nature Publishing Group 1995-04 /pmc/articles/PMC2033737/ /pubmed/7710947 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Pryor, K.
Goddard, J.
Goldstein, D.
Stricker, P.
Russell, P.
Golovsky, D.
Penny, R.
Bacillus Calmette-Guerin (BCG) enhances monocyte- and lymphocyte-mediated bladder tumour cell killing.
title Bacillus Calmette-Guerin (BCG) enhances monocyte- and lymphocyte-mediated bladder tumour cell killing.
title_full Bacillus Calmette-Guerin (BCG) enhances monocyte- and lymphocyte-mediated bladder tumour cell killing.
title_fullStr Bacillus Calmette-Guerin (BCG) enhances monocyte- and lymphocyte-mediated bladder tumour cell killing.
title_full_unstemmed Bacillus Calmette-Guerin (BCG) enhances monocyte- and lymphocyte-mediated bladder tumour cell killing.
title_short Bacillus Calmette-Guerin (BCG) enhances monocyte- and lymphocyte-mediated bladder tumour cell killing.
title_sort bacillus calmette-guerin (bcg) enhances monocyte- and lymphocyte-mediated bladder tumour cell killing.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033737/
https://www.ncbi.nlm.nih.gov/pubmed/7710947
work_keys_str_mv AT pryork bacilluscalmetteguerinbcgenhancesmonocyteandlymphocytemediatedbladdertumourcellkilling
AT goddardj bacilluscalmetteguerinbcgenhancesmonocyteandlymphocytemediatedbladdertumourcellkilling
AT goldsteind bacilluscalmetteguerinbcgenhancesmonocyteandlymphocytemediatedbladdertumourcellkilling
AT strickerp bacilluscalmetteguerinbcgenhancesmonocyteandlymphocytemediatedbladdertumourcellkilling
AT russellp bacilluscalmetteguerinbcgenhancesmonocyteandlymphocytemediatedbladdertumourcellkilling
AT golovskyd bacilluscalmetteguerinbcgenhancesmonocyteandlymphocytemediatedbladdertumourcellkilling
AT pennyr bacilluscalmetteguerinbcgenhancesmonocyteandlymphocytemediatedbladdertumourcellkilling

Ejemplares similares