Elevated levels of members of the STAT family of transcription factors in breast carcinoma nuclear extracts.

The transcription factor, milk protein binding factor (MPBF/Stat5), is a member of the STAT family of signalling molecules which mediates prolactin signal transduction in lactating mammary gland by binding to GAS (gamma-interferon activation site) DNA elements. We have determined the levels of STAT...

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Detalles Bibliográficos
Autores principales: Watson, C. J., Miller, W. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1995
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033751/
https://www.ncbi.nlm.nih.gov/pubmed/7710952
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author Watson, C. J.
Miller, W. R.
author_facet Watson, C. J.
Miller, W. R.
author_sort Watson, C. J.
collection PubMed
description The transcription factor, milk protein binding factor (MPBF/Stat5), is a member of the STAT family of signalling molecules which mediates prolactin signal transduction in lactating mammary gland by binding to GAS (gamma-interferon activation site) DNA elements. We have determined the levels of STAT factors in nuclear extracts from a variety of human breast tissues including carcinoma and normal 'resting' breast by electrophoretic mobility-shift assay. The results show that the level of STAT binding activity is low in normal 'resting' breast and benign lesions while carcinoma samples have significantly higher (P < 0.01) amounts of STAT binding activity. Supershift analysis suggests that Stat1 and possibly other members of the STAT family of signalling factors, including Stat3, are activated in breast cancer tissues. IMAGES:
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spelling pubmed-20337512009-09-10 Elevated levels of members of the STAT family of transcription factors in breast carcinoma nuclear extracts. Watson, C. J. Miller, W. R. Br J Cancer Research Article The transcription factor, milk protein binding factor (MPBF/Stat5), is a member of the STAT family of signalling molecules which mediates prolactin signal transduction in lactating mammary gland by binding to GAS (gamma-interferon activation site) DNA elements. We have determined the levels of STAT factors in nuclear extracts from a variety of human breast tissues including carcinoma and normal 'resting' breast by electrophoretic mobility-shift assay. The results show that the level of STAT binding activity is low in normal 'resting' breast and benign lesions while carcinoma samples have significantly higher (P < 0.01) amounts of STAT binding activity. Supershift analysis suggests that Stat1 and possibly other members of the STAT family of signalling factors, including Stat3, are activated in breast cancer tissues. IMAGES: Nature Publishing Group 1995-04 /pmc/articles/PMC2033751/ /pubmed/7710952 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Watson, C. J.
Miller, W. R.
Elevated levels of members of the STAT family of transcription factors in breast carcinoma nuclear extracts.
title Elevated levels of members of the STAT family of transcription factors in breast carcinoma nuclear extracts.
title_full Elevated levels of members of the STAT family of transcription factors in breast carcinoma nuclear extracts.
title_fullStr Elevated levels of members of the STAT family of transcription factors in breast carcinoma nuclear extracts.
title_full_unstemmed Elevated levels of members of the STAT family of transcription factors in breast carcinoma nuclear extracts.
title_short Elevated levels of members of the STAT family of transcription factors in breast carcinoma nuclear extracts.
title_sort elevated levels of members of the stat family of transcription factors in breast carcinoma nuclear extracts.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033751/
https://www.ncbi.nlm.nih.gov/pubmed/7710952
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