Loss of heterozygosity at chromosome 11 in breast cancer: association of prognostic factors with genetic alterations.

We examined DNA from 116 female and four male breast cancer patients for loss of heterozygosity (LOH). DNA was analysed by polymerase chain reaction using ten microsatellite markers on chromosome 11. Three distinct regions of LOH were identified: 11p15.5, 11q13 and 11q22-qter with a LOH frequency of...

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Autores principales: Gudmundsson, J., Barkardottir, R. B., Eiriksdottir, G., Baldursson, T., Arason, A., Egilsson, V., Ingvarsson, S.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1995
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033886/
https://www.ncbi.nlm.nih.gov/pubmed/7669583
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author Gudmundsson, J.
Barkardottir, R. B.
Eiriksdottir, G.
Baldursson, T.
Arason, A.
Egilsson, V.
Ingvarsson, S.
author_facet Gudmundsson, J.
Barkardottir, R. B.
Eiriksdottir, G.
Baldursson, T.
Arason, A.
Egilsson, V.
Ingvarsson, S.
author_sort Gudmundsson, J.
collection PubMed
description We examined DNA from 116 female and four male breast cancer patients for loss of heterozygosity (LOH). DNA was analysed by polymerase chain reaction using ten microsatellite markers on chromosome 11. Three distinct regions of LOH were identified: 11p15.5, 11q13 and 11q22-qter with a LOH frequency of 19, 23 and 37-43% respectively. The marker D11S969 showing the highest frequency of LOH (43%) is located at the 11q24.1-q25 region. No previous molecular genetic studies have shown frequent LOH at the region telomeric to q23 on chromosome 11. Southern analysis revealed that LOH at 11q13 was due to amplification, whereas LOH at 11q22qter was due to deletion. LOH at 11p15.5 was associated with paucity of hormone receptor proteins, high S-phase and positive node status. An association was found between LOH at 11q13 and positive node status. LOH at the 11q22-qter region correlated with a high S-phase fraction. A significant association was found between LOH at 11p15 and chromosome regions 17q21 (the BRCA1 region) and 3p. IMAGES:
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spelling pubmed-20338862009-09-10 Loss of heterozygosity at chromosome 11 in breast cancer: association of prognostic factors with genetic alterations. Gudmundsson, J. Barkardottir, R. B. Eiriksdottir, G. Baldursson, T. Arason, A. Egilsson, V. Ingvarsson, S. Br J Cancer Research Article We examined DNA from 116 female and four male breast cancer patients for loss of heterozygosity (LOH). DNA was analysed by polymerase chain reaction using ten microsatellite markers on chromosome 11. Three distinct regions of LOH were identified: 11p15.5, 11q13 and 11q22-qter with a LOH frequency of 19, 23 and 37-43% respectively. The marker D11S969 showing the highest frequency of LOH (43%) is located at the 11q24.1-q25 region. No previous molecular genetic studies have shown frequent LOH at the region telomeric to q23 on chromosome 11. Southern analysis revealed that LOH at 11q13 was due to amplification, whereas LOH at 11q22qter was due to deletion. LOH at 11p15.5 was associated with paucity of hormone receptor proteins, high S-phase and positive node status. An association was found between LOH at 11q13 and positive node status. LOH at the 11q22-qter region correlated with a high S-phase fraction. A significant association was found between LOH at 11p15 and chromosome regions 17q21 (the BRCA1 region) and 3p. IMAGES: Nature Publishing Group 1995-09 /pmc/articles/PMC2033886/ /pubmed/7669583 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Gudmundsson, J.
Barkardottir, R. B.
Eiriksdottir, G.
Baldursson, T.
Arason, A.
Egilsson, V.
Ingvarsson, S.
Loss of heterozygosity at chromosome 11 in breast cancer: association of prognostic factors with genetic alterations.
title Loss of heterozygosity at chromosome 11 in breast cancer: association of prognostic factors with genetic alterations.
title_full Loss of heterozygosity at chromosome 11 in breast cancer: association of prognostic factors with genetic alterations.
title_fullStr Loss of heterozygosity at chromosome 11 in breast cancer: association of prognostic factors with genetic alterations.
title_full_unstemmed Loss of heterozygosity at chromosome 11 in breast cancer: association of prognostic factors with genetic alterations.
title_short Loss of heterozygosity at chromosome 11 in breast cancer: association of prognostic factors with genetic alterations.
title_sort loss of heterozygosity at chromosome 11 in breast cancer: association of prognostic factors with genetic alterations.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033886/
https://www.ncbi.nlm.nih.gov/pubmed/7669583
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