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Processing of long-stored archival cervical smears for human papillomavirus detection by the polymerase chain reaction.

The efficiency of a freeze-thaw method, a proteinase K/Tween 20 lysis method and a guanidinium isothiocyanate/silica beads method for DNA extraction from fixed and Papanicolaou-stained cells from the cervical cancer cell line Siha was measured by beta-globin polymerase chain reaction (PCR). The GTC/...

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Detalles Bibliográficos
Autores principales:	de Roda Husman, A. M., Snijders, P. J., Stel, H. V., van den Brule, A. J., Meijer, C. J., Walboomers, J. M.
Formato:	Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 1995
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033993/ https://www.ncbi.nlm.nih.gov/pubmed/7543772

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