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Processing of long-stored archival cervical smears for human papillomavirus detection by the polymerase chain reaction.
The efficiency of a freeze-thaw method, a proteinase K/Tween 20 lysis method and a guanidinium isothiocyanate/silica beads method for DNA extraction from fixed and Papanicolaou-stained cells from the cervical cancer cell line Siha was measured by beta-globin polymerase chain reaction (PCR). The GTC/...
Autores principales: | de Roda Husman, A. M., Snijders, P. J., Stel, H. V., van den Brule, A. J., Meijer, C. J., Walboomers, J. M. |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
1995
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2033993/ https://www.ncbi.nlm.nih.gov/pubmed/7543772 |
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