Determination of oestrogen responsiveness of breast cancer by competitive reverse transcription-polymerase chain reaction.

Competitive polymerase chain reaction assays have been developed for the quantitation of oestrogen receptor mRNA and two oestrogen-regulated mRNAs (progesterone receptor and pNR-2/pS2) in breast cancer cells. These assays are more sensitive than traditional hybridisation techniques, do not require t...

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Autores principales: Carr, M., May, F. E., Lennard, T. W., Westley, B. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1995
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034093/
https://www.ncbi.nlm.nih.gov/pubmed/8519655
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author Carr, M.
May, F. E.
Lennard, T. W.
Westley, B. R.
author_facet Carr, M.
May, F. E.
Lennard, T. W.
Westley, B. R.
author_sort Carr, M.
collection PubMed
description Competitive polymerase chain reaction assays have been developed for the quantitation of oestrogen receptor mRNA and two oestrogen-regulated mRNAs (progesterone receptor and pNR-2/pS2) in breast cancer cells. These assays are more sensitive than traditional hybridisation techniques, do not require the use of radioisotopes, measure absolute amounts of messenger RNAs and can be used to measure the expression of mRNAs in small numbers of tumour cells obtained by fine-needle aspiration (FNA). These assays should prove useful for predicting the hormone responsiveness of breast cancer from tumour cells obtained by FNA at diagnosis and could be particularly useful in the management of elderly/frail patients who receive primary tamoxifen, or in other patients for whom tumour tissue for standard biochemical measurements is not available. IMAGES:
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spelling pubmed-20340932009-09-10 Determination of oestrogen responsiveness of breast cancer by competitive reverse transcription-polymerase chain reaction. Carr, M. May, F. E. Lennard, T. W. Westley, B. R. Br J Cancer Research Article Competitive polymerase chain reaction assays have been developed for the quantitation of oestrogen receptor mRNA and two oestrogen-regulated mRNAs (progesterone receptor and pNR-2/pS2) in breast cancer cells. These assays are more sensitive than traditional hybridisation techniques, do not require the use of radioisotopes, measure absolute amounts of messenger RNAs and can be used to measure the expression of mRNAs in small numbers of tumour cells obtained by fine-needle aspiration (FNA). These assays should prove useful for predicting the hormone responsiveness of breast cancer from tumour cells obtained by FNA at diagnosis and could be particularly useful in the management of elderly/frail patients who receive primary tamoxifen, or in other patients for whom tumour tissue for standard biochemical measurements is not available. IMAGES: Nature Publishing Group 1995-12 /pmc/articles/PMC2034093/ /pubmed/8519655 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Carr, M.
May, F. E.
Lennard, T. W.
Westley, B. R.
Determination of oestrogen responsiveness of breast cancer by competitive reverse transcription-polymerase chain reaction.
title Determination of oestrogen responsiveness of breast cancer by competitive reverse transcription-polymerase chain reaction.
title_full Determination of oestrogen responsiveness of breast cancer by competitive reverse transcription-polymerase chain reaction.
title_fullStr Determination of oestrogen responsiveness of breast cancer by competitive reverse transcription-polymerase chain reaction.
title_full_unstemmed Determination of oestrogen responsiveness of breast cancer by competitive reverse transcription-polymerase chain reaction.
title_short Determination of oestrogen responsiveness of breast cancer by competitive reverse transcription-polymerase chain reaction.
title_sort determination of oestrogen responsiveness of breast cancer by competitive reverse transcription-polymerase chain reaction.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034093/
https://www.ncbi.nlm.nih.gov/pubmed/8519655
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