TP53 gene mutations and protein accumulation in primary vaginal carcinomas.

Primary carcinomas from 46 patients were screened for TP53 alterations. Immunohistochemistry demonstrated nuclear TP53 protein accumulation in 22 (48%) cases using the polyclonal CM1 antiserum, whereas 15 (33%) cases showed positive nuclear staining with the mononuclear antibody PAb 1801. Constant d...

Descripción completa

Detalles Bibliográficos
Autores principales: Skomedal, H., Kristensen, G., Helland, A., Nesland, J. M., Kooi, S., Børresen, A. L., Holm, R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group 1995
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034131/
https://www.ncbi.nlm.nih.gov/pubmed/7599041
_version_ 1782136989755113472
author Skomedal, H.
Kristensen, G.
Helland, A.
Nesland, J. M.
Kooi, S.
Børresen, A. L.
Holm, R.
author_facet Skomedal, H.
Kristensen, G.
Helland, A.
Nesland, J. M.
Kooi, S.
Børresen, A. L.
Holm, R.
author_sort Skomedal, H.
collection PubMed
description Primary carcinomas from 46 patients were screened for TP53 alterations. Immunohistochemistry demonstrated nuclear TP53 protein accumulation in 22 (48%) cases using the polyclonal CM1 antiserum, whereas 15 (33%) cases showed positive nuclear staining with the mononuclear antibody PAb 1801. Constant denaturant gel electrophoresis (CDGE) was used to screen 27 of the vaginal carcinomas for mutations in the conserved regions of the TP53 gene (exons 5-8). Six of these tumours (22%) contained mutations: four were found in exon 5 and two in exon 8. A total of 50% of the primary vaginal carcinomas carried a TP53 alteration. These results indicate that TP53 abnormalities may be involved in the development of these tumours. However, there was no significant association between TP53 abnormalities and survival. IMAGES:
format Text
id pubmed-2034131
institution National Center for Biotechnology Information
language English
publishDate 1995
publisher Nature Publishing Group
record_format MEDLINE/PubMed
spelling pubmed-20341312009-09-10 TP53 gene mutations and protein accumulation in primary vaginal carcinomas. Skomedal, H. Kristensen, G. Helland, A. Nesland, J. M. Kooi, S. Børresen, A. L. Holm, R. Br J Cancer Research Article Primary carcinomas from 46 patients were screened for TP53 alterations. Immunohistochemistry demonstrated nuclear TP53 protein accumulation in 22 (48%) cases using the polyclonal CM1 antiserum, whereas 15 (33%) cases showed positive nuclear staining with the mononuclear antibody PAb 1801. Constant denaturant gel electrophoresis (CDGE) was used to screen 27 of the vaginal carcinomas for mutations in the conserved regions of the TP53 gene (exons 5-8). Six of these tumours (22%) contained mutations: four were found in exon 5 and two in exon 8. A total of 50% of the primary vaginal carcinomas carried a TP53 alteration. These results indicate that TP53 abnormalities may be involved in the development of these tumours. However, there was no significant association between TP53 abnormalities and survival. IMAGES: Nature Publishing Group 1995-07 /pmc/articles/PMC2034131/ /pubmed/7599041 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Skomedal, H.
Kristensen, G.
Helland, A.
Nesland, J. M.
Kooi, S.
Børresen, A. L.
Holm, R.
TP53 gene mutations and protein accumulation in primary vaginal carcinomas.
title TP53 gene mutations and protein accumulation in primary vaginal carcinomas.
title_full TP53 gene mutations and protein accumulation in primary vaginal carcinomas.
title_fullStr TP53 gene mutations and protein accumulation in primary vaginal carcinomas.
title_full_unstemmed TP53 gene mutations and protein accumulation in primary vaginal carcinomas.
title_short TP53 gene mutations and protein accumulation in primary vaginal carcinomas.
title_sort tp53 gene mutations and protein accumulation in primary vaginal carcinomas.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034131/
https://www.ncbi.nlm.nih.gov/pubmed/7599041
work_keys_str_mv AT skomedalh tp53genemutationsandproteinaccumulationinprimaryvaginalcarcinomas
AT kristenseng tp53genemutationsandproteinaccumulationinprimaryvaginalcarcinomas
AT hellanda tp53genemutationsandproteinaccumulationinprimaryvaginalcarcinomas
AT neslandjm tp53genemutationsandproteinaccumulationinprimaryvaginalcarcinomas
AT koois tp53genemutationsandproteinaccumulationinprimaryvaginalcarcinomas
AT børresenal tp53genemutationsandproteinaccumulationinprimaryvaginalcarcinomas
AT holmr tp53genemutationsandproteinaccumulationinprimaryvaginalcarcinomas

Ejemplares similares