Cargando…
Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression
Lesions in the template DNA strand block the progression of the replication fork. In the yeast Saccharomyces cerevisiae, replication through DNA lesions is mediated by different Rad6-Rad18-dependent means, which include translesion synthesis and a Rad5-dependent postreplicational repair pathway that...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Cell Press
2007
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034406/ https://www.ncbi.nlm.nih.gov/pubmed/17936713 http://dx.doi.org/10.1016/j.molcel.2007.07.030 |
_version_ | 1782137004444614656 |
---|---|
author | Blastyák, András Pintér, Lajos Unk, Ildiko Prakash, Louise Prakash, Satya Haracska, Lajos |
author_facet | Blastyák, András Pintér, Lajos Unk, Ildiko Prakash, Louise Prakash, Satya Haracska, Lajos |
author_sort | Blastyák, András |
collection | PubMed |
description | Lesions in the template DNA strand block the progression of the replication fork. In the yeast Saccharomyces cerevisiae, replication through DNA lesions is mediated by different Rad6-Rad18-dependent means, which include translesion synthesis and a Rad5-dependent postreplicational repair pathway that repairs the discontinuities that form in the DNA synthesized from damaged templates. Although translesion synthesis is well characterized, little is known about the mechanisms that modulate Rad5-dependent postreplicational repair. Here we show that yeast Rad5 has a DNA helicase activity that is specialized for replication fork regression. On model replication fork structures, Rad5 concertedly unwinds and anneals the nascent and the parental strands without exposing extended single-stranded regions. These observations provide insight into the mechanism of postreplicational repair in which Rad5 action promotes template switching for error-free damage bypass. |
format | Text |
id | pubmed-2034406 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Cell Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20344062007-10-24 Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression Blastyák, András Pintér, Lajos Unk, Ildiko Prakash, Louise Prakash, Satya Haracska, Lajos Mol Cell Short Article Lesions in the template DNA strand block the progression of the replication fork. In the yeast Saccharomyces cerevisiae, replication through DNA lesions is mediated by different Rad6-Rad18-dependent means, which include translesion synthesis and a Rad5-dependent postreplicational repair pathway that repairs the discontinuities that form in the DNA synthesized from damaged templates. Although translesion synthesis is well characterized, little is known about the mechanisms that modulate Rad5-dependent postreplicational repair. Here we show that yeast Rad5 has a DNA helicase activity that is specialized for replication fork regression. On model replication fork structures, Rad5 concertedly unwinds and anneals the nascent and the parental strands without exposing extended single-stranded regions. These observations provide insight into the mechanism of postreplicational repair in which Rad5 action promotes template switching for error-free damage bypass. Cell Press 2007-10-12 /pmc/articles/PMC2034406/ /pubmed/17936713 http://dx.doi.org/10.1016/j.molcel.2007.07.030 Text en . https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license |
spellingShingle | Short Article Blastyák, András Pintér, Lajos Unk, Ildiko Prakash, Louise Prakash, Satya Haracska, Lajos Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression |
title | Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression |
title_full | Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression |
title_fullStr | Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression |
title_full_unstemmed | Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression |
title_short | Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression |
title_sort | yeast rad5 protein required for postreplication repair has a dna helicase activity specific for replication fork regression |
topic | Short Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034406/ https://www.ncbi.nlm.nih.gov/pubmed/17936713 http://dx.doi.org/10.1016/j.molcel.2007.07.030 |
work_keys_str_mv | AT blastyakandras yeastrad5proteinrequiredforpostreplicationrepairhasadnahelicaseactivityspecificforreplicationforkregression AT pinterlajos yeastrad5proteinrequiredforpostreplicationrepairhasadnahelicaseactivityspecificforreplicationforkregression AT unkildiko yeastrad5proteinrequiredforpostreplicationrepairhasadnahelicaseactivityspecificforreplicationforkregression AT prakashlouise yeastrad5proteinrequiredforpostreplicationrepairhasadnahelicaseactivityspecificforreplicationforkregression AT prakashsatya yeastrad5proteinrequiredforpostreplicationrepairhasadnahelicaseactivityspecificforreplicationforkregression AT haracskalajos yeastrad5proteinrequiredforpostreplicationrepairhasadnahelicaseactivityspecificforreplicationforkregression |