Cargando…

Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression

Lesions in the template DNA strand block the progression of the replication fork. In the yeast Saccharomyces cerevisiae, replication through DNA lesions is mediated by different Rad6-Rad18-dependent means, which include translesion synthesis and a Rad5-dependent postreplicational repair pathway that...

Descripción completa

Detalles Bibliográficos
Autores principales: Blastyák, András, Pintér, Lajos, Unk, Ildiko, Prakash, Louise, Prakash, Satya, Haracska, Lajos
Formato: Texto
Lenguaje:English
Publicado: Cell Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034406/
https://www.ncbi.nlm.nih.gov/pubmed/17936713
http://dx.doi.org/10.1016/j.molcel.2007.07.030
_version_ 1782137004444614656
author Blastyák, András
Pintér, Lajos
Unk, Ildiko
Prakash, Louise
Prakash, Satya
Haracska, Lajos
author_facet Blastyák, András
Pintér, Lajos
Unk, Ildiko
Prakash, Louise
Prakash, Satya
Haracska, Lajos
author_sort Blastyák, András
collection PubMed
description Lesions in the template DNA strand block the progression of the replication fork. In the yeast Saccharomyces cerevisiae, replication through DNA lesions is mediated by different Rad6-Rad18-dependent means, which include translesion synthesis and a Rad5-dependent postreplicational repair pathway that repairs the discontinuities that form in the DNA synthesized from damaged templates. Although translesion synthesis is well characterized, little is known about the mechanisms that modulate Rad5-dependent postreplicational repair. Here we show that yeast Rad5 has a DNA helicase activity that is specialized for replication fork regression. On model replication fork structures, Rad5 concertedly unwinds and anneals the nascent and the parental strands without exposing extended single-stranded regions. These observations provide insight into the mechanism of postreplicational repair in which Rad5 action promotes template switching for error-free damage bypass.
format Text
id pubmed-2034406
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher Cell Press
record_format MEDLINE/PubMed
spelling pubmed-20344062007-10-24 Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression Blastyák, András Pintér, Lajos Unk, Ildiko Prakash, Louise Prakash, Satya Haracska, Lajos Mol Cell Short Article Lesions in the template DNA strand block the progression of the replication fork. In the yeast Saccharomyces cerevisiae, replication through DNA lesions is mediated by different Rad6-Rad18-dependent means, which include translesion synthesis and a Rad5-dependent postreplicational repair pathway that repairs the discontinuities that form in the DNA synthesized from damaged templates. Although translesion synthesis is well characterized, little is known about the mechanisms that modulate Rad5-dependent postreplicational repair. Here we show that yeast Rad5 has a DNA helicase activity that is specialized for replication fork regression. On model replication fork structures, Rad5 concertedly unwinds and anneals the nascent and the parental strands without exposing extended single-stranded regions. These observations provide insight into the mechanism of postreplicational repair in which Rad5 action promotes template switching for error-free damage bypass. Cell Press 2007-10-12 /pmc/articles/PMC2034406/ /pubmed/17936713 http://dx.doi.org/10.1016/j.molcel.2007.07.030 Text en . https://creativecommons.org/licenses/by/3.0/ Open Access under CC BY 3.0 (https://creativecommons.org/licenses/by/3.0/) license
spellingShingle Short Article
Blastyák, András
Pintér, Lajos
Unk, Ildiko
Prakash, Louise
Prakash, Satya
Haracska, Lajos
Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression
title Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression
title_full Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression
title_fullStr Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression
title_full_unstemmed Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression
title_short Yeast Rad5 Protein Required for Postreplication Repair Has a DNA Helicase Activity Specific for Replication Fork Regression
title_sort yeast rad5 protein required for postreplication repair has a dna helicase activity specific for replication fork regression
topic Short Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034406/
https://www.ncbi.nlm.nih.gov/pubmed/17936713
http://dx.doi.org/10.1016/j.molcel.2007.07.030
work_keys_str_mv AT blastyakandras yeastrad5proteinrequiredforpostreplicationrepairhasadnahelicaseactivityspecificforreplicationforkregression
AT pinterlajos yeastrad5proteinrequiredforpostreplicationrepairhasadnahelicaseactivityspecificforreplicationforkregression
AT unkildiko yeastrad5proteinrequiredforpostreplicationrepairhasadnahelicaseactivityspecificforreplicationforkregression
AT prakashlouise yeastrad5proteinrequiredforpostreplicationrepairhasadnahelicaseactivityspecificforreplicationforkregression
AT prakashsatya yeastrad5proteinrequiredforpostreplicationrepairhasadnahelicaseactivityspecificforreplicationforkregression
AT haracskalajos yeastrad5proteinrequiredforpostreplicationrepairhasadnahelicaseactivityspecificforreplicationforkregression