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CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges

Although the integrase (IntDOT) of the Bacteroides conjugative transposon CTnDOT has been classified as a member of the tyrosine recombinase family, the reaction it catalyzes appears to differ in some features from reactions catalyzed by other tyrosine recombinases. We tested the ability of IntDOT t...

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Detalles Bibliográficos
Autores principales: Malanowska, Karolina, Yoneji, Sumiko, Salyers, Abigail A., Gardner, Jeffrey F.
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034462/
https://www.ncbi.nlm.nih.gov/pubmed/17720706
http://dx.doi.org/10.1093/nar/gkm637
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author Malanowska, Karolina
Yoneji, Sumiko
Salyers, Abigail A.
Gardner, Jeffrey F.
author_facet Malanowska, Karolina
Yoneji, Sumiko
Salyers, Abigail A.
Gardner, Jeffrey F.
author_sort Malanowska, Karolina
collection PubMed
description Although the integrase (IntDOT) of the Bacteroides conjugative transposon CTnDOT has been classified as a member of the tyrosine recombinase family, the reaction it catalyzes appears to differ in some features from reactions catalyzed by other tyrosine recombinases. We tested the ability of IntDOT to cleave and ligate activated attDOT substrates in the presence of mismatches. Unlike other tyrosine recombinases, the results revealed that IntDOT is able to perform ligation reactions even when all the bases within the crossover region are mispaired. We also show that there is a strong bias in the order of strand exchanges during integrative recombination. The top strands are exchanged first in reactions that appear to require 2 bp of homology between the partner sites adjacent to the sites of cleavage. The bottom strands are exchanged next in reactions that do not require homology between the partner sites. This mode of coordination of strand exchanges is unique among tyrosine recombinases.
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spelling pubmed-20344622007-10-24 CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges Malanowska, Karolina Yoneji, Sumiko Salyers, Abigail A. Gardner, Jeffrey F. Nucleic Acids Res Nucleic Acid Enzymes Although the integrase (IntDOT) of the Bacteroides conjugative transposon CTnDOT has been classified as a member of the tyrosine recombinase family, the reaction it catalyzes appears to differ in some features from reactions catalyzed by other tyrosine recombinases. We tested the ability of IntDOT to cleave and ligate activated attDOT substrates in the presence of mismatches. Unlike other tyrosine recombinases, the results revealed that IntDOT is able to perform ligation reactions even when all the bases within the crossover region are mispaired. We also show that there is a strong bias in the order of strand exchanges during integrative recombination. The top strands are exchanged first in reactions that appear to require 2 bp of homology between the partner sites adjacent to the sites of cleavage. The bottom strands are exchanged next in reactions that do not require homology between the partner sites. This mode of coordination of strand exchanges is unique among tyrosine recombinases. Oxford University Press 2007-09 2007-08-24 /pmc/articles/PMC2034462/ /pubmed/17720706 http://dx.doi.org/10.1093/nar/gkm637 Text en © 2007 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Nucleic Acid Enzymes
Malanowska, Karolina
Yoneji, Sumiko
Salyers, Abigail A.
Gardner, Jeffrey F.
CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges
title CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges
title_full CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges
title_fullStr CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges
title_full_unstemmed CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges
title_short CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges
title_sort ctndot integrase performs ordered homology-dependent and homology-independent strand exchanges
topic Nucleic Acid Enzymes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034462/
https://www.ncbi.nlm.nih.gov/pubmed/17720706
http://dx.doi.org/10.1093/nar/gkm637
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