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Altered target site specificity variants of the I-PpoI His-Cys box homing endonuclease

We used a yeast one-hybrid assay to isolate and characterize variants of the eukaryotic homing endonuclease I-PpoI that were able to bind a mutant, cleavage-resistant I-PpoI target or ‘homing’ site DNA in vivo. Native I-PpoI recognizes and cleaves a semi-palindromic 15-bp target site with high speci...

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Detalles Bibliográficos
Autores principales:	Eklund, Jennifer L., Ulge, Umut Y., Eastberg, Jennifer, Monnat, Raymond J.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2007
Materias:	Nucleic Acid Enzymes
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034468/ https://www.ncbi.nlm.nih.gov/pubmed/17720708 http://dx.doi.org/10.1093/nar/gkm624

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