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The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA

DNA damage checkpoints are signal transduction pathways that are activated after genotoxic insults to protect genomic integrity. At the site of DNA damage, ‘mediator’ proteins are in charge of recruiting ‘signal transducers’ to molecules ‘sensing’ the damage. Budding yeast Rad9, fission yeast Crb2 a...

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Autores principales: Lancelot, Nathalie, Charier, Gaëlle, Couprie, Joël, Duband-Goulet, Isabelle, Alpha-Bazin, Béatrice, Quémeneur, Eric, Ma, Emilie, Marsolier-Kergoat, Marie-Claude, Ropars, Virginie, Charbonnier, Jean-Baptiste, Miron, Simona, Craescu, Constantin T., Callebaut, Isabelle, Gilquin, Bernard, Zinn-Justin, Sophie
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034471/
https://www.ncbi.nlm.nih.gov/pubmed/17726056
http://dx.doi.org/10.1093/nar/gkm607
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author Lancelot, Nathalie
Charier, Gaëlle
Couprie, Joël
Duband-Goulet, Isabelle
Alpha-Bazin, Béatrice
Quémeneur, Eric
Ma, Emilie
Marsolier-Kergoat, Marie-Claude
Ropars, Virginie
Charbonnier, Jean-Baptiste
Miron, Simona
Craescu, Constantin T.
Callebaut, Isabelle
Gilquin, Bernard
Zinn-Justin, Sophie
author_facet Lancelot, Nathalie
Charier, Gaëlle
Couprie, Joël
Duband-Goulet, Isabelle
Alpha-Bazin, Béatrice
Quémeneur, Eric
Ma, Emilie
Marsolier-Kergoat, Marie-Claude
Ropars, Virginie
Charbonnier, Jean-Baptiste
Miron, Simona
Craescu, Constantin T.
Callebaut, Isabelle
Gilquin, Bernard
Zinn-Justin, Sophie
author_sort Lancelot, Nathalie
collection PubMed
description DNA damage checkpoints are signal transduction pathways that are activated after genotoxic insults to protect genomic integrity. At the site of DNA damage, ‘mediator’ proteins are in charge of recruiting ‘signal transducers’ to molecules ‘sensing’ the damage. Budding yeast Rad9, fission yeast Crb2 and metazoan 53BP1 are presented as mediators involved in the activation of checkpoint kinases. Here we show that, despite low sequence conservation, Rad9 exhibits a tandem tudor domain structurally close to those found in human/mouse 53BP1 and fission yeast Crb2. Moreover, this region is important for the resistance of Saccharomyces cerevisiae to different genotoxic stresses. It does not mediate direct binding to a histone H3 peptide dimethylated on K79, nor to a histone H4 peptide dimethylated on lysine 20, as was demonstrated for 53BP1. However, the tandem tudor region of Rad9 directly interacts with single-stranded DNA and double-stranded DNAs of various lengths and sequences through a positively charged region absent from 53BP1 and Crb2 but present in several yeast Rad9 homologs. Our results argue that the tandem tudor domains of Rad9, Crb2 and 53BP1 mediate chromatin binding next to double-strand breaks. However, their modes of chromatin recognition are different, suggesting that the corresponding interactions are differently regulated.
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spelling pubmed-20344712007-10-24 The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA Lancelot, Nathalie Charier, Gaëlle Couprie, Joël Duband-Goulet, Isabelle Alpha-Bazin, Béatrice Quémeneur, Eric Ma, Emilie Marsolier-Kergoat, Marie-Claude Ropars, Virginie Charbonnier, Jean-Baptiste Miron, Simona Craescu, Constantin T. Callebaut, Isabelle Gilquin, Bernard Zinn-Justin, Sophie Nucleic Acids Res Structural Biology DNA damage checkpoints are signal transduction pathways that are activated after genotoxic insults to protect genomic integrity. At the site of DNA damage, ‘mediator’ proteins are in charge of recruiting ‘signal transducers’ to molecules ‘sensing’ the damage. Budding yeast Rad9, fission yeast Crb2 and metazoan 53BP1 are presented as mediators involved in the activation of checkpoint kinases. Here we show that, despite low sequence conservation, Rad9 exhibits a tandem tudor domain structurally close to those found in human/mouse 53BP1 and fission yeast Crb2. Moreover, this region is important for the resistance of Saccharomyces cerevisiae to different genotoxic stresses. It does not mediate direct binding to a histone H3 peptide dimethylated on K79, nor to a histone H4 peptide dimethylated on lysine 20, as was demonstrated for 53BP1. However, the tandem tudor region of Rad9 directly interacts with single-stranded DNA and double-stranded DNAs of various lengths and sequences through a positively charged region absent from 53BP1 and Crb2 but present in several yeast Rad9 homologs. Our results argue that the tandem tudor domains of Rad9, Crb2 and 53BP1 mediate chromatin binding next to double-strand breaks. However, their modes of chromatin recognition are different, suggesting that the corresponding interactions are differently regulated. Oxford University Press 2007-09 2007-08-28 /pmc/articles/PMC2034471/ /pubmed/17726056 http://dx.doi.org/10.1093/nar/gkm607 Text en © 2007 The Author(s) http://creativecommons.org/licenses/by-nc/2.0/uk/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Structural Biology
Lancelot, Nathalie
Charier, Gaëlle
Couprie, Joël
Duband-Goulet, Isabelle
Alpha-Bazin, Béatrice
Quémeneur, Eric
Ma, Emilie
Marsolier-Kergoat, Marie-Claude
Ropars, Virginie
Charbonnier, Jean-Baptiste
Miron, Simona
Craescu, Constantin T.
Callebaut, Isabelle
Gilquin, Bernard
Zinn-Justin, Sophie
The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA
title The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA
title_full The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA
title_fullStr The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA
title_full_unstemmed The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA
title_short The checkpoint Saccharomyces cerevisiae Rad9 protein contains a tandem tudor domain that recognizes DNA
title_sort checkpoint saccharomyces cerevisiae rad9 protein contains a tandem tudor domain that recognizes dna
topic Structural Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034471/
https://www.ncbi.nlm.nih.gov/pubmed/17726056
http://dx.doi.org/10.1093/nar/gkm607
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