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Enhancement of clonogenicity of human multiple myeloma by dendritic cells

Infiltration by dendritic cells (DCs) is a common feature of most human tumors. Prior studies evaluating the interaction of DCs with tumors have focused largely on their immunologic properties (for review see Banchereau, J., and R.M. Steinman. 1998. Nature. 392:245–252). In this study, we show that...

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Autores principales: Kukreja, Anjli, Hutchinson, Aisha, Dhodapkar, Kavita, Mazumder, Amitabha, Vesole, David, Angitapalli, Revathi, Jagannath, Sundar, Dhodapkar, Madhav V.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034506/
https://www.ncbi.nlm.nih.gov/pubmed/16880256
http://dx.doi.org/10.1084/jem.20052136
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author Kukreja, Anjli
Hutchinson, Aisha
Dhodapkar, Kavita
Mazumder, Amitabha
Vesole, David
Angitapalli, Revathi
Jagannath, Sundar
Dhodapkar, Madhav V.
author_facet Kukreja, Anjli
Hutchinson, Aisha
Dhodapkar, Kavita
Mazumder, Amitabha
Vesole, David
Angitapalli, Revathi
Jagannath, Sundar
Dhodapkar, Madhav V.
author_sort Kukreja, Anjli
collection PubMed
description Infiltration by dendritic cells (DCs) is a common feature of most human tumors. Prior studies evaluating the interaction of DCs with tumors have focused largely on their immunologic properties (for review see Banchereau, J., and R.M. Steinman. 1998. Nature. 392:245–252). In this study, we show that the clonogenicity of several human tumor cell lines and primary tumor cells from myeloma patients is enhanced by their interactions with DCs. Myeloma cells cultured in the presence of DCs have an altered phenotype with an increased proportion of cells lacking terminal plasma cell differentiation marker CD138. DC–tumor interaction also leads to the up-regulation of B cell lymphoma 6 expression in myeloma cells. Effects of DCs on myeloma cells are inhibited by blockade of the receptor activator of NF-kB (RANK)–RANK ligand and B cell–activating factor–APRIL (a proliferation-inducing ligand)-mediated interactions. Together, these data suggest that tumor–DC interactions may directly impact the biology of human tumors, particularly multiple myeloma, and may be a target for therapeutic intervention.
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spelling pubmed-20345062007-12-13 Enhancement of clonogenicity of human multiple myeloma by dendritic cells Kukreja, Anjli Hutchinson, Aisha Dhodapkar, Kavita Mazumder, Amitabha Vesole, David Angitapalli, Revathi Jagannath, Sundar Dhodapkar, Madhav V. J Exp Med Brief Definitive Reports Infiltration by dendritic cells (DCs) is a common feature of most human tumors. Prior studies evaluating the interaction of DCs with tumors have focused largely on their immunologic properties (for review see Banchereau, J., and R.M. Steinman. 1998. Nature. 392:245–252). In this study, we show that the clonogenicity of several human tumor cell lines and primary tumor cells from myeloma patients is enhanced by their interactions with DCs. Myeloma cells cultured in the presence of DCs have an altered phenotype with an increased proportion of cells lacking terminal plasma cell differentiation marker CD138. DC–tumor interaction also leads to the up-regulation of B cell lymphoma 6 expression in myeloma cells. Effects of DCs on myeloma cells are inhibited by blockade of the receptor activator of NF-kB (RANK)–RANK ligand and B cell–activating factor–APRIL (a proliferation-inducing ligand)-mediated interactions. Together, these data suggest that tumor–DC interactions may directly impact the biology of human tumors, particularly multiple myeloma, and may be a target for therapeutic intervention. The Rockefeller University Press 2006-08-07 /pmc/articles/PMC2034506/ /pubmed/16880256 http://dx.doi.org/10.1084/jem.20052136 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Brief Definitive Reports
Kukreja, Anjli
Hutchinson, Aisha
Dhodapkar, Kavita
Mazumder, Amitabha
Vesole, David
Angitapalli, Revathi
Jagannath, Sundar
Dhodapkar, Madhav V.
Enhancement of clonogenicity of human multiple myeloma by dendritic cells
title Enhancement of clonogenicity of human multiple myeloma by dendritic cells
title_full Enhancement of clonogenicity of human multiple myeloma by dendritic cells
title_fullStr Enhancement of clonogenicity of human multiple myeloma by dendritic cells
title_full_unstemmed Enhancement of clonogenicity of human multiple myeloma by dendritic cells
title_short Enhancement of clonogenicity of human multiple myeloma by dendritic cells
title_sort enhancement of clonogenicity of human multiple myeloma by dendritic cells
topic Brief Definitive Reports
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2034506/
https://www.ncbi.nlm.nih.gov/pubmed/16880256
http://dx.doi.org/10.1084/jem.20052136
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