Cargando…

The activation of nicotinic acetylcholine receptors enhances the inhibitory synaptic transmission in the deep dorsal horn neurons of the adult rat spinal cord

Somatosensory information can be modulated by nicotinic acetylcholine receptors (nAChRs) in the superficial dorsal horn of the spinal cord. Nonetheless, the functional significance of nAChRs in the deep dorsal horn of adult animals remains unclear. Using whole-cell patch-clamp recordings from lamina...

Descripción completa

Detalles Bibliográficos
Autores principales: Takeda, Daisuke, Nakatsuka, Terumasa, Gu, Jianguo G, Yoshida, Munehito
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2039725/
https://www.ncbi.nlm.nih.gov/pubmed/17894865
http://dx.doi.org/10.1186/1744-8069-3-26
_version_ 1782137034596417536
author Takeda, Daisuke
Nakatsuka, Terumasa
Gu, Jianguo G
Yoshida, Munehito
author_facet Takeda, Daisuke
Nakatsuka, Terumasa
Gu, Jianguo G
Yoshida, Munehito
author_sort Takeda, Daisuke
collection PubMed
description Somatosensory information can be modulated by nicotinic acetylcholine receptors (nAChRs) in the superficial dorsal horn of the spinal cord. Nonetheless, the functional significance of nAChRs in the deep dorsal horn of adult animals remains unclear. Using whole-cell patch-clamp recordings from lamina V neurons in the adult rat spinal cord, we investigated whether the activation of nAChRs could modulate the inhibitory synaptic transmission in the deep dorsal horn. In the presence of CNQX and APV to block excitatory glutamatergic synaptic transmission, bath applications of nicotine (100 μM) significantly increased the frequency of spontaneous inhibitory postsynaptic currents (sIPSCs) in almost all neurons tested. The effect of nicotine was mimicked by N-methyl-4-(3-pyridinyl)-3-butene-1-amine (RJR-2403, 100 μM), an α4β2-nAChR agonist, and was also mimicked by choline (10 mM), an α7-nAChR agonist. The effect of nicotine was completely blocked by the nAChR antagonist mecamylamine (5 μM). In the presence of tetrodotoxin (0.5 μM), nicotine (100 μM) significantly increased the miniature IPSC frequency. On the other hand, RJR-2403 (100 μM) or choline (10 mM) did not affect miniature IPSCs. The application of nicotine (100 μM) also evoked a large inward current in all lamina V neurons tested when cells were held at -60 mV. Similarly, RJR-2403 (100 μM) induced inward currents in the majority of lamina V neurons examined. On the other hand, choline (10 mM) did not elicit any detectable whole-cell currents. These results suggest that several nAChR subtypes are expressed on the presynaptic terminals, preterminals, and neuronal cell bodies within lamina V and that these nAChRs are involved in the modulation of inhibitory synaptic activity in the deep dorsal horn of the spinal cord.
format Text
id pubmed-2039725
institution National Center for Biotechnology Information
language English
publishDate 2007
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-20397252007-10-20 The activation of nicotinic acetylcholine receptors enhances the inhibitory synaptic transmission in the deep dorsal horn neurons of the adult rat spinal cord Takeda, Daisuke Nakatsuka, Terumasa Gu, Jianguo G Yoshida, Munehito Mol Pain Research Somatosensory information can be modulated by nicotinic acetylcholine receptors (nAChRs) in the superficial dorsal horn of the spinal cord. Nonetheless, the functional significance of nAChRs in the deep dorsal horn of adult animals remains unclear. Using whole-cell patch-clamp recordings from lamina V neurons in the adult rat spinal cord, we investigated whether the activation of nAChRs could modulate the inhibitory synaptic transmission in the deep dorsal horn. In the presence of CNQX and APV to block excitatory glutamatergic synaptic transmission, bath applications of nicotine (100 μM) significantly increased the frequency of spontaneous inhibitory postsynaptic currents (sIPSCs) in almost all neurons tested. The effect of nicotine was mimicked by N-methyl-4-(3-pyridinyl)-3-butene-1-amine (RJR-2403, 100 μM), an α4β2-nAChR agonist, and was also mimicked by choline (10 mM), an α7-nAChR agonist. The effect of nicotine was completely blocked by the nAChR antagonist mecamylamine (5 μM). In the presence of tetrodotoxin (0.5 μM), nicotine (100 μM) significantly increased the miniature IPSC frequency. On the other hand, RJR-2403 (100 μM) or choline (10 mM) did not affect miniature IPSCs. The application of nicotine (100 μM) also evoked a large inward current in all lamina V neurons tested when cells were held at -60 mV. Similarly, RJR-2403 (100 μM) induced inward currents in the majority of lamina V neurons examined. On the other hand, choline (10 mM) did not elicit any detectable whole-cell currents. These results suggest that several nAChR subtypes are expressed on the presynaptic terminals, preterminals, and neuronal cell bodies within lamina V and that these nAChRs are involved in the modulation of inhibitory synaptic activity in the deep dorsal horn of the spinal cord. BioMed Central 2007-09-25 /pmc/articles/PMC2039725/ /pubmed/17894865 http://dx.doi.org/10.1186/1744-8069-3-26 Text en Copyright © 2007 Takeda et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Takeda, Daisuke
Nakatsuka, Terumasa
Gu, Jianguo G
Yoshida, Munehito
The activation of nicotinic acetylcholine receptors enhances the inhibitory synaptic transmission in the deep dorsal horn neurons of the adult rat spinal cord
title The activation of nicotinic acetylcholine receptors enhances the inhibitory synaptic transmission in the deep dorsal horn neurons of the adult rat spinal cord
title_full The activation of nicotinic acetylcholine receptors enhances the inhibitory synaptic transmission in the deep dorsal horn neurons of the adult rat spinal cord
title_fullStr The activation of nicotinic acetylcholine receptors enhances the inhibitory synaptic transmission in the deep dorsal horn neurons of the adult rat spinal cord
title_full_unstemmed The activation of nicotinic acetylcholine receptors enhances the inhibitory synaptic transmission in the deep dorsal horn neurons of the adult rat spinal cord
title_short The activation of nicotinic acetylcholine receptors enhances the inhibitory synaptic transmission in the deep dorsal horn neurons of the adult rat spinal cord
title_sort activation of nicotinic acetylcholine receptors enhances the inhibitory synaptic transmission in the deep dorsal horn neurons of the adult rat spinal cord
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2039725/
https://www.ncbi.nlm.nih.gov/pubmed/17894865
http://dx.doi.org/10.1186/1744-8069-3-26
work_keys_str_mv AT takedadaisuke theactivationofnicotinicacetylcholinereceptorsenhancestheinhibitorysynaptictransmissioninthedeepdorsalhornneuronsoftheadultratspinalcord
AT nakatsukaterumasa theactivationofnicotinicacetylcholinereceptorsenhancestheinhibitorysynaptictransmissioninthedeepdorsalhornneuronsoftheadultratspinalcord
AT gujianguog theactivationofnicotinicacetylcholinereceptorsenhancestheinhibitorysynaptictransmissioninthedeepdorsalhornneuronsoftheadultratspinalcord
AT yoshidamunehito theactivationofnicotinicacetylcholinereceptorsenhancestheinhibitorysynaptictransmissioninthedeepdorsalhornneuronsoftheadultratspinalcord
AT takedadaisuke activationofnicotinicacetylcholinereceptorsenhancestheinhibitorysynaptictransmissioninthedeepdorsalhornneuronsoftheadultratspinalcord
AT nakatsukaterumasa activationofnicotinicacetylcholinereceptorsenhancestheinhibitorysynaptictransmissioninthedeepdorsalhornneuronsoftheadultratspinalcord
AT gujianguog activationofnicotinicacetylcholinereceptorsenhancestheinhibitorysynaptictransmissioninthedeepdorsalhornneuronsoftheadultratspinalcord
AT yoshidamunehito activationofnicotinicacetylcholinereceptorsenhancestheinhibitorysynaptictransmissioninthedeepdorsalhornneuronsoftheadultratspinalcord