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Construction and evaluation of multisite recombinatorial (Gateway) cloning vectors for Gram-positive bacteria
BACKGROUND: The Gateway recombinatorial cloning system allows easy and rapid joining of DNA fragments. Here we report the construction and evaluation of three different Gram-positive vectors that can be used with the Multisite Gateway cloning system to rapidly produce new gene arrangements in plasmi...
Autores principales: | Perehinec, Tania M, Qazi, Saara NA, Gaddipati, Sanyasi R, Salisbury, Vyvyan, Rees, Catherine ED, Hill, Philip J |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2039747/ https://www.ncbi.nlm.nih.gov/pubmed/17880697 http://dx.doi.org/10.1186/1471-2199-8-80 |
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