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Mechanical Stress Induces Biotic and Abiotic Stress Responses via a Novel cis-Element
Plants are continuously exposed to a myriad of abiotic and biotic stresses. However, the molecular mechanisms by which these stress signals are perceived and transduced are poorly understood. To begin to identify primary stress signal transduction components, we have focused on genes that respond ra...
Autores principales: | , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2039767/ https://www.ncbi.nlm.nih.gov/pubmed/17953483 http://dx.doi.org/10.1371/journal.pgen.0030172 |
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author | Walley, Justin W Coughlan, Sean Hudson, Matthew E Covington, Michael F Kaspi, Roy Banu, Gopalan Harmer, Stacey L Dehesh, Katayoon |
author_facet | Walley, Justin W Coughlan, Sean Hudson, Matthew E Covington, Michael F Kaspi, Roy Banu, Gopalan Harmer, Stacey L Dehesh, Katayoon |
author_sort | Walley, Justin W |
collection | PubMed |
description | Plants are continuously exposed to a myriad of abiotic and biotic stresses. However, the molecular mechanisms by which these stress signals are perceived and transduced are poorly understood. To begin to identify primary stress signal transduction components, we have focused on genes that respond rapidly (within 5 min) to stress signals. Because it has been hypothesized that detection of physical stress is a mechanism common to mounting a response against a broad range of environmental stresses, we have utilized mechanical wounding as the stress stimulus and performed whole genome microarray analysis of Arabidopsis thaliana leaf tissue. This led to the identification of a number of rapid wound responsive (RWR) genes. Comparison of RWR genes with published abiotic and biotic stress microarray datasets demonstrates a large overlap across a wide range of environmental stresses. Interestingly, RWR genes also exhibit a striking level and pattern of circadian regulation, with induced and repressed genes displaying antiphasic rhythms. Using bioinformatic analysis, we identified a novel motif overrepresented in the promoters of RWR genes, herein designated as the Rapid Stress Response Element (RSRE). We demonstrate in transgenic plants that multimerized RSREs are sufficient to confer a rapid response to both biotic and abiotic stresses in vivo, thereby establishing the functional involvement of this motif in primary transcriptional stress responses. Collectively, our data provide evidence for a novel cis-element that is distributed across the promoters of an array of diverse stress-responsive genes, poised to respond immediately and coordinately to stress signals. This structure suggests that plants may have a transcriptional network resembling the general stress signaling pathway in yeast and that the RSRE element may provide the key to this coordinate regulation. |
format | Text |
id | pubmed-2039767 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-20397672007-10-25 Mechanical Stress Induces Biotic and Abiotic Stress Responses via a Novel cis-Element Walley, Justin W Coughlan, Sean Hudson, Matthew E Covington, Michael F Kaspi, Roy Banu, Gopalan Harmer, Stacey L Dehesh, Katayoon PLoS Genet Research Article Plants are continuously exposed to a myriad of abiotic and biotic stresses. However, the molecular mechanisms by which these stress signals are perceived and transduced are poorly understood. To begin to identify primary stress signal transduction components, we have focused on genes that respond rapidly (within 5 min) to stress signals. Because it has been hypothesized that detection of physical stress is a mechanism common to mounting a response against a broad range of environmental stresses, we have utilized mechanical wounding as the stress stimulus and performed whole genome microarray analysis of Arabidopsis thaliana leaf tissue. This led to the identification of a number of rapid wound responsive (RWR) genes. Comparison of RWR genes with published abiotic and biotic stress microarray datasets demonstrates a large overlap across a wide range of environmental stresses. Interestingly, RWR genes also exhibit a striking level and pattern of circadian regulation, with induced and repressed genes displaying antiphasic rhythms. Using bioinformatic analysis, we identified a novel motif overrepresented in the promoters of RWR genes, herein designated as the Rapid Stress Response Element (RSRE). We demonstrate in transgenic plants that multimerized RSREs are sufficient to confer a rapid response to both biotic and abiotic stresses in vivo, thereby establishing the functional involvement of this motif in primary transcriptional stress responses. Collectively, our data provide evidence for a novel cis-element that is distributed across the promoters of an array of diverse stress-responsive genes, poised to respond immediately and coordinately to stress signals. This structure suggests that plants may have a transcriptional network resembling the general stress signaling pathway in yeast and that the RSRE element may provide the key to this coordinate regulation. Public Library of Science 2007-10 2007-10-19 /pmc/articles/PMC2039767/ /pubmed/17953483 http://dx.doi.org/10.1371/journal.pgen.0030172 Text en © 2007 Walley et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Walley, Justin W Coughlan, Sean Hudson, Matthew E Covington, Michael F Kaspi, Roy Banu, Gopalan Harmer, Stacey L Dehesh, Katayoon Mechanical Stress Induces Biotic and Abiotic Stress Responses via a Novel cis-Element |
title | Mechanical Stress Induces Biotic and Abiotic Stress Responses via a Novel cis-Element |
title_full | Mechanical Stress Induces Biotic and Abiotic Stress Responses via a Novel cis-Element |
title_fullStr | Mechanical Stress Induces Biotic and Abiotic Stress Responses via a Novel cis-Element |
title_full_unstemmed | Mechanical Stress Induces Biotic and Abiotic Stress Responses via a Novel cis-Element |
title_short | Mechanical Stress Induces Biotic and Abiotic Stress Responses via a Novel cis-Element |
title_sort | mechanical stress induces biotic and abiotic stress responses via a novel cis-element |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2039767/ https://www.ncbi.nlm.nih.gov/pubmed/17953483 http://dx.doi.org/10.1371/journal.pgen.0030172 |
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