Rapid Assembly of Multiple-Exon cDNA Directly from Genomic DNA

BACKGROUND: Polymerase chain reaction (PCR) is extensively applied in gene cloning. But due to the existence of introns, low copy number of particular genes and high complexity of the eukaryotic genome, it is usually impossible to amplify and clone a gene as a full-length sequence directly from the...

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Detalles Bibliográficos
Autores principales: An, Xiaoping, Lu, Jun, Huang, Jian-dong, Zhang, Baozhong, Liu, Dabin, Zhang, Xin, Chen, Jinhui, Zhou, Yusen, Tong, Yigang
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2007
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2048664/
https://www.ncbi.nlm.nih.gov/pubmed/18000550
http://dx.doi.org/10.1371/journal.pone.0001179

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