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mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer

BACKGROUND: Colorectal cancer develops through two main genetic instability pathways characterized by distinct pathologic features and clinical outcome. RESULTS: We investigated colon cancer samples (23 characterized by microsatellite stability, MSS, and 16 by high microsatellite instability, MSI-H)...

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Autores principales: Lanza, Giovanni, Ferracin, Manuela, Gafà, Roberta, Veronese, Angelo, Spizzo, Riccardo, Pichiorri, Flavia, Liu, Chang-gong, Calin, George A, Croce, Carlo M, Negrini, Massimo
Formato: Texto
Lenguaje:English
Publicado: BioMed Central|1 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2048978/
https://www.ncbi.nlm.nih.gov/pubmed/17716371
http://dx.doi.org/10.1186/1476-4598-6-54
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author Lanza, Giovanni
Ferracin, Manuela
Gafà, Roberta
Veronese, Angelo
Spizzo, Riccardo
Pichiorri, Flavia
Liu, Chang-gong
Calin, George A
Croce, Carlo M
Negrini, Massimo
author_facet Lanza, Giovanni
Ferracin, Manuela
Gafà, Roberta
Veronese, Angelo
Spizzo, Riccardo
Pichiorri, Flavia
Liu, Chang-gong
Calin, George A
Croce, Carlo M
Negrini, Massimo
author_sort Lanza, Giovanni
collection PubMed
description BACKGROUND: Colorectal cancer develops through two main genetic instability pathways characterized by distinct pathologic features and clinical outcome. RESULTS: We investigated colon cancer samples (23 characterized by microsatellite stability, MSS, and 16 by high microsatellite instability, MSI-H) for genome-wide expression of microRNA (miRNA) and mRNA. Based on combined miRNA and mRNA gene expression, a molecular signature consisting of twenty seven differentially expressed genes, inclusive of 8 miRNAs, could correctly distinguish MSI-H versus MSS colon cancer samples. Among the differentially expressed miRNAs, various members of the oncogenic miR-17-92 family were significantly up-regulated in MSS cancers. The majority of protein coding genes were also up-regulated in MSS cancers. Their functional classification revealed that they were most frequently associated with cell cycle, DNA replication, recombination, repair, gastrointestinal disease and immune response. CONCLUSION: This is the first report that indicates the existence of differences in miRNA expression between MSS versus MSI-H colorectal cancers. In addition, the work suggests that the combination of mRNA/miRNA expression signatures may represent a general approach for improving bio-molecular classification of human cancer.
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spelling pubmed-20489782007-11-03 mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer Lanza, Giovanni Ferracin, Manuela Gafà, Roberta Veronese, Angelo Spizzo, Riccardo Pichiorri, Flavia Liu, Chang-gong Calin, George A Croce, Carlo M Negrini, Massimo Mol Cancer Research BACKGROUND: Colorectal cancer develops through two main genetic instability pathways characterized by distinct pathologic features and clinical outcome. RESULTS: We investigated colon cancer samples (23 characterized by microsatellite stability, MSS, and 16 by high microsatellite instability, MSI-H) for genome-wide expression of microRNA (miRNA) and mRNA. Based on combined miRNA and mRNA gene expression, a molecular signature consisting of twenty seven differentially expressed genes, inclusive of 8 miRNAs, could correctly distinguish MSI-H versus MSS colon cancer samples. Among the differentially expressed miRNAs, various members of the oncogenic miR-17-92 family were significantly up-regulated in MSS cancers. The majority of protein coding genes were also up-regulated in MSS cancers. Their functional classification revealed that they were most frequently associated with cell cycle, DNA replication, recombination, repair, gastrointestinal disease and immune response. CONCLUSION: This is the first report that indicates the existence of differences in miRNA expression between MSS versus MSI-H colorectal cancers. In addition, the work suggests that the combination of mRNA/miRNA expression signatures may represent a general approach for improving bio-molecular classification of human cancer. BioMed Central|1 2007-08-23 /pmc/articles/PMC2048978/ /pubmed/17716371 http://dx.doi.org/10.1186/1476-4598-6-54 Text en Copyright © 2007 Lanza et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Lanza, Giovanni
Ferracin, Manuela
Gafà, Roberta
Veronese, Angelo
Spizzo, Riccardo
Pichiorri, Flavia
Liu, Chang-gong
Calin, George A
Croce, Carlo M
Negrini, Massimo
mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer
title mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer
title_full mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer
title_fullStr mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer
title_full_unstemmed mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer
title_short mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer
title_sort mrna/microrna gene expression profile in microsatellite unstable colorectal cancer
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2048978/
https://www.ncbi.nlm.nih.gov/pubmed/17716371
http://dx.doi.org/10.1186/1476-4598-6-54
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