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mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer
BACKGROUND: Colorectal cancer develops through two main genetic instability pathways characterized by distinct pathologic features and clinical outcome. RESULTS: We investigated colon cancer samples (23 characterized by microsatellite stability, MSS, and 16 by high microsatellite instability, MSI-H)...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
BioMed Central|1
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2048978/ https://www.ncbi.nlm.nih.gov/pubmed/17716371 http://dx.doi.org/10.1186/1476-4598-6-54 |
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author | Lanza, Giovanni Ferracin, Manuela Gafà, Roberta Veronese, Angelo Spizzo, Riccardo Pichiorri, Flavia Liu, Chang-gong Calin, George A Croce, Carlo M Negrini, Massimo |
author_facet | Lanza, Giovanni Ferracin, Manuela Gafà, Roberta Veronese, Angelo Spizzo, Riccardo Pichiorri, Flavia Liu, Chang-gong Calin, George A Croce, Carlo M Negrini, Massimo |
author_sort | Lanza, Giovanni |
collection | PubMed |
description | BACKGROUND: Colorectal cancer develops through two main genetic instability pathways characterized by distinct pathologic features and clinical outcome. RESULTS: We investigated colon cancer samples (23 characterized by microsatellite stability, MSS, and 16 by high microsatellite instability, MSI-H) for genome-wide expression of microRNA (miRNA) and mRNA. Based on combined miRNA and mRNA gene expression, a molecular signature consisting of twenty seven differentially expressed genes, inclusive of 8 miRNAs, could correctly distinguish MSI-H versus MSS colon cancer samples. Among the differentially expressed miRNAs, various members of the oncogenic miR-17-92 family were significantly up-regulated in MSS cancers. The majority of protein coding genes were also up-regulated in MSS cancers. Their functional classification revealed that they were most frequently associated with cell cycle, DNA replication, recombination, repair, gastrointestinal disease and immune response. CONCLUSION: This is the first report that indicates the existence of differences in miRNA expression between MSS versus MSI-H colorectal cancers. In addition, the work suggests that the combination of mRNA/miRNA expression signatures may represent a general approach for improving bio-molecular classification of human cancer. |
format | Text |
id | pubmed-2048978 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | BioMed Central|1 |
record_format | MEDLINE/PubMed |
spelling | pubmed-20489782007-11-03 mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer Lanza, Giovanni Ferracin, Manuela Gafà, Roberta Veronese, Angelo Spizzo, Riccardo Pichiorri, Flavia Liu, Chang-gong Calin, George A Croce, Carlo M Negrini, Massimo Mol Cancer Research BACKGROUND: Colorectal cancer develops through two main genetic instability pathways characterized by distinct pathologic features and clinical outcome. RESULTS: We investigated colon cancer samples (23 characterized by microsatellite stability, MSS, and 16 by high microsatellite instability, MSI-H) for genome-wide expression of microRNA (miRNA) and mRNA. Based on combined miRNA and mRNA gene expression, a molecular signature consisting of twenty seven differentially expressed genes, inclusive of 8 miRNAs, could correctly distinguish MSI-H versus MSS colon cancer samples. Among the differentially expressed miRNAs, various members of the oncogenic miR-17-92 family were significantly up-regulated in MSS cancers. The majority of protein coding genes were also up-regulated in MSS cancers. Their functional classification revealed that they were most frequently associated with cell cycle, DNA replication, recombination, repair, gastrointestinal disease and immune response. CONCLUSION: This is the first report that indicates the existence of differences in miRNA expression between MSS versus MSI-H colorectal cancers. In addition, the work suggests that the combination of mRNA/miRNA expression signatures may represent a general approach for improving bio-molecular classification of human cancer. BioMed Central|1 2007-08-23 /pmc/articles/PMC2048978/ /pubmed/17716371 http://dx.doi.org/10.1186/1476-4598-6-54 Text en Copyright © 2007 Lanza et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License ( (http://creativecommons.org/licenses/by/2.0) ), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Lanza, Giovanni Ferracin, Manuela Gafà, Roberta Veronese, Angelo Spizzo, Riccardo Pichiorri, Flavia Liu, Chang-gong Calin, George A Croce, Carlo M Negrini, Massimo mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer |
title | mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer |
title_full | mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer |
title_fullStr | mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer |
title_full_unstemmed | mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer |
title_short | mRNA/microRNA gene expression profile in microsatellite unstable colorectal cancer |
title_sort | mrna/microrna gene expression profile in microsatellite unstable colorectal cancer |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2048978/ https://www.ncbi.nlm.nih.gov/pubmed/17716371 http://dx.doi.org/10.1186/1476-4598-6-54 |
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