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Wild-type p53-mediated down-modulation of interleukin 15 and interleukin 15 receptors in human rhabdomyosarcoma cells.

We recently reported that rhabdomyosarcoma cell lines express and secrete interleukin 15 (IL-15), a tightly regulated cytokine with IL-2-like activity. To test whether the p53-impaired function that is frequently found in this tumour type could play a role in the IL-15 production, wild-type p53 gene...

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Autores principales: De Giovanni, C., Nanni, P., Sacchi, A., Soddu, S., Manni, I., D'Orazi, G., Bulfone-Paus, S., Pohl, T., Landuzzi, L., Nicoletti, G., Frabetti, F., Rossi, I., Lollini, P. L.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group|1 1998
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063235/
https://www.ncbi.nlm.nih.gov/pubmed/9862562
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author De Giovanni, C.
Nanni, P.
Sacchi, A.
Soddu, S.
Manni, I.
D'Orazi, G.
Bulfone-Paus, S.
Pohl, T.
Landuzzi, L.
Nicoletti, G.
Frabetti, F.
Rossi, I.
Lollini, P. L.
author_facet De Giovanni, C.
Nanni, P.
Sacchi, A.
Soddu, S.
Manni, I.
D'Orazi, G.
Bulfone-Paus, S.
Pohl, T.
Landuzzi, L.
Nicoletti, G.
Frabetti, F.
Rossi, I.
Lollini, P. L.
author_sort De Giovanni, C.
collection PubMed
description We recently reported that rhabdomyosarcoma cell lines express and secrete interleukin 15 (IL-15), a tightly regulated cytokine with IL-2-like activity. To test whether the p53-impaired function that is frequently found in this tumour type could play a role in the IL-15 production, wild-type p53 gene was transduced in the human rhabdomyosarcoma cell line RD (which harbours a mutated p53 gene), and its effect on proliferation and expression of IL-15 was studied. Arrest of proliferation was induced by wild-type p53; increased proportions of G1-arrested cells and of apoptotic cells were observed. A marked down-modulation of IL-15 expression, at both the mRNA and protein level, was found in p53-transduced cells. Because a direct effect of IL-15 on normal muscle cells has been reported, the presence of IL-15 membrane receptors was studied by cytofluorometric analysis. Rhabdomyosarcoma cells showed IL-15 membrane receptors, which are down-modulated by wild-type p53 transfected gene. In conclusion, wild-type p53 transduction in human rhabdomyosarcoma cells induces the down-modulation of both IL-15 production and IL-15 receptor expression. IMAGES:
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spelling pubmed-20632352009-09-10 Wild-type p53-mediated down-modulation of interleukin 15 and interleukin 15 receptors in human rhabdomyosarcoma cells. De Giovanni, C. Nanni, P. Sacchi, A. Soddu, S. Manni, I. D'Orazi, G. Bulfone-Paus, S. Pohl, T. Landuzzi, L. Nicoletti, G. Frabetti, F. Rossi, I. Lollini, P. L. Br J Cancer Research Article We recently reported that rhabdomyosarcoma cell lines express and secrete interleukin 15 (IL-15), a tightly regulated cytokine with IL-2-like activity. To test whether the p53-impaired function that is frequently found in this tumour type could play a role in the IL-15 production, wild-type p53 gene was transduced in the human rhabdomyosarcoma cell line RD (which harbours a mutated p53 gene), and its effect on proliferation and expression of IL-15 was studied. Arrest of proliferation was induced by wild-type p53; increased proportions of G1-arrested cells and of apoptotic cells were observed. A marked down-modulation of IL-15 expression, at both the mRNA and protein level, was found in p53-transduced cells. Because a direct effect of IL-15 on normal muscle cells has been reported, the presence of IL-15 membrane receptors was studied by cytofluorometric analysis. Rhabdomyosarcoma cells showed IL-15 membrane receptors, which are down-modulated by wild-type p53 transfected gene. In conclusion, wild-type p53 transduction in human rhabdomyosarcoma cells induces the down-modulation of both IL-15 production and IL-15 receptor expression. IMAGES: Nature Publishing Group|1 1998-12 /pmc/articles/PMC2063235/ /pubmed/9862562 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
De Giovanni, C.
Nanni, P.
Sacchi, A.
Soddu, S.
Manni, I.
D'Orazi, G.
Bulfone-Paus, S.
Pohl, T.
Landuzzi, L.
Nicoletti, G.
Frabetti, F.
Rossi, I.
Lollini, P. L.
Wild-type p53-mediated down-modulation of interleukin 15 and interleukin 15 receptors in human rhabdomyosarcoma cells.
title Wild-type p53-mediated down-modulation of interleukin 15 and interleukin 15 receptors in human rhabdomyosarcoma cells.
title_full Wild-type p53-mediated down-modulation of interleukin 15 and interleukin 15 receptors in human rhabdomyosarcoma cells.
title_fullStr Wild-type p53-mediated down-modulation of interleukin 15 and interleukin 15 receptors in human rhabdomyosarcoma cells.
title_full_unstemmed Wild-type p53-mediated down-modulation of interleukin 15 and interleukin 15 receptors in human rhabdomyosarcoma cells.
title_short Wild-type p53-mediated down-modulation of interleukin 15 and interleukin 15 receptors in human rhabdomyosarcoma cells.
title_sort wild-type p53-mediated down-modulation of interleukin 15 and interleukin 15 receptors in human rhabdomyosarcoma cells.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063235/
https://www.ncbi.nlm.nih.gov/pubmed/9862562
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