Five newly established oesophageal carcinoma cell lines: phenotypic and immunological characterization.

The derivation of permanent cell lines from 40 resected oesophageal carcinomas has been attempted. Five long-term lines have been established from three adenocarcinomas, one mixed carcinoma and one squamous carcinoma. Molecular and cellular analyses have been carried out on the lines and clones deri...

Descripción completa

Detalles Bibliográficos
Autores principales: Rockett, J. C., Larkin, K., Darnton, S. J., Morris, A. G., Matthews, H. R.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group|1 1997
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063267/
https://www.ncbi.nlm.nih.gov/pubmed/9010035
_version_ 1782137301006024704
author Rockett, J. C.
Larkin, K.
Darnton, S. J.
Morris, A. G.
Matthews, H. R.
author_facet Rockett, J. C.
Larkin, K.
Darnton, S. J.
Morris, A. G.
Matthews, H. R.
author_sort Rockett, J. C.
collection PubMed
description The derivation of permanent cell lines from 40 resected oesophageal carcinomas has been attempted. Five long-term lines have been established from three adenocarcinomas, one mixed carcinoma and one squamous carcinoma. Molecular and cellular analyses have been carried out on the lines and clones derived from them. Karyotype analysis indicates genetic variation among the clones. HLA-A, -B and -C is expressed constitutively, but not HLA-DR. ICAM-1-expressing phenotypes may have arisen during adaptation to long-term culture. All lines are capable of response to interferon-gamma (IFN-gamma) and all produce transforming growth factor beta 1 (TGF-beta 1). Two lines are resistant to the inhibitory growth effects of the latter, possibly contributing to malignancy. It is anticipated that these lines, originating from histologically different carcinomas, will provide a valuable, continuous resource for the investigation and treatment of these aggressive tumours. IMAGES:
format Text
id pubmed-2063267
institution National Center for Biotechnology Information
language English
publishDate 1997
publisher Nature Publishing Group|1
record_format MEDLINE/PubMed
spelling pubmed-20632672009-09-10 Five newly established oesophageal carcinoma cell lines: phenotypic and immunological characterization. Rockett, J. C. Larkin, K. Darnton, S. J. Morris, A. G. Matthews, H. R. Br J Cancer Research Article The derivation of permanent cell lines from 40 resected oesophageal carcinomas has been attempted. Five long-term lines have been established from three adenocarcinomas, one mixed carcinoma and one squamous carcinoma. Molecular and cellular analyses have been carried out on the lines and clones derived from them. Karyotype analysis indicates genetic variation among the clones. HLA-A, -B and -C is expressed constitutively, but not HLA-DR. ICAM-1-expressing phenotypes may have arisen during adaptation to long-term culture. All lines are capable of response to interferon-gamma (IFN-gamma) and all produce transforming growth factor beta 1 (TGF-beta 1). Two lines are resistant to the inhibitory growth effects of the latter, possibly contributing to malignancy. It is anticipated that these lines, originating from histologically different carcinomas, will provide a valuable, continuous resource for the investigation and treatment of these aggressive tumours. IMAGES: Nature Publishing Group|1 1997 /pmc/articles/PMC2063267/ /pubmed/9010035 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Rockett, J. C.
Larkin, K.
Darnton, S. J.
Morris, A. G.
Matthews, H. R.
Five newly established oesophageal carcinoma cell lines: phenotypic and immunological characterization.
title Five newly established oesophageal carcinoma cell lines: phenotypic and immunological characterization.
title_full Five newly established oesophageal carcinoma cell lines: phenotypic and immunological characterization.
title_fullStr Five newly established oesophageal carcinoma cell lines: phenotypic and immunological characterization.
title_full_unstemmed Five newly established oesophageal carcinoma cell lines: phenotypic and immunological characterization.
title_short Five newly established oesophageal carcinoma cell lines: phenotypic and immunological characterization.
title_sort five newly established oesophageal carcinoma cell lines: phenotypic and immunological characterization.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063267/
https://www.ncbi.nlm.nih.gov/pubmed/9010035
work_keys_str_mv AT rockettjc fivenewlyestablishedoesophagealcarcinomacelllinesphenotypicandimmunologicalcharacterization
AT larkink fivenewlyestablishedoesophagealcarcinomacelllinesphenotypicandimmunologicalcharacterization
AT darntonsj fivenewlyestablishedoesophagealcarcinomacelllinesphenotypicandimmunologicalcharacterization
AT morrisag fivenewlyestablishedoesophagealcarcinomacelllinesphenotypicandimmunologicalcharacterization
AT matthewshr fivenewlyestablishedoesophagealcarcinomacelllinesphenotypicandimmunologicalcharacterization

Ejemplares similares