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Alterations in DNA methylation are early, but not initial, events in ovarian tumorigenesis.

We compared global levels of DNA methylation as well as methylation of a specific locus (MyoD1) in ovarian cystadenomas, ovarian tumours of low malignant potential (LMP) and ovarian carcinomas to investigate the association between changes in DNA methylation and ovarian tumour development. As we rea...

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Autores principales: Cheng, P., Schmutte, C., Cofer, K. F., Felix, J. C., Yu, M. C., Dubeau, L.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group|1 1997
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063379/
https://www.ncbi.nlm.nih.gov/pubmed/9020485
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author Cheng, P.
Schmutte, C.
Cofer, K. F.
Felix, J. C.
Yu, M. C.
Dubeau, L.
author_facet Cheng, P.
Schmutte, C.
Cofer, K. F.
Felix, J. C.
Yu, M. C.
Dubeau, L.
author_sort Cheng, P.
collection PubMed
description We compared global levels of DNA methylation as well as methylation of a specific locus (MyoD1) in ovarian cystadenomas, ovarian tumours of low malignant potential (LMP) and ovarian carcinomas to investigate the association between changes in DNA methylation and ovarian tumour development. As we realized that cystadenomas showed different methylation patterns from both LMP tumours and carcinomas, we verified their monoclonal origin as a means of confirming their true neoplastic nature. High-pressure liquid chromatographic (HPLC) analyses showed that global methylation levels in LMP tumours and carcinomas were 21% and 25% lower than in cystadenomas respectively (P = 0.0001 by one-way variance analysis). Changes in the methylation status of the MyoD1 locus were not seen in any of ten cystadenomas analysed but were present in five of ten LMP tumours and in five of ten carcinomas (P = 0.03). These findings suggest that alterations in DNA methylation are absent (or at least not as extensive) in ovarian cystadenomas, but are present in LMP tumours, the phenotypic features of which are intermediate between those of benign and malignant ovarian tumours. The results also emphasize the merit of distinguishing ovarian LMP tumours from cystadenomas, in spite of their similar clinical characteristics. IMAGES:
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spelling pubmed-20633792009-09-10 Alterations in DNA methylation are early, but not initial, events in ovarian tumorigenesis. Cheng, P. Schmutte, C. Cofer, K. F. Felix, J. C. Yu, M. C. Dubeau, L. Br J Cancer Research Article We compared global levels of DNA methylation as well as methylation of a specific locus (MyoD1) in ovarian cystadenomas, ovarian tumours of low malignant potential (LMP) and ovarian carcinomas to investigate the association between changes in DNA methylation and ovarian tumour development. As we realized that cystadenomas showed different methylation patterns from both LMP tumours and carcinomas, we verified their monoclonal origin as a means of confirming their true neoplastic nature. High-pressure liquid chromatographic (HPLC) analyses showed that global methylation levels in LMP tumours and carcinomas were 21% and 25% lower than in cystadenomas respectively (P = 0.0001 by one-way variance analysis). Changes in the methylation status of the MyoD1 locus were not seen in any of ten cystadenomas analysed but were present in five of ten LMP tumours and in five of ten carcinomas (P = 0.03). These findings suggest that alterations in DNA methylation are absent (or at least not as extensive) in ovarian cystadenomas, but are present in LMP tumours, the phenotypic features of which are intermediate between those of benign and malignant ovarian tumours. The results also emphasize the merit of distinguishing ovarian LMP tumours from cystadenomas, in spite of their similar clinical characteristics. IMAGES: Nature Publishing Group|1 1997 /pmc/articles/PMC2063379/ /pubmed/9020485 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Cheng, P.
Schmutte, C.
Cofer, K. F.
Felix, J. C.
Yu, M. C.
Dubeau, L.
Alterations in DNA methylation are early, but not initial, events in ovarian tumorigenesis.
title Alterations in DNA methylation are early, but not initial, events in ovarian tumorigenesis.
title_full Alterations in DNA methylation are early, but not initial, events in ovarian tumorigenesis.
title_fullStr Alterations in DNA methylation are early, but not initial, events in ovarian tumorigenesis.
title_full_unstemmed Alterations in DNA methylation are early, but not initial, events in ovarian tumorigenesis.
title_short Alterations in DNA methylation are early, but not initial, events in ovarian tumorigenesis.
title_sort alterations in dna methylation are early, but not initial, events in ovarian tumorigenesis.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063379/
https://www.ncbi.nlm.nih.gov/pubmed/9020485
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