Detection of alphafetoprotein-expressing cells in the blood of patients with hepatoma and hepatitis.

The presence of tumour cells in the blood circulation may predict disease recurrence and metastasis. We have evaluated the specificity and sensitivity of detecting hepatoma cells in blood using nested polymerase chain reaction with primers specific for the alphafetoprotein (AFP) gene. The nested pol...

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Autores principales: Jiang, S. Y., Shyu, R. Y., Huang, M. F., Tang, H. S., Young, T. H., Roffler, S. R., Chiou, Y. S., Yeh, M. Y.
Formato: Texto
Lenguaje:English
Publicado: Nature Publishing Group|1 1997
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063396/
https://www.ncbi.nlm.nih.gov/pubmed/9062418
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author Jiang, S. Y.
Shyu, R. Y.
Huang, M. F.
Tang, H. S.
Young, T. H.
Roffler, S. R.
Chiou, Y. S.
Yeh, M. Y.
author_facet Jiang, S. Y.
Shyu, R. Y.
Huang, M. F.
Tang, H. S.
Young, T. H.
Roffler, S. R.
Chiou, Y. S.
Yeh, M. Y.
author_sort Jiang, S. Y.
collection PubMed
description The presence of tumour cells in the blood circulation may predict disease recurrence and metastasis. We have evaluated the specificity and sensitivity of detecting hepatoma cells in blood using nested polymerase chain reaction with primers specific for the alphafetoprotein (AFP) gene. The nested polymerase chain reaction amplified a 270-base pair AFP DNA fragment from cDNA of Hep 3B hepatoma cells. In a reconstitution experiment, AFP mRNA was detected from peripheral mononuclear cells isolated from 10 ml of blood containing as few as ten Hep 3B cells. Peripheral mononuclear cells from the blood of 20 hepatoma patients were analysed, and 19 patients showed positive AFP mRNA expression. Seven of 13 samples from hepatitis patients also showed positive AFP mRNA expression. All five paired samples of peripheral blood or umbilical cord blood from pregnant mothers and their babies, respectively, showed positive AFP expression. None of 22 control samples was positive. The presence of AFP mRNA in the blood of hepatitis or hepatoma patients suggests the presence of circulating hepatoma cells or hepatocytes in the circulation. The high incidence of AFP mRNA in the blood of hepatoma patients supports the notion of early haematogenous spreading of the disease. IMAGES:
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spelling pubmed-20633962009-09-10 Detection of alphafetoprotein-expressing cells in the blood of patients with hepatoma and hepatitis. Jiang, S. Y. Shyu, R. Y. Huang, M. F. Tang, H. S. Young, T. H. Roffler, S. R. Chiou, Y. S. Yeh, M. Y. Br J Cancer Research Article The presence of tumour cells in the blood circulation may predict disease recurrence and metastasis. We have evaluated the specificity and sensitivity of detecting hepatoma cells in blood using nested polymerase chain reaction with primers specific for the alphafetoprotein (AFP) gene. The nested polymerase chain reaction amplified a 270-base pair AFP DNA fragment from cDNA of Hep 3B hepatoma cells. In a reconstitution experiment, AFP mRNA was detected from peripheral mononuclear cells isolated from 10 ml of blood containing as few as ten Hep 3B cells. Peripheral mononuclear cells from the blood of 20 hepatoma patients were analysed, and 19 patients showed positive AFP mRNA expression. Seven of 13 samples from hepatitis patients also showed positive AFP mRNA expression. All five paired samples of peripheral blood or umbilical cord blood from pregnant mothers and their babies, respectively, showed positive AFP expression. None of 22 control samples was positive. The presence of AFP mRNA in the blood of hepatitis or hepatoma patients suggests the presence of circulating hepatoma cells or hepatocytes in the circulation. The high incidence of AFP mRNA in the blood of hepatoma patients supports the notion of early haematogenous spreading of the disease. IMAGES: Nature Publishing Group|1 1997 /pmc/articles/PMC2063396/ /pubmed/9062418 Text en https://creativecommons.org/licenses/by/4.0/This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit https://creativecommons.org/licenses/by/4.0/.
spellingShingle Research Article
Jiang, S. Y.
Shyu, R. Y.
Huang, M. F.
Tang, H. S.
Young, T. H.
Roffler, S. R.
Chiou, Y. S.
Yeh, M. Y.
Detection of alphafetoprotein-expressing cells in the blood of patients with hepatoma and hepatitis.
title Detection of alphafetoprotein-expressing cells in the blood of patients with hepatoma and hepatitis.
title_full Detection of alphafetoprotein-expressing cells in the blood of patients with hepatoma and hepatitis.
title_fullStr Detection of alphafetoprotein-expressing cells in the blood of patients with hepatoma and hepatitis.
title_full_unstemmed Detection of alphafetoprotein-expressing cells in the blood of patients with hepatoma and hepatitis.
title_short Detection of alphafetoprotein-expressing cells in the blood of patients with hepatoma and hepatitis.
title_sort detection of alphafetoprotein-expressing cells in the blood of patients with hepatoma and hepatitis.
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063396/
https://www.ncbi.nlm.nih.gov/pubmed/9062418
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