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Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells

The growth and repair of skeletal muscle after birth depends on satellite cells that are characterized by the expression of Pax7. We show that Pax3, the paralogue of Pax7, is also present in both quiescent and activated satellite cells in many skeletal muscles. Dominant-negative forms of both Pax3 a...

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Autores principales: Relaix, Frédéric, Montarras, Didier, Zaffran, Stéphane, Gayraud-Morel, Barbara, Rocancourt, Didier, Tajbakhsh, Shahragim, Mansouri, Ahmed, Cumano, Ana, Buckingham, Margaret
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063537/
https://www.ncbi.nlm.nih.gov/pubmed/16380438
http://dx.doi.org/10.1083/jcb.200508044
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author Relaix, Frédéric
Montarras, Didier
Zaffran, Stéphane
Gayraud-Morel, Barbara
Rocancourt, Didier
Tajbakhsh, Shahragim
Mansouri, Ahmed
Cumano, Ana
Buckingham, Margaret
author_facet Relaix, Frédéric
Montarras, Didier
Zaffran, Stéphane
Gayraud-Morel, Barbara
Rocancourt, Didier
Tajbakhsh, Shahragim
Mansouri, Ahmed
Cumano, Ana
Buckingham, Margaret
author_sort Relaix, Frédéric
collection PubMed
description The growth and repair of skeletal muscle after birth depends on satellite cells that are characterized by the expression of Pax7. We show that Pax3, the paralogue of Pax7, is also present in both quiescent and activated satellite cells in many skeletal muscles. Dominant-negative forms of both Pax3 and -7 repress MyoD, but do not interfere with the expression of the other myogenic determination factor, Myf5, which, together with Pax3/7, regulates the myogenic differentiation of these cells. In Pax7 mutants, satellite cells are progressively lost in both Pax3-expressing and -nonexpressing muscles. We show that this is caused by satellite cell death, with effects on the cell cycle. Manipulation of the dominant-negative forms of these factors in satellite cell cultures demonstrates that Pax3 cannot replace the antiapoptotic function of Pax7. These findings underline the importance of cell survival in controlling the stem cell populations of adult tissues and demonstrate a role for upstream factors in this context.
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spelling pubmed-20635372008-03-19 Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells Relaix, Frédéric Montarras, Didier Zaffran, Stéphane Gayraud-Morel, Barbara Rocancourt, Didier Tajbakhsh, Shahragim Mansouri, Ahmed Cumano, Ana Buckingham, Margaret J Cell Biol Research Articles The growth and repair of skeletal muscle after birth depends on satellite cells that are characterized by the expression of Pax7. We show that Pax3, the paralogue of Pax7, is also present in both quiescent and activated satellite cells in many skeletal muscles. Dominant-negative forms of both Pax3 and -7 repress MyoD, but do not interfere with the expression of the other myogenic determination factor, Myf5, which, together with Pax3/7, regulates the myogenic differentiation of these cells. In Pax7 mutants, satellite cells are progressively lost in both Pax3-expressing and -nonexpressing muscles. We show that this is caused by satellite cell death, with effects on the cell cycle. Manipulation of the dominant-negative forms of these factors in satellite cell cultures demonstrates that Pax3 cannot replace the antiapoptotic function of Pax7. These findings underline the importance of cell survival in controlling the stem cell populations of adult tissues and demonstrate a role for upstream factors in this context. The Rockefeller University Press 2006-01-02 /pmc/articles/PMC2063537/ /pubmed/16380438 http://dx.doi.org/10.1083/jcb.200508044 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Relaix, Frédéric
Montarras, Didier
Zaffran, Stéphane
Gayraud-Morel, Barbara
Rocancourt, Didier
Tajbakhsh, Shahragim
Mansouri, Ahmed
Cumano, Ana
Buckingham, Margaret
Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells
title Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells
title_full Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells
title_fullStr Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells
title_full_unstemmed Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells
title_short Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells
title_sort pax3 and pax7 have distinct and overlapping functions in adult muscle progenitor cells
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063537/
https://www.ncbi.nlm.nih.gov/pubmed/16380438
http://dx.doi.org/10.1083/jcb.200508044
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