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Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells
The growth and repair of skeletal muscle after birth depends on satellite cells that are characterized by the expression of Pax7. We show that Pax3, the paralogue of Pax7, is also present in both quiescent and activated satellite cells in many skeletal muscles. Dominant-negative forms of both Pax3 a...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063537/ https://www.ncbi.nlm.nih.gov/pubmed/16380438 http://dx.doi.org/10.1083/jcb.200508044 |
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author | Relaix, Frédéric Montarras, Didier Zaffran, Stéphane Gayraud-Morel, Barbara Rocancourt, Didier Tajbakhsh, Shahragim Mansouri, Ahmed Cumano, Ana Buckingham, Margaret |
author_facet | Relaix, Frédéric Montarras, Didier Zaffran, Stéphane Gayraud-Morel, Barbara Rocancourt, Didier Tajbakhsh, Shahragim Mansouri, Ahmed Cumano, Ana Buckingham, Margaret |
author_sort | Relaix, Frédéric |
collection | PubMed |
description | The growth and repair of skeletal muscle after birth depends on satellite cells that are characterized by the expression of Pax7. We show that Pax3, the paralogue of Pax7, is also present in both quiescent and activated satellite cells in many skeletal muscles. Dominant-negative forms of both Pax3 and -7 repress MyoD, but do not interfere with the expression of the other myogenic determination factor, Myf5, which, together with Pax3/7, regulates the myogenic differentiation of these cells. In Pax7 mutants, satellite cells are progressively lost in both Pax3-expressing and -nonexpressing muscles. We show that this is caused by satellite cell death, with effects on the cell cycle. Manipulation of the dominant-negative forms of these factors in satellite cell cultures demonstrates that Pax3 cannot replace the antiapoptotic function of Pax7. These findings underline the importance of cell survival in controlling the stem cell populations of adult tissues and demonstrate a role for upstream factors in this context. |
format | Text |
id | pubmed-2063537 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20635372008-03-19 Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells Relaix, Frédéric Montarras, Didier Zaffran, Stéphane Gayraud-Morel, Barbara Rocancourt, Didier Tajbakhsh, Shahragim Mansouri, Ahmed Cumano, Ana Buckingham, Margaret J Cell Biol Research Articles The growth and repair of skeletal muscle after birth depends on satellite cells that are characterized by the expression of Pax7. We show that Pax3, the paralogue of Pax7, is also present in both quiescent and activated satellite cells in many skeletal muscles. Dominant-negative forms of both Pax3 and -7 repress MyoD, but do not interfere with the expression of the other myogenic determination factor, Myf5, which, together with Pax3/7, regulates the myogenic differentiation of these cells. In Pax7 mutants, satellite cells are progressively lost in both Pax3-expressing and -nonexpressing muscles. We show that this is caused by satellite cell death, with effects on the cell cycle. Manipulation of the dominant-negative forms of these factors in satellite cell cultures demonstrates that Pax3 cannot replace the antiapoptotic function of Pax7. These findings underline the importance of cell survival in controlling the stem cell populations of adult tissues and demonstrate a role for upstream factors in this context. The Rockefeller University Press 2006-01-02 /pmc/articles/PMC2063537/ /pubmed/16380438 http://dx.doi.org/10.1083/jcb.200508044 Text en This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Relaix, Frédéric Montarras, Didier Zaffran, Stéphane Gayraud-Morel, Barbara Rocancourt, Didier Tajbakhsh, Shahragim Mansouri, Ahmed Cumano, Ana Buckingham, Margaret Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells |
title | Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells |
title_full | Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells |
title_fullStr | Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells |
title_full_unstemmed | Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells |
title_short | Pax3 and Pax7 have distinct and overlapping functions in adult muscle progenitor cells |
title_sort | pax3 and pax7 have distinct and overlapping functions in adult muscle progenitor cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063537/ https://www.ncbi.nlm.nih.gov/pubmed/16380438 http://dx.doi.org/10.1083/jcb.200508044 |
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