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Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region
In the yeast Saccharomyces cerevisiae that lacks lamins, the nuclear pore complex (NPC) has been proposed to serve a role in chromatin organization. Here, using fluorescence microscopy in living cells, we show that nuclear pore proteins of the Nup84 core complex, Nup84p, Nup145Cp, Nup120p, and Nup13...
Autores principales: | , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063549/ https://www.ncbi.nlm.nih.gov/pubmed/16418532 http://dx.doi.org/10.1083/jcb.200505159 |
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author | Therizols, Pierre Fairhead, Cécile Cabal, Ghislain G. Genovesio, Auguste Olivo-Marin, Jean-Christophe Dujon, Bernard Fabre, Emmanuelle |
author_facet | Therizols, Pierre Fairhead, Cécile Cabal, Ghislain G. Genovesio, Auguste Olivo-Marin, Jean-Christophe Dujon, Bernard Fabre, Emmanuelle |
author_sort | Therizols, Pierre |
collection | PubMed |
description | In the yeast Saccharomyces cerevisiae that lacks lamins, the nuclear pore complex (NPC) has been proposed to serve a role in chromatin organization. Here, using fluorescence microscopy in living cells, we show that nuclear pore proteins of the Nup84 core complex, Nup84p, Nup145Cp, Nup120p, and Nup133p, serve to anchor telomere XI-L at the nuclear periphery. The integrity of this complex is shown to be required for repression of a URA3 gene inserted in the subtelomeric region of this chromosome end. Furthermore, altering the integrity of this complex decreases the efficiency of repair of a DNA double-strand break (DSB) only when it is generated in the subtelomeric region, even though the repair machinery is functional. These effects are specific to the Nup84 complex. Our observations thus confirm and extend the role played by the NPC, through the Nup84 complex, in the functional organization of chromatin. They also indicate that anchoring of telomeres is essential for efficient repair of DSBs occurring therein and is important for preserving genome integrity. |
format | Text |
id | pubmed-2063549 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20635492008-03-19 Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region Therizols, Pierre Fairhead, Cécile Cabal, Ghislain G. Genovesio, Auguste Olivo-Marin, Jean-Christophe Dujon, Bernard Fabre, Emmanuelle J Cell Biol Research Articles In the yeast Saccharomyces cerevisiae that lacks lamins, the nuclear pore complex (NPC) has been proposed to serve a role in chromatin organization. Here, using fluorescence microscopy in living cells, we show that nuclear pore proteins of the Nup84 core complex, Nup84p, Nup145Cp, Nup120p, and Nup133p, serve to anchor telomere XI-L at the nuclear periphery. The integrity of this complex is shown to be required for repression of a URA3 gene inserted in the subtelomeric region of this chromosome end. Furthermore, altering the integrity of this complex decreases the efficiency of repair of a DNA double-strand break (DSB) only when it is generated in the subtelomeric region, even though the repair machinery is functional. These effects are specific to the Nup84 complex. Our observations thus confirm and extend the role played by the NPC, through the Nup84 complex, in the functional organization of chromatin. They also indicate that anchoring of telomeres is essential for efficient repair of DSBs occurring therein and is important for preserving genome integrity. The Rockefeller University Press 2006-01-16 /pmc/articles/PMC2063549/ /pubmed/16418532 http://dx.doi.org/10.1083/jcb.200505159 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Therizols, Pierre Fairhead, Cécile Cabal, Ghislain G. Genovesio, Auguste Olivo-Marin, Jean-Christophe Dujon, Bernard Fabre, Emmanuelle Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region |
title | Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region |
title_full | Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region |
title_fullStr | Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region |
title_full_unstemmed | Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region |
title_short | Telomere tethering at the nuclear periphery is essential for efficient DNA double strand break repair in subtelomeric region |
title_sort | telomere tethering at the nuclear periphery is essential for efficient dna double strand break repair in subtelomeric region |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063549/ https://www.ncbi.nlm.nih.gov/pubmed/16418532 http://dx.doi.org/10.1083/jcb.200505159 |
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