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The brain-specific double-stranded RNA-binding protein Staufen2 is required for dendritic spine morphogenesis

Mammalian Staufen2 (Stau2) is a member of the double-stranded RNA-binding protein family. Its expression is largely restricted to the brain. It is thought to play a role in the delivery of RNA to dendrites of polarized neurons. To investigate the function of Stau2 in mature neurons, we interfered wi...

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Autores principales: Goetze, Bernhard, Tuebing, Fabian, Xie, Yunli, Dorostkar, Mario M., Thomas, Sabine, Pehl, Ulrich, Boehm, Stefan, Macchi, Paolo, Kiebler, Michael A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063552/
https://www.ncbi.nlm.nih.gov/pubmed/16418534
http://dx.doi.org/10.1083/jcb.200509035
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author Goetze, Bernhard
Tuebing, Fabian
Xie, Yunli
Dorostkar, Mario M.
Thomas, Sabine
Pehl, Ulrich
Boehm, Stefan
Macchi, Paolo
Kiebler, Michael A.
author_facet Goetze, Bernhard
Tuebing, Fabian
Xie, Yunli
Dorostkar, Mario M.
Thomas, Sabine
Pehl, Ulrich
Boehm, Stefan
Macchi, Paolo
Kiebler, Michael A.
author_sort Goetze, Bernhard
collection PubMed
description Mammalian Staufen2 (Stau2) is a member of the double-stranded RNA-binding protein family. Its expression is largely restricted to the brain. It is thought to play a role in the delivery of RNA to dendrites of polarized neurons. To investigate the function of Stau2 in mature neurons, we interfered with Stau2 expression by RNA interference (RNAi). Mature neurons lacking Stau2 displayed a significant reduction in the number of dendritic spines and an increase in filopodia-like structures. The number of PSD95-positive synapses and miniature excitatory postsynaptic currents were markedly reduced in Stau2 down-regulated neurons. Akin effects were caused by overexpression of dominant-negative Stau2. The observed phenotype could be rescued by overexpression of two RNAi cleavage-resistant Stau2 isoforms. In situ hybridization revealed reduced expression levels of β-actin mRNA and fewer dendritic β-actin mRNPs in Stau2 down-regulated neurons. Thus, our data suggest an important role for Stau2 in the formation and maintenance of dendritic spines of hippocampal neurons.
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spelling pubmed-20635522008-03-19 The brain-specific double-stranded RNA-binding protein Staufen2 is required for dendritic spine morphogenesis Goetze, Bernhard Tuebing, Fabian Xie, Yunli Dorostkar, Mario M. Thomas, Sabine Pehl, Ulrich Boehm, Stefan Macchi, Paolo Kiebler, Michael A. J Cell Biol Research Articles Mammalian Staufen2 (Stau2) is a member of the double-stranded RNA-binding protein family. Its expression is largely restricted to the brain. It is thought to play a role in the delivery of RNA to dendrites of polarized neurons. To investigate the function of Stau2 in mature neurons, we interfered with Stau2 expression by RNA interference (RNAi). Mature neurons lacking Stau2 displayed a significant reduction in the number of dendritic spines and an increase in filopodia-like structures. The number of PSD95-positive synapses and miniature excitatory postsynaptic currents were markedly reduced in Stau2 down-regulated neurons. Akin effects were caused by overexpression of dominant-negative Stau2. The observed phenotype could be rescued by overexpression of two RNAi cleavage-resistant Stau2 isoforms. In situ hybridization revealed reduced expression levels of β-actin mRNA and fewer dendritic β-actin mRNPs in Stau2 down-regulated neurons. Thus, our data suggest an important role for Stau2 in the formation and maintenance of dendritic spines of hippocampal neurons. The Rockefeller University Press 2006-01-16 /pmc/articles/PMC2063552/ /pubmed/16418534 http://dx.doi.org/10.1083/jcb.200509035 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Goetze, Bernhard
Tuebing, Fabian
Xie, Yunli
Dorostkar, Mario M.
Thomas, Sabine
Pehl, Ulrich
Boehm, Stefan
Macchi, Paolo
Kiebler, Michael A.
The brain-specific double-stranded RNA-binding protein Staufen2 is required for dendritic spine morphogenesis
title The brain-specific double-stranded RNA-binding protein Staufen2 is required for dendritic spine morphogenesis
title_full The brain-specific double-stranded RNA-binding protein Staufen2 is required for dendritic spine morphogenesis
title_fullStr The brain-specific double-stranded RNA-binding protein Staufen2 is required for dendritic spine morphogenesis
title_full_unstemmed The brain-specific double-stranded RNA-binding protein Staufen2 is required for dendritic spine morphogenesis
title_short The brain-specific double-stranded RNA-binding protein Staufen2 is required for dendritic spine morphogenesis
title_sort brain-specific double-stranded rna-binding protein staufen2 is required for dendritic spine morphogenesis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063552/
https://www.ncbi.nlm.nih.gov/pubmed/16418534
http://dx.doi.org/10.1083/jcb.200509035
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