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Structural transitions in the synaptic SNARE complex during Ca(2+)-triggered exocytosis
The synaptic SNARE complex is a highly stable four-helix bundle that links the vesicle and plasma membranes and plays an essential role in the Ca(2+)-triggered release of neurotransmitters and hormones. An understanding has yet to be achieved of how this complex assembles and undergoes structural tr...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063557/ https://www.ncbi.nlm.nih.gov/pubmed/16418536 http://dx.doi.org/10.1083/jcb.200510012 |
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author | Han, Xue Jackson, Meyer B. |
author_facet | Han, Xue Jackson, Meyer B. |
author_sort | Han, Xue |
collection | PubMed |
description | The synaptic SNARE complex is a highly stable four-helix bundle that links the vesicle and plasma membranes and plays an essential role in the Ca(2+)-triggered release of neurotransmitters and hormones. An understanding has yet to be achieved of how this complex assembles and undergoes structural transitions during exocytosis. To investigate this question, we have mutated residues within the hydrophobic core of the SNARE complex along the entire length of all four chains and examined the consequences using amperometry to measure fusion pore opening and dilation. Mutations throughout the SNARE complex reduced two distinct rate processes before fusion pore opening to different degrees. These results suggest that two distinct, fully assembled conformations of the SNARE complex drive transitions leading to open fusion pores. In contrast, a smaller number of mutations that were scattered through the SNARE complex but were somewhat concentrated in the membrane-distal half stabilized open fusion pores. These results suggest that a structural transition within a partially disassembled complex drives the dilation of open fusion pores. The dependence of these three rate processes on position within the SNARE complex does not support vectorial SNARE complex zipping during exocytosis. |
format | Text |
id | pubmed-2063557 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20635572008-03-19 Structural transitions in the synaptic SNARE complex during Ca(2+)-triggered exocytosis Han, Xue Jackson, Meyer B. J Cell Biol Research Articles The synaptic SNARE complex is a highly stable four-helix bundle that links the vesicle and plasma membranes and plays an essential role in the Ca(2+)-triggered release of neurotransmitters and hormones. An understanding has yet to be achieved of how this complex assembles and undergoes structural transitions during exocytosis. To investigate this question, we have mutated residues within the hydrophobic core of the SNARE complex along the entire length of all four chains and examined the consequences using amperometry to measure fusion pore opening and dilation. Mutations throughout the SNARE complex reduced two distinct rate processes before fusion pore opening to different degrees. These results suggest that two distinct, fully assembled conformations of the SNARE complex drive transitions leading to open fusion pores. In contrast, a smaller number of mutations that were scattered through the SNARE complex but were somewhat concentrated in the membrane-distal half stabilized open fusion pores. These results suggest that a structural transition within a partially disassembled complex drives the dilation of open fusion pores. The dependence of these three rate processes on position within the SNARE complex does not support vectorial SNARE complex zipping during exocytosis. The Rockefeller University Press 2006-01-16 /pmc/articles/PMC2063557/ /pubmed/16418536 http://dx.doi.org/10.1083/jcb.200510012 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Han, Xue Jackson, Meyer B. Structural transitions in the synaptic SNARE complex during Ca(2+)-triggered exocytosis |
title | Structural transitions in the synaptic SNARE complex during Ca(2+)-triggered exocytosis |
title_full | Structural transitions in the synaptic SNARE complex during Ca(2+)-triggered exocytosis |
title_fullStr | Structural transitions in the synaptic SNARE complex during Ca(2+)-triggered exocytosis |
title_full_unstemmed | Structural transitions in the synaptic SNARE complex during Ca(2+)-triggered exocytosis |
title_short | Structural transitions in the synaptic SNARE complex during Ca(2+)-triggered exocytosis |
title_sort | structural transitions in the synaptic snare complex during ca(2+)-triggered exocytosis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063557/ https://www.ncbi.nlm.nih.gov/pubmed/16418536 http://dx.doi.org/10.1083/jcb.200510012 |
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