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Two distinct modes of myosin assembly and dynamics during epithelial wound closure

Actomyosin contraction powers the sealing of epithelial sheets during embryogenesis and wound closure; however, the mechanisms are poorly understood. After laser ablation wounding of Madin–Darby canine kidney cell monolayers, we observed distinct steps in wound closure from time-lapse images of myos...

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Detalles Bibliográficos
Autores principales: Tamada, Masako, Perez, Tomas D., Nelson, W. James, Sheetz, Michael P.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063619/
https://www.ncbi.nlm.nih.gov/pubmed/17200415
http://dx.doi.org/10.1083/jcb.200609116
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author Tamada, Masako
Perez, Tomas D.
Nelson, W. James
Sheetz, Michael P.
author_facet Tamada, Masako
Perez, Tomas D.
Nelson, W. James
Sheetz, Michael P.
author_sort Tamada, Masako
collection PubMed
description Actomyosin contraction powers the sealing of epithelial sheets during embryogenesis and wound closure; however, the mechanisms are poorly understood. After laser ablation wounding of Madin–Darby canine kidney cell monolayers, we observed distinct steps in wound closure from time-lapse images of myosin distribution during resealing. Immediately upon wounding, actin and myosin II regulatory light chain accumulated at two locations: (1) in a ring adjacent to the tight junction that circumscribed the wound and (2) in fibers at the base of the cell in membranes extending over the wound site. Rho-kinase activity was required for assembly of the myosin ring, and myosin II activity was required for contraction but not for basal membrane extension. As it contracted, the myosin ring moved toward the basal membrane with ZO-1 and Rho-kinase. Thus, we suggest that tight junctions serve as attachment points for the actomyosin ring during wound closure and that Rho-kinase is required for localization and activation of the contractile ring.
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spelling pubmed-20636192007-11-29 Two distinct modes of myosin assembly and dynamics during epithelial wound closure Tamada, Masako Perez, Tomas D. Nelson, W. James Sheetz, Michael P. J Cell Biol Research Articles Actomyosin contraction powers the sealing of epithelial sheets during embryogenesis and wound closure; however, the mechanisms are poorly understood. After laser ablation wounding of Madin–Darby canine kidney cell monolayers, we observed distinct steps in wound closure from time-lapse images of myosin distribution during resealing. Immediately upon wounding, actin and myosin II regulatory light chain accumulated at two locations: (1) in a ring adjacent to the tight junction that circumscribed the wound and (2) in fibers at the base of the cell in membranes extending over the wound site. Rho-kinase activity was required for assembly of the myosin ring, and myosin II activity was required for contraction but not for basal membrane extension. As it contracted, the myosin ring moved toward the basal membrane with ZO-1 and Rho-kinase. Thus, we suggest that tight junctions serve as attachment points for the actomyosin ring during wound closure and that Rho-kinase is required for localization and activation of the contractile ring. The Rockefeller University Press 2007-01-01 /pmc/articles/PMC2063619/ /pubmed/17200415 http://dx.doi.org/10.1083/jcb.200609116 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Tamada, Masako
Perez, Tomas D.
Nelson, W. James
Sheetz, Michael P.
Two distinct modes of myosin assembly and dynamics during epithelial wound closure
title Two distinct modes of myosin assembly and dynamics during epithelial wound closure
title_full Two distinct modes of myosin assembly and dynamics during epithelial wound closure
title_fullStr Two distinct modes of myosin assembly and dynamics during epithelial wound closure
title_full_unstemmed Two distinct modes of myosin assembly and dynamics during epithelial wound closure
title_short Two distinct modes of myosin assembly and dynamics during epithelial wound closure
title_sort two distinct modes of myosin assembly and dynamics during epithelial wound closure
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063619/
https://www.ncbi.nlm.nih.gov/pubmed/17200415
http://dx.doi.org/10.1083/jcb.200609116
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