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Two distinct modes of myosin assembly and dynamics during epithelial wound closure
Actomyosin contraction powers the sealing of epithelial sheets during embryogenesis and wound closure; however, the mechanisms are poorly understood. After laser ablation wounding of Madin–Darby canine kidney cell monolayers, we observed distinct steps in wound closure from time-lapse images of myos...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063619/ https://www.ncbi.nlm.nih.gov/pubmed/17200415 http://dx.doi.org/10.1083/jcb.200609116 |
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author | Tamada, Masako Perez, Tomas D. Nelson, W. James Sheetz, Michael P. |
author_facet | Tamada, Masako Perez, Tomas D. Nelson, W. James Sheetz, Michael P. |
author_sort | Tamada, Masako |
collection | PubMed |
description | Actomyosin contraction powers the sealing of epithelial sheets during embryogenesis and wound closure; however, the mechanisms are poorly understood. After laser ablation wounding of Madin–Darby canine kidney cell monolayers, we observed distinct steps in wound closure from time-lapse images of myosin distribution during resealing. Immediately upon wounding, actin and myosin II regulatory light chain accumulated at two locations: (1) in a ring adjacent to the tight junction that circumscribed the wound and (2) in fibers at the base of the cell in membranes extending over the wound site. Rho-kinase activity was required for assembly of the myosin ring, and myosin II activity was required for contraction but not for basal membrane extension. As it contracted, the myosin ring moved toward the basal membrane with ZO-1 and Rho-kinase. Thus, we suggest that tight junctions serve as attachment points for the actomyosin ring during wound closure and that Rho-kinase is required for localization and activation of the contractile ring. |
format | Text |
id | pubmed-2063619 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20636192007-11-29 Two distinct modes of myosin assembly and dynamics during epithelial wound closure Tamada, Masako Perez, Tomas D. Nelson, W. James Sheetz, Michael P. J Cell Biol Research Articles Actomyosin contraction powers the sealing of epithelial sheets during embryogenesis and wound closure; however, the mechanisms are poorly understood. After laser ablation wounding of Madin–Darby canine kidney cell monolayers, we observed distinct steps in wound closure from time-lapse images of myosin distribution during resealing. Immediately upon wounding, actin and myosin II regulatory light chain accumulated at two locations: (1) in a ring adjacent to the tight junction that circumscribed the wound and (2) in fibers at the base of the cell in membranes extending over the wound site. Rho-kinase activity was required for assembly of the myosin ring, and myosin II activity was required for contraction but not for basal membrane extension. As it contracted, the myosin ring moved toward the basal membrane with ZO-1 and Rho-kinase. Thus, we suggest that tight junctions serve as attachment points for the actomyosin ring during wound closure and that Rho-kinase is required for localization and activation of the contractile ring. The Rockefeller University Press 2007-01-01 /pmc/articles/PMC2063619/ /pubmed/17200415 http://dx.doi.org/10.1083/jcb.200609116 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Tamada, Masako Perez, Tomas D. Nelson, W. James Sheetz, Michael P. Two distinct modes of myosin assembly and dynamics during epithelial wound closure |
title | Two distinct modes of myosin assembly and dynamics during epithelial wound closure |
title_full | Two distinct modes of myosin assembly and dynamics during epithelial wound closure |
title_fullStr | Two distinct modes of myosin assembly and dynamics during epithelial wound closure |
title_full_unstemmed | Two distinct modes of myosin assembly and dynamics during epithelial wound closure |
title_short | Two distinct modes of myosin assembly and dynamics during epithelial wound closure |
title_sort | two distinct modes of myosin assembly and dynamics during epithelial wound closure |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063619/ https://www.ncbi.nlm.nih.gov/pubmed/17200415 http://dx.doi.org/10.1083/jcb.200609116 |
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