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KIF14 and citron kinase act together to promote efficient cytokinesis

Multiple mitotic kinesins and microtubule-associated proteins (MAPs) act in concert to direct cytokinesis (Glotzer, M. 2005. Science. 307:1735–1739). In anaphase cells, many of these proteins associate with an antiparallel array of microtubules termed the central spindle. The MAP and microtubule-bun...

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Autores principales: Gruneberg, Ulrike, Neef, Rüdiger, Li, Xiuling, Chan, Eunice H.Y., Chalamalasetty, Ravindra B., Nigg, Erich A., Barr, Francis A.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063646/
https://www.ncbi.nlm.nih.gov/pubmed/16431929
http://dx.doi.org/10.1083/jcb.200511061
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author Gruneberg, Ulrike
Neef, Rüdiger
Li, Xiuling
Chan, Eunice H.Y.
Chalamalasetty, Ravindra B.
Nigg, Erich A.
Barr, Francis A.
author_facet Gruneberg, Ulrike
Neef, Rüdiger
Li, Xiuling
Chan, Eunice H.Y.
Chalamalasetty, Ravindra B.
Nigg, Erich A.
Barr, Francis A.
author_sort Gruneberg, Ulrike
collection PubMed
description Multiple mitotic kinesins and microtubule-associated proteins (MAPs) act in concert to direct cytokinesis (Glotzer, M. 2005. Science. 307:1735–1739). In anaphase cells, many of these proteins associate with an antiparallel array of microtubules termed the central spindle. The MAP and microtubule-bundling protein PRC1 (protein-regulating cytokinesis 1) is one of the key molecules required for the integrity of this structure (Jiang, W., G. Jimenez, N.J. Wells, T.J. Hope, G.M. Wahl, T. Hunter, and R. Fukunaga. 1998. Mol. Cell. 2:877–885; Mollinari, C., J.P. Kleman, W. Jiang, G. Schoehn, T. Hunter, and R.L. Margolis. 2002. J. Cell Biol. 157:1175–1186). In this study, we identify an interaction between endogenous PRC1 and the previously uncharacterized kinesin KIF14 as well as other mitotic kinesins (MKlp1/CHO1, MKlp2, and KIF4) with known functions in cytokinesis (Hill, E., M. Clarke, and F.A. Barr. 2000. EMBO J. 19:5711–5719; Matuliene, J., and R. Kuriyama. 2002. Mol. Biol. Cell. 13:1832–1845; Kurasawa, Y., W.C. Earnshaw, Y. Mochizuki, N. Dohmae, and K. Todokoro. 2004. EMBO J. 23:3237–3248). We find that KIF14 targets to the central spindle via its interaction with PRC1 and has an essential function in cytokinesis. In KIF14-depleted cells, citron kinase but not other components of the central spindle and cleavage furrow fail to localize. Furthermore, the localization of KIF14 and citron kinase to the central spindle and midbody is codependent, and they form a complex depending on the activation state of citron kinase. Contrary to a previous study (Di Cunto, F., S. Imarisio, E. Hirsch, V. Broccoli, A. Bulfone, A. Migheli, C. Atzori, E. Turco, R. Triolo, G.P. Dotto, et al. 2000. Neuron. 28:115–127), we find a general requirement for citron kinase in human cell division. Together, these findings identify a novel pathway required for efficient cytokinesis.
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spelling pubmed-20636462007-11-29 KIF14 and citron kinase act together to promote efficient cytokinesis Gruneberg, Ulrike Neef, Rüdiger Li, Xiuling Chan, Eunice H.Y. Chalamalasetty, Ravindra B. Nigg, Erich A. Barr, Francis A. J Cell Biol Research Articles Multiple mitotic kinesins and microtubule-associated proteins (MAPs) act in concert to direct cytokinesis (Glotzer, M. 2005. Science. 307:1735–1739). In anaphase cells, many of these proteins associate with an antiparallel array of microtubules termed the central spindle. The MAP and microtubule-bundling protein PRC1 (protein-regulating cytokinesis 1) is one of the key molecules required for the integrity of this structure (Jiang, W., G. Jimenez, N.J. Wells, T.J. Hope, G.M. Wahl, T. Hunter, and R. Fukunaga. 1998. Mol. Cell. 2:877–885; Mollinari, C., J.P. Kleman, W. Jiang, G. Schoehn, T. Hunter, and R.L. Margolis. 2002. J. Cell Biol. 157:1175–1186). In this study, we identify an interaction between endogenous PRC1 and the previously uncharacterized kinesin KIF14 as well as other mitotic kinesins (MKlp1/CHO1, MKlp2, and KIF4) with known functions in cytokinesis (Hill, E., M. Clarke, and F.A. Barr. 2000. EMBO J. 19:5711–5719; Matuliene, J., and R. Kuriyama. 2002. Mol. Biol. Cell. 13:1832–1845; Kurasawa, Y., W.C. Earnshaw, Y. Mochizuki, N. Dohmae, and K. Todokoro. 2004. EMBO J. 23:3237–3248). We find that KIF14 targets to the central spindle via its interaction with PRC1 and has an essential function in cytokinesis. In KIF14-depleted cells, citron kinase but not other components of the central spindle and cleavage furrow fail to localize. Furthermore, the localization of KIF14 and citron kinase to the central spindle and midbody is codependent, and they form a complex depending on the activation state of citron kinase. Contrary to a previous study (Di Cunto, F., S. Imarisio, E. Hirsch, V. Broccoli, A. Bulfone, A. Migheli, C. Atzori, E. Turco, R. Triolo, G.P. Dotto, et al. 2000. Neuron. 28:115–127), we find a general requirement for citron kinase in human cell division. Together, these findings identify a novel pathway required for efficient cytokinesis. The Rockefeller University Press 2006-01-30 /pmc/articles/PMC2063646/ /pubmed/16431929 http://dx.doi.org/10.1083/jcb.200511061 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Gruneberg, Ulrike
Neef, Rüdiger
Li, Xiuling
Chan, Eunice H.Y.
Chalamalasetty, Ravindra B.
Nigg, Erich A.
Barr, Francis A.
KIF14 and citron kinase act together to promote efficient cytokinesis
title KIF14 and citron kinase act together to promote efficient cytokinesis
title_full KIF14 and citron kinase act together to promote efficient cytokinesis
title_fullStr KIF14 and citron kinase act together to promote efficient cytokinesis
title_full_unstemmed KIF14 and citron kinase act together to promote efficient cytokinesis
title_short KIF14 and citron kinase act together to promote efficient cytokinesis
title_sort kif14 and citron kinase act together to promote efficient cytokinesis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063646/
https://www.ncbi.nlm.nih.gov/pubmed/16431929
http://dx.doi.org/10.1083/jcb.200511061
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