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ERK1c regulates Golgi fragmentation during mitosis
Extracellular signal-regulated kinase 1c (ERK1c) is an alternatively spliced form of ERK1 that is regulated differently than other ERK isoforms. We studied the Golgi functions of ERK1c and found that it plays a role in MEK-induced mitotic Golgi fragmentation. Thus, in late G2 and mitosis of synchron...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063732/ https://www.ncbi.nlm.nih.gov/pubmed/16533948 http://dx.doi.org/10.1083/jcb.200509063 |
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author | Shaul, Yoav D. Seger, Rony |
author_facet | Shaul, Yoav D. Seger, Rony |
author_sort | Shaul, Yoav D. |
collection | PubMed |
description | Extracellular signal-regulated kinase 1c (ERK1c) is an alternatively spliced form of ERK1 that is regulated differently than other ERK isoforms. We studied the Golgi functions of ERK1c and found that it plays a role in MEK-induced mitotic Golgi fragmentation. Thus, in late G2 and mitosis of synchronized cells, the expression and activity of ERK1c was increased and it colocalized mainly with Golgi markers. Small interfering RNA of ERK1c significantly attenuated, whereas ERK1c overexpression facilitated, mitotic Golgi fragmentation. These effects were also reflected in mitotic progression, indicating that ERK1c is involved in cell cycle regulation via modulation of Golgi fragmentation. Although ERK1 was activated in mitosis as well, it could not replace ERK1c in regulating Golgi fragmentation. Therefore, MEKs regulate mitosis via all three ERK isoforms, where ERK1c acts specifically in the Golgi, whereas ERK1 and 2 regulate other mitosis-related processes. Thus, ERK1c extends the specificity of the Ras-MEK cascade by activating ERK1/2-independent processes. |
format | Text |
id | pubmed-2063732 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20637322007-11-29 ERK1c regulates Golgi fragmentation during mitosis Shaul, Yoav D. Seger, Rony J Cell Biol Research Articles Extracellular signal-regulated kinase 1c (ERK1c) is an alternatively spliced form of ERK1 that is regulated differently than other ERK isoforms. We studied the Golgi functions of ERK1c and found that it plays a role in MEK-induced mitotic Golgi fragmentation. Thus, in late G2 and mitosis of synchronized cells, the expression and activity of ERK1c was increased and it colocalized mainly with Golgi markers. Small interfering RNA of ERK1c significantly attenuated, whereas ERK1c overexpression facilitated, mitotic Golgi fragmentation. These effects were also reflected in mitotic progression, indicating that ERK1c is involved in cell cycle regulation via modulation of Golgi fragmentation. Although ERK1 was activated in mitosis as well, it could not replace ERK1c in regulating Golgi fragmentation. Therefore, MEKs regulate mitosis via all three ERK isoforms, where ERK1c acts specifically in the Golgi, whereas ERK1 and 2 regulate other mitosis-related processes. Thus, ERK1c extends the specificity of the Ras-MEK cascade by activating ERK1/2-independent processes. The Rockefeller University Press 2006-03-13 /pmc/articles/PMC2063732/ /pubmed/16533948 http://dx.doi.org/10.1083/jcb.200509063 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Shaul, Yoav D. Seger, Rony ERK1c regulates Golgi fragmentation during mitosis |
title | ERK1c regulates Golgi fragmentation during mitosis |
title_full | ERK1c regulates Golgi fragmentation during mitosis |
title_fullStr | ERK1c regulates Golgi fragmentation during mitosis |
title_full_unstemmed | ERK1c regulates Golgi fragmentation during mitosis |
title_short | ERK1c regulates Golgi fragmentation during mitosis |
title_sort | erk1c regulates golgi fragmentation during mitosis |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063732/ https://www.ncbi.nlm.nih.gov/pubmed/16533948 http://dx.doi.org/10.1083/jcb.200509063 |
work_keys_str_mv | AT shaulyoavd erk1cregulatesgolgifragmentationduringmitosis AT segerrony erk1cregulatesgolgifragmentationduringmitosis |