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NDC1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes

POM121 and gp210 were, until this point, the only known membrane-integral nucleoporins (Nups) of vertebrates and, thus, the only candidate anchors for nuclear pore complexes (NPCs) within the nuclear membrane. In an accompanying study (see Stavru et al. on p. 477 of this issue), we provided evidence...

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Autores principales: Stavru, Fabrizia, Hülsmann, Bastian B., Spang, Anne, Hartmann, Enno, Cordes, Volker C., Görlich, Dirk
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063861/
https://www.ncbi.nlm.nih.gov/pubmed/16702233
http://dx.doi.org/10.1083/jcb.200601001
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author Stavru, Fabrizia
Hülsmann, Bastian B.
Spang, Anne
Hartmann, Enno
Cordes, Volker C.
Görlich, Dirk
author_facet Stavru, Fabrizia
Hülsmann, Bastian B.
Spang, Anne
Hartmann, Enno
Cordes, Volker C.
Görlich, Dirk
author_sort Stavru, Fabrizia
collection PubMed
description POM121 and gp210 were, until this point, the only known membrane-integral nucleoporins (Nups) of vertebrates and, thus, the only candidate anchors for nuclear pore complexes (NPCs) within the nuclear membrane. In an accompanying study (see Stavru et al. on p. 477 of this issue), we provided evidence that NPCs can exist independently of POM121 and gp210, and we predicted that vertebrate NPCs contain additional membrane-integral constituents. We identify such an additional membrane protein in the NPCs of mammals, frogs, insects, and nematodes as the orthologue to yeast Ndc1p/Cut11p. Human NDC1 (hNDC1) likely possesses six transmembrane segments, and it is located at the nuclear pore wall. Depletion of hNDC1 from human HeLa cells interferes with the assembly of phenylalanine-glycine repeat Nups into NPCs. The loss of NDC1 function in Caenorhabditis elegans also causes severe NPC defects and very high larval and embryonic mortality. However, it is not ultimately lethal. Instead, homozygous NDC1-deficient worms can be propagated. This indicates that none of the membrane-integral Nups is universally essential for NPC assembly, and suggests that NPC biogenesis is an extremely fault-tolerant process.
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spelling pubmed-20638612007-11-29 NDC1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes Stavru, Fabrizia Hülsmann, Bastian B. Spang, Anne Hartmann, Enno Cordes, Volker C. Görlich, Dirk J Cell Biol Research Articles POM121 and gp210 were, until this point, the only known membrane-integral nucleoporins (Nups) of vertebrates and, thus, the only candidate anchors for nuclear pore complexes (NPCs) within the nuclear membrane. In an accompanying study (see Stavru et al. on p. 477 of this issue), we provided evidence that NPCs can exist independently of POM121 and gp210, and we predicted that vertebrate NPCs contain additional membrane-integral constituents. We identify such an additional membrane protein in the NPCs of mammals, frogs, insects, and nematodes as the orthologue to yeast Ndc1p/Cut11p. Human NDC1 (hNDC1) likely possesses six transmembrane segments, and it is located at the nuclear pore wall. Depletion of hNDC1 from human HeLa cells interferes with the assembly of phenylalanine-glycine repeat Nups into NPCs. The loss of NDC1 function in Caenorhabditis elegans also causes severe NPC defects and very high larval and embryonic mortality. However, it is not ultimately lethal. Instead, homozygous NDC1-deficient worms can be propagated. This indicates that none of the membrane-integral Nups is universally essential for NPC assembly, and suggests that NPC biogenesis is an extremely fault-tolerant process. The Rockefeller University Press 2006-05-22 /pmc/articles/PMC2063861/ /pubmed/16702233 http://dx.doi.org/10.1083/jcb.200601001 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Stavru, Fabrizia
Hülsmann, Bastian B.
Spang, Anne
Hartmann, Enno
Cordes, Volker C.
Görlich, Dirk
NDC1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes
title NDC1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes
title_full NDC1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes
title_fullStr NDC1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes
title_full_unstemmed NDC1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes
title_short NDC1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes
title_sort ndc1: a crucial membrane-integral nucleoporin of metazoan nuclear pore complexes
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063861/
https://www.ncbi.nlm.nih.gov/pubmed/16702233
http://dx.doi.org/10.1083/jcb.200601001
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