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Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent
Mitochondria are distributed within cells to match local energy demands. We report that the microtubule-dependent transport of mitochondria depends on the ability of milton to act as an adaptor protein that can recruit the heavy chain of conventional kinesin-1 (kinesin heavy chain [KHC]) to mitochon...
Autores principales: | , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2006
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063864/ https://www.ncbi.nlm.nih.gov/pubmed/16717129 http://dx.doi.org/10.1083/jcb.200601067 |
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author | Glater, Elizabeth E. Megeath, Laura J. Stowers, R. Steven Schwarz, Thomas L. |
author_facet | Glater, Elizabeth E. Megeath, Laura J. Stowers, R. Steven Schwarz, Thomas L. |
author_sort | Glater, Elizabeth E. |
collection | PubMed |
description | Mitochondria are distributed within cells to match local energy demands. We report that the microtubule-dependent transport of mitochondria depends on the ability of milton to act as an adaptor protein that can recruit the heavy chain of conventional kinesin-1 (kinesin heavy chain [KHC]) to mitochondria. Biochemical and genetic evidence demonstrate that kinesin recruitment and mitochondrial transport are independent of kinesin light chain (KLC); KLC antagonizes milton's association with KHC and is absent from milton–KHC complexes, and mitochondria are present in klc (−/−) photoreceptor axons. The recruitment of KHC to mitochondria is, in part, determined by the NH(2) terminus–splicing variant of milton. A direct interaction occurs between milton and miro, which is a mitochondrial Rho-like GTPase, and this interaction can influence the recruitment of milton to mitochondria. Thus, milton and miro are likely to form an essential protein complex that links KHC to mitochondria for light chain–independent, anterograde transport of mitochondria. |
format | Text |
id | pubmed-2063864 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20638642007-11-29 Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent Glater, Elizabeth E. Megeath, Laura J. Stowers, R. Steven Schwarz, Thomas L. J Cell Biol Research Articles Mitochondria are distributed within cells to match local energy demands. We report that the microtubule-dependent transport of mitochondria depends on the ability of milton to act as an adaptor protein that can recruit the heavy chain of conventional kinesin-1 (kinesin heavy chain [KHC]) to mitochondria. Biochemical and genetic evidence demonstrate that kinesin recruitment and mitochondrial transport are independent of kinesin light chain (KLC); KLC antagonizes milton's association with KHC and is absent from milton–KHC complexes, and mitochondria are present in klc (−/−) photoreceptor axons. The recruitment of KHC to mitochondria is, in part, determined by the NH(2) terminus–splicing variant of milton. A direct interaction occurs between milton and miro, which is a mitochondrial Rho-like GTPase, and this interaction can influence the recruitment of milton to mitochondria. Thus, milton and miro are likely to form an essential protein complex that links KHC to mitochondria for light chain–independent, anterograde transport of mitochondria. The Rockefeller University Press 2006-05-22 /pmc/articles/PMC2063864/ /pubmed/16717129 http://dx.doi.org/10.1083/jcb.200601067 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Glater, Elizabeth E. Megeath, Laura J. Stowers, R. Steven Schwarz, Thomas L. Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent |
title | Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent |
title_full | Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent |
title_fullStr | Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent |
title_full_unstemmed | Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent |
title_short | Axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent |
title_sort | axonal transport of mitochondria requires milton to recruit kinesin heavy chain and is light chain independent |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063864/ https://www.ncbi.nlm.nih.gov/pubmed/16717129 http://dx.doi.org/10.1083/jcb.200601067 |
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