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Desmoplakin: an unexpected regulator of microtubule organization in the epidermis

Despite their importance in cell shape and polarity generation, the organization of microtubules in differentiated cells and tissues remains relatively unexplored in mammals. We generated transgenic mice in which the epidermis expresses a fluorescently labeled microtubule-binding protein and show th...

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Detalles Bibliográficos
Autores principales: Lechler, Terry, Fuchs, Elaine
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063934/
https://www.ncbi.nlm.nih.gov/pubmed/17227889
http://dx.doi.org/10.1083/jcb.200609109
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author Lechler, Terry
Fuchs, Elaine
author_facet Lechler, Terry
Fuchs, Elaine
author_sort Lechler, Terry
collection PubMed
description Despite their importance in cell shape and polarity generation, the organization of microtubules in differentiated cells and tissues remains relatively unexplored in mammals. We generated transgenic mice in which the epidermis expresses a fluorescently labeled microtubule-binding protein and show that in epidermis and in cultured keratinocytes, microtubules stereotypically reorganize as they differentiate. In basal cells, microtubules form a cytoplasmic network emanating from an apical centrosome. In suprabasal cells, microtubules concentrate at cell–cell junctions. The centrosome retains its ability to nucleate microtubules in differentiated cells, but no longer anchors them. During epidermal differentiation, ninein, which is a centrosomal protein required for microtubule anchoring (Dammermann, A., and A. Merdes. 2002. J. Cell Biol. 159:255–266; Delgehyr, N., J. Sillibourne, and M. Bornens. 2005. J. Cell Sci. 118:1565–1575; Mogensen, M.M., A. Malik, M. Piel, V. Bouckson-Castaing, and M. Bornens. 2000. J. Cell Sci. 113:3013–3023), is lost from the centrosome and is recruited to desmosomes by desmoplakin (DP). Loss of DP prevents accumulation of cortical microtubules in vivo and in vitro. Our work uncovers a differentiation-specific rearrangement of the microtubule cytoskeleton in epidermis, and defines an essential role for DP in the process.
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spelling pubmed-20639342007-11-29 Desmoplakin: an unexpected regulator of microtubule organization in the epidermis Lechler, Terry Fuchs, Elaine J Cell Biol Research Articles Despite their importance in cell shape and polarity generation, the organization of microtubules in differentiated cells and tissues remains relatively unexplored in mammals. We generated transgenic mice in which the epidermis expresses a fluorescently labeled microtubule-binding protein and show that in epidermis and in cultured keratinocytes, microtubules stereotypically reorganize as they differentiate. In basal cells, microtubules form a cytoplasmic network emanating from an apical centrosome. In suprabasal cells, microtubules concentrate at cell–cell junctions. The centrosome retains its ability to nucleate microtubules in differentiated cells, but no longer anchors them. During epidermal differentiation, ninein, which is a centrosomal protein required for microtubule anchoring (Dammermann, A., and A. Merdes. 2002. J. Cell Biol. 159:255–266; Delgehyr, N., J. Sillibourne, and M. Bornens. 2005. J. Cell Sci. 118:1565–1575; Mogensen, M.M., A. Malik, M. Piel, V. Bouckson-Castaing, and M. Bornens. 2000. J. Cell Sci. 113:3013–3023), is lost from the centrosome and is recruited to desmosomes by desmoplakin (DP). Loss of DP prevents accumulation of cortical microtubules in vivo and in vitro. Our work uncovers a differentiation-specific rearrangement of the microtubule cytoskeleton in epidermis, and defines an essential role for DP in the process. The Rockefeller University Press 2007-01-15 /pmc/articles/PMC2063934/ /pubmed/17227889 http://dx.doi.org/10.1083/jcb.200609109 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Lechler, Terry
Fuchs, Elaine
Desmoplakin: an unexpected regulator of microtubule organization in the epidermis
title Desmoplakin: an unexpected regulator of microtubule organization in the epidermis
title_full Desmoplakin: an unexpected regulator of microtubule organization in the epidermis
title_fullStr Desmoplakin: an unexpected regulator of microtubule organization in the epidermis
title_full_unstemmed Desmoplakin: an unexpected regulator of microtubule organization in the epidermis
title_short Desmoplakin: an unexpected regulator of microtubule organization in the epidermis
title_sort desmoplakin: an unexpected regulator of microtubule organization in the epidermis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063934/
https://www.ncbi.nlm.nih.gov/pubmed/17227889
http://dx.doi.org/10.1083/jcb.200609109
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