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Cardiac side population cells have a potential to migrate and differentiate into cardiomyocytes in vitro and in vivo

Side population (SP) cells, which can be identified by their ability to exclude Hoechst 33342 dye, are one of the candidates for somatic stem cells. Although bone marrow SP cells are known to be long-term repopulating hematopoietic stem cells, there is little information about the characteristics of...

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Autores principales: Oyama, Tomomi, Nagai, Toshio, Wada, Hiroshi, Naito, Atsuhiko Thomas, Matsuura, Katsuhisa, Iwanaga, Koji, Takahashi, Toshinao, Goto, Motohiro, Mikami, Yoko, Yasuda, Noritaka, Akazawa, Hiroshi, Uezumi, Akiyoshi, Takeda, Shin'ichi, Komuro, Issei
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063959/
https://www.ncbi.nlm.nih.gov/pubmed/17261849
http://dx.doi.org/10.1083/jcb.200603014
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author Oyama, Tomomi
Nagai, Toshio
Wada, Hiroshi
Naito, Atsuhiko Thomas
Matsuura, Katsuhisa
Iwanaga, Koji
Takahashi, Toshinao
Goto, Motohiro
Mikami, Yoko
Yasuda, Noritaka
Akazawa, Hiroshi
Uezumi, Akiyoshi
Takeda, Shin'ichi
Komuro, Issei
author_facet Oyama, Tomomi
Nagai, Toshio
Wada, Hiroshi
Naito, Atsuhiko Thomas
Matsuura, Katsuhisa
Iwanaga, Koji
Takahashi, Toshinao
Goto, Motohiro
Mikami, Yoko
Yasuda, Noritaka
Akazawa, Hiroshi
Uezumi, Akiyoshi
Takeda, Shin'ichi
Komuro, Issei
author_sort Oyama, Tomomi
collection PubMed
description Side population (SP) cells, which can be identified by their ability to exclude Hoechst 33342 dye, are one of the candidates for somatic stem cells. Although bone marrow SP cells are known to be long-term repopulating hematopoietic stem cells, there is little information about the characteristics of cardiac SP cells (CSPs). When cultured CSPs from neonatal rat hearts were treated with oxytocin or trichostatin A, some CSPs expressed cardiac-specific genes and proteins and showed spontaneous beating. When green fluorescent protein–positive CSPs were intravenously infused into adult rats, many more (∼12-fold) CSPs were migrated and homed in injured heart than in normal heart. CSPs in injured heart differentiated into cardiomyocytes, endothelial cells, or smooth muscle cells (4.4%, 6.7%, and 29% of total CSP-derived cells, respectively). These results suggest that CSPs are intrinsic cardiac stem cells and involved in the regeneration of diseased hearts.
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spelling pubmed-20639592007-11-29 Cardiac side population cells have a potential to migrate and differentiate into cardiomyocytes in vitro and in vivo Oyama, Tomomi Nagai, Toshio Wada, Hiroshi Naito, Atsuhiko Thomas Matsuura, Katsuhisa Iwanaga, Koji Takahashi, Toshinao Goto, Motohiro Mikami, Yoko Yasuda, Noritaka Akazawa, Hiroshi Uezumi, Akiyoshi Takeda, Shin'ichi Komuro, Issei J Cell Biol Research Articles Side population (SP) cells, which can be identified by their ability to exclude Hoechst 33342 dye, are one of the candidates for somatic stem cells. Although bone marrow SP cells are known to be long-term repopulating hematopoietic stem cells, there is little information about the characteristics of cardiac SP cells (CSPs). When cultured CSPs from neonatal rat hearts were treated with oxytocin or trichostatin A, some CSPs expressed cardiac-specific genes and proteins and showed spontaneous beating. When green fluorescent protein–positive CSPs were intravenously infused into adult rats, many more (∼12-fold) CSPs were migrated and homed in injured heart than in normal heart. CSPs in injured heart differentiated into cardiomyocytes, endothelial cells, or smooth muscle cells (4.4%, 6.7%, and 29% of total CSP-derived cells, respectively). These results suggest that CSPs are intrinsic cardiac stem cells and involved in the regeneration of diseased hearts. The Rockefeller University Press 2007-01-29 /pmc/articles/PMC2063959/ /pubmed/17261849 http://dx.doi.org/10.1083/jcb.200603014 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Oyama, Tomomi
Nagai, Toshio
Wada, Hiroshi
Naito, Atsuhiko Thomas
Matsuura, Katsuhisa
Iwanaga, Koji
Takahashi, Toshinao
Goto, Motohiro
Mikami, Yoko
Yasuda, Noritaka
Akazawa, Hiroshi
Uezumi, Akiyoshi
Takeda, Shin'ichi
Komuro, Issei
Cardiac side population cells have a potential to migrate and differentiate into cardiomyocytes in vitro and in vivo
title Cardiac side population cells have a potential to migrate and differentiate into cardiomyocytes in vitro and in vivo
title_full Cardiac side population cells have a potential to migrate and differentiate into cardiomyocytes in vitro and in vivo
title_fullStr Cardiac side population cells have a potential to migrate and differentiate into cardiomyocytes in vitro and in vivo
title_full_unstemmed Cardiac side population cells have a potential to migrate and differentiate into cardiomyocytes in vitro and in vivo
title_short Cardiac side population cells have a potential to migrate and differentiate into cardiomyocytes in vitro and in vivo
title_sort cardiac side population cells have a potential to migrate and differentiate into cardiomyocytes in vitro and in vivo
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2063959/
https://www.ncbi.nlm.nih.gov/pubmed/17261849
http://dx.doi.org/10.1083/jcb.200603014
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