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Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth

In budding yeast, two classes of post-Golgi secretory vesicles carrying different sets of cargoes typified by Bgl2p and invertase are delivered to the plasma membrane for secretion. The exocyst is implicated in tethering these vesicles to the daughter cell membrane for exocytosis. In this study, we...

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Detalles Bibliográficos
Autores principales: He, Bing, Xi, Fengong, Zhang, Jian, TerBush, Daniel, Zhang, Xiaoyu, Guo, Wei
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064051/
https://www.ncbi.nlm.nih.gov/pubmed/17339375
http://dx.doi.org/10.1083/jcb.200606134
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author He, Bing
Xi, Fengong
Zhang, Jian
TerBush, Daniel
Zhang, Xiaoyu
Guo, Wei
author_facet He, Bing
Xi, Fengong
Zhang, Jian
TerBush, Daniel
Zhang, Xiaoyu
Guo, Wei
author_sort He, Bing
collection PubMed
description In budding yeast, two classes of post-Golgi secretory vesicles carrying different sets of cargoes typified by Bgl2p and invertase are delivered to the plasma membrane for secretion. The exocyst is implicated in tethering these vesicles to the daughter cell membrane for exocytosis. In this study, we report that mutations in the exocyst component Exo70p predominantly block secretion of the Bgl2p vesicles. Furthermore, a defect in invertase vesicle trafficking caused by vps1Δ or pep12Δ in the exo70 mutant background is detrimental to the cell. The secretion defect in exo70 mutants was most pronounced during the early budding stage, which affected daughter cell growth. The selective secretion block does not occur at the vesicle formation or sorting stage because the exocytic vesicles are properly generated and protein processing is normal in the exo70 mutants. Our study suggests that Exo70p functions primarily at early stages of the cell cycle in Bgl2p vesicle secretion, which is critical for polarized cell growth.
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spelling pubmed-20640512007-11-29 Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth He, Bing Xi, Fengong Zhang, Jian TerBush, Daniel Zhang, Xiaoyu Guo, Wei J Cell Biol Research Articles In budding yeast, two classes of post-Golgi secretory vesicles carrying different sets of cargoes typified by Bgl2p and invertase are delivered to the plasma membrane for secretion. The exocyst is implicated in tethering these vesicles to the daughter cell membrane for exocytosis. In this study, we report that mutations in the exocyst component Exo70p predominantly block secretion of the Bgl2p vesicles. Furthermore, a defect in invertase vesicle trafficking caused by vps1Δ or pep12Δ in the exo70 mutant background is detrimental to the cell. The secretion defect in exo70 mutants was most pronounced during the early budding stage, which affected daughter cell growth. The selective secretion block does not occur at the vesicle formation or sorting stage because the exocytic vesicles are properly generated and protein processing is normal in the exo70 mutants. Our study suggests that Exo70p functions primarily at early stages of the cell cycle in Bgl2p vesicle secretion, which is critical for polarized cell growth. The Rockefeller University Press 2007-03-12 /pmc/articles/PMC2064051/ /pubmed/17339375 http://dx.doi.org/10.1083/jcb.200606134 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
He, Bing
Xi, Fengong
Zhang, Jian
TerBush, Daniel
Zhang, Xiaoyu
Guo, Wei
Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth
title Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth
title_full Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth
title_fullStr Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth
title_full_unstemmed Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth
title_short Exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth
title_sort exo70p mediates the secretion of specific exocytic vesicles at early stages of the cell cycle for polarized cell growth
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064051/
https://www.ncbi.nlm.nih.gov/pubmed/17339375
http://dx.doi.org/10.1083/jcb.200606134
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