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A role for cell sex in stem cell–mediated skeletal muscle regeneration: female cells have higher muscle regeneration efficiency
We have shown that muscle-derived stem cells (MDSCs) transplanted into dystrophic (mdx) mice efficiently regenerate skeletal muscle. However, MDSC populations exhibit heterogeneity in marker profiles and variability in regeneration abilities. We show here that cell sex is a variable that considerabl...
Autores principales: | , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064113/ https://www.ncbi.nlm.nih.gov/pubmed/17420291 http://dx.doi.org/10.1083/jcb.200612094 |
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author | Deasy, Bridget M. Lu, Aiping Tebbets, Jessica C. Feduska, Joseph M. Schugar, Rebecca C. Pollett, Jonathan B. Sun, Bin Urish, Kenneth L. Gharaibeh, Burhan M. Cao, Baohong Rubin, Robert T. Huard, Johnny |
author_facet | Deasy, Bridget M. Lu, Aiping Tebbets, Jessica C. Feduska, Joseph M. Schugar, Rebecca C. Pollett, Jonathan B. Sun, Bin Urish, Kenneth L. Gharaibeh, Burhan M. Cao, Baohong Rubin, Robert T. Huard, Johnny |
author_sort | Deasy, Bridget M. |
collection | PubMed |
description | We have shown that muscle-derived stem cells (MDSCs) transplanted into dystrophic (mdx) mice efficiently regenerate skeletal muscle. However, MDSC populations exhibit heterogeneity in marker profiles and variability in regeneration abilities. We show here that cell sex is a variable that considerably influences MDSCs' regeneration abilities. We found that the female MDSCs (F-MDSCs) regenerated skeletal muscle more efficiently. Despite using additional isolation techniques and cell cloning, we could not obtain a male subfraction with a regeneration capacity similar to that of their female counterparts. Rather than being directly hormonal or caused by host immune response, this difference in MDSCs' regeneration potential may arise from innate sex-related differences in the cells' stress responses. In comparison with F-MDSCs, male MDSCs have increased differentiation after exposure to oxidative stress induced by hydrogen peroxide, which may lead to in vivo donor cell depletion, and a proliferative advantage for F-MDSCs that eventually increases muscle regeneration. These findings should persuade researchers to report cell sex, which is a largely unexplored variable, and consider the implications of relying on cells of one sex. |
format | Text |
id | pubmed-2064113 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20641132007-11-29 A role for cell sex in stem cell–mediated skeletal muscle regeneration: female cells have higher muscle regeneration efficiency Deasy, Bridget M. Lu, Aiping Tebbets, Jessica C. Feduska, Joseph M. Schugar, Rebecca C. Pollett, Jonathan B. Sun, Bin Urish, Kenneth L. Gharaibeh, Burhan M. Cao, Baohong Rubin, Robert T. Huard, Johnny J Cell Biol Research Articles We have shown that muscle-derived stem cells (MDSCs) transplanted into dystrophic (mdx) mice efficiently regenerate skeletal muscle. However, MDSC populations exhibit heterogeneity in marker profiles and variability in regeneration abilities. We show here that cell sex is a variable that considerably influences MDSCs' regeneration abilities. We found that the female MDSCs (F-MDSCs) regenerated skeletal muscle more efficiently. Despite using additional isolation techniques and cell cloning, we could not obtain a male subfraction with a regeneration capacity similar to that of their female counterparts. Rather than being directly hormonal or caused by host immune response, this difference in MDSCs' regeneration potential may arise from innate sex-related differences in the cells' stress responses. In comparison with F-MDSCs, male MDSCs have increased differentiation after exposure to oxidative stress induced by hydrogen peroxide, which may lead to in vivo donor cell depletion, and a proliferative advantage for F-MDSCs that eventually increases muscle regeneration. These findings should persuade researchers to report cell sex, which is a largely unexplored variable, and consider the implications of relying on cells of one sex. The Rockefeller University Press 2007-04-09 /pmc/articles/PMC2064113/ /pubmed/17420291 http://dx.doi.org/10.1083/jcb.200612094 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Deasy, Bridget M. Lu, Aiping Tebbets, Jessica C. Feduska, Joseph M. Schugar, Rebecca C. Pollett, Jonathan B. Sun, Bin Urish, Kenneth L. Gharaibeh, Burhan M. Cao, Baohong Rubin, Robert T. Huard, Johnny A role for cell sex in stem cell–mediated skeletal muscle regeneration: female cells have higher muscle regeneration efficiency |
title | A role for cell sex in stem cell–mediated skeletal muscle regeneration: female cells have higher muscle regeneration efficiency |
title_full | A role for cell sex in stem cell–mediated skeletal muscle regeneration: female cells have higher muscle regeneration efficiency |
title_fullStr | A role for cell sex in stem cell–mediated skeletal muscle regeneration: female cells have higher muscle regeneration efficiency |
title_full_unstemmed | A role for cell sex in stem cell–mediated skeletal muscle regeneration: female cells have higher muscle regeneration efficiency |
title_short | A role for cell sex in stem cell–mediated skeletal muscle regeneration: female cells have higher muscle regeneration efficiency |
title_sort | role for cell sex in stem cell–mediated skeletal muscle regeneration: female cells have higher muscle regeneration efficiency |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064113/ https://www.ncbi.nlm.nih.gov/pubmed/17420291 http://dx.doi.org/10.1083/jcb.200612094 |
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