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Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling
Amajor function of the endocytic system is the sorting of cargo to various organelles. Endocytic sorting of the yeast reductive iron transporter, which is composed of the Fet3 and Ftr1 proteins, is regulated by available iron. When iron is provided to iron-starved cells, Fet3p–Ftr1p is targeted to t...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064116/ https://www.ncbi.nlm.nih.gov/pubmed/17420293 http://dx.doi.org/10.1083/jcb.200609161 |
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author | Strochlic, Todd I. Setty, Thanuja Gangi Sitaram, Anand Burd, Christopher G. |
author_facet | Strochlic, Todd I. Setty, Thanuja Gangi Sitaram, Anand Burd, Christopher G. |
author_sort | Strochlic, Todd I. |
collection | PubMed |
description | Amajor function of the endocytic system is the sorting of cargo to various organelles. Endocytic sorting of the yeast reductive iron transporter, which is composed of the Fet3 and Ftr1 proteins, is regulated by available iron. When iron is provided to iron-starved cells, Fet3p–Ftr1p is targeted to the lysosome-like vacuole and degraded. In contrast, when iron is not available, Fet3p–Ftr1p is maintained on the plasma membrane via an endocytic recycling pathway requiring the sorting nexin Grd19/Snx3p, the pentameric retromer complex, and the Ypt6p Golgi Rab GTPase module. A recycling signal in Ftr1p was identified and found to bind directly to Grd19/Snx3p. Retromer and Grd19/Snx3p partially colocalize to tubular endosomes, where they are physically associated. After export from the endosome, Fet3p–Ftr1p transits through the Golgi apparatus for resecretion. Thus, Grd19/Snx3p, functions as a cargo-specific adapter for the retromer complex, establishing a precedent for a mechanism by which sorting nexins expand the repertoire of retromer-dependent cargos. |
format | Text |
id | pubmed-2064116 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20641162007-11-29 Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling Strochlic, Todd I. Setty, Thanuja Gangi Sitaram, Anand Burd, Christopher G. J Cell Biol Research Articles Amajor function of the endocytic system is the sorting of cargo to various organelles. Endocytic sorting of the yeast reductive iron transporter, which is composed of the Fet3 and Ftr1 proteins, is regulated by available iron. When iron is provided to iron-starved cells, Fet3p–Ftr1p is targeted to the lysosome-like vacuole and degraded. In contrast, when iron is not available, Fet3p–Ftr1p is maintained on the plasma membrane via an endocytic recycling pathway requiring the sorting nexin Grd19/Snx3p, the pentameric retromer complex, and the Ypt6p Golgi Rab GTPase module. A recycling signal in Ftr1p was identified and found to bind directly to Grd19/Snx3p. Retromer and Grd19/Snx3p partially colocalize to tubular endosomes, where they are physically associated. After export from the endosome, Fet3p–Ftr1p transits through the Golgi apparatus for resecretion. Thus, Grd19/Snx3p, functions as a cargo-specific adapter for the retromer complex, establishing a precedent for a mechanism by which sorting nexins expand the repertoire of retromer-dependent cargos. The Rockefeller University Press 2007-04-09 /pmc/articles/PMC2064116/ /pubmed/17420293 http://dx.doi.org/10.1083/jcb.200609161 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Strochlic, Todd I. Setty, Thanuja Gangi Sitaram, Anand Burd, Christopher G. Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling |
title | Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling |
title_full | Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling |
title_fullStr | Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling |
title_full_unstemmed | Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling |
title_short | Grd19/Snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling |
title_sort | grd19/snx3p functions as a cargo-specific adapter for retromer-dependent endocytic recycling |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064116/ https://www.ncbi.nlm.nih.gov/pubmed/17420293 http://dx.doi.org/10.1083/jcb.200609161 |
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