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Myosin-1a powers the sliding of apical membrane along microvillar actin bundles
Microvilli are actin-rich membrane protrusions common to a variety of epithelial cell types. Within microvilli of the enterocyte brush border (BB), myosin-1a (Myo1a) forms an ordered ensemble of bridges that link the plasma membrane to the underlying polarized actin bundle. Despite decades of invest...
Autores principales: | , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064212/ https://www.ncbi.nlm.nih.gov/pubmed/17502425 http://dx.doi.org/10.1083/jcb.200701144 |
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author | McConnell, Russell E. Tyska, Matthew J. |
author_facet | McConnell, Russell E. Tyska, Matthew J. |
author_sort | McConnell, Russell E. |
collection | PubMed |
description | Microvilli are actin-rich membrane protrusions common to a variety of epithelial cell types. Within microvilli of the enterocyte brush border (BB), myosin-1a (Myo1a) forms an ordered ensemble of bridges that link the plasma membrane to the underlying polarized actin bundle. Despite decades of investigation, the function of this unique actomyosin array has remained unclear. Here, we show that addition of ATP to isolated BBs induces a plus end–directed translation of apical membrane along microvillar actin bundles. Upon reaching microvillar tips, membrane is “shed” into solution in the form of small vesicles. Because this movement demonstrates the polarity, velocity, and nucleotide dependence expected for a Myo1a-driven process, and BBs lacking Myo1a fail to undergo membrane translation, we conclude that Myo1a powers this novel form of motility. Thus, in addition to providing a means for amplifying apical surface area, we propose that microvilli function as actomyosin contractile arrays that power the release of BB membrane vesicles into the intestinal lumen. |
format | Text |
id | pubmed-2064212 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20642122007-11-29 Myosin-1a powers the sliding of apical membrane along microvillar actin bundles McConnell, Russell E. Tyska, Matthew J. J Cell Biol Research Articles Microvilli are actin-rich membrane protrusions common to a variety of epithelial cell types. Within microvilli of the enterocyte brush border (BB), myosin-1a (Myo1a) forms an ordered ensemble of bridges that link the plasma membrane to the underlying polarized actin bundle. Despite decades of investigation, the function of this unique actomyosin array has remained unclear. Here, we show that addition of ATP to isolated BBs induces a plus end–directed translation of apical membrane along microvillar actin bundles. Upon reaching microvillar tips, membrane is “shed” into solution in the form of small vesicles. Because this movement demonstrates the polarity, velocity, and nucleotide dependence expected for a Myo1a-driven process, and BBs lacking Myo1a fail to undergo membrane translation, we conclude that Myo1a powers this novel form of motility. Thus, in addition to providing a means for amplifying apical surface area, we propose that microvilli function as actomyosin contractile arrays that power the release of BB membrane vesicles into the intestinal lumen. The Rockefeller University Press 2007-05-21 /pmc/articles/PMC2064212/ /pubmed/17502425 http://dx.doi.org/10.1083/jcb.200701144 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles McConnell, Russell E. Tyska, Matthew J. Myosin-1a powers the sliding of apical membrane along microvillar actin bundles |
title | Myosin-1a powers the sliding of apical membrane along microvillar actin bundles |
title_full | Myosin-1a powers the sliding of apical membrane along microvillar actin bundles |
title_fullStr | Myosin-1a powers the sliding of apical membrane along microvillar actin bundles |
title_full_unstemmed | Myosin-1a powers the sliding of apical membrane along microvillar actin bundles |
title_short | Myosin-1a powers the sliding of apical membrane along microvillar actin bundles |
title_sort | myosin-1a powers the sliding of apical membrane along microvillar actin bundles |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064212/ https://www.ncbi.nlm.nih.gov/pubmed/17502425 http://dx.doi.org/10.1083/jcb.200701144 |
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