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Myosin-1a powers the sliding of apical membrane along microvillar actin bundles

Microvilli are actin-rich membrane protrusions common to a variety of epithelial cell types. Within microvilli of the enterocyte brush border (BB), myosin-1a (Myo1a) forms an ordered ensemble of bridges that link the plasma membrane to the underlying polarized actin bundle. Despite decades of invest...

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Detalles Bibliográficos
Autores principales: McConnell, Russell E., Tyska, Matthew J.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064212/
https://www.ncbi.nlm.nih.gov/pubmed/17502425
http://dx.doi.org/10.1083/jcb.200701144
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author McConnell, Russell E.
Tyska, Matthew J.
author_facet McConnell, Russell E.
Tyska, Matthew J.
author_sort McConnell, Russell E.
collection PubMed
description Microvilli are actin-rich membrane protrusions common to a variety of epithelial cell types. Within microvilli of the enterocyte brush border (BB), myosin-1a (Myo1a) forms an ordered ensemble of bridges that link the plasma membrane to the underlying polarized actin bundle. Despite decades of investigation, the function of this unique actomyosin array has remained unclear. Here, we show that addition of ATP to isolated BBs induces a plus end–directed translation of apical membrane along microvillar actin bundles. Upon reaching microvillar tips, membrane is “shed” into solution in the form of small vesicles. Because this movement demonstrates the polarity, velocity, and nucleotide dependence expected for a Myo1a-driven process, and BBs lacking Myo1a fail to undergo membrane translation, we conclude that Myo1a powers this novel form of motility. Thus, in addition to providing a means for amplifying apical surface area, we propose that microvilli function as actomyosin contractile arrays that power the release of BB membrane vesicles into the intestinal lumen.
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spelling pubmed-20642122007-11-29 Myosin-1a powers the sliding of apical membrane along microvillar actin bundles McConnell, Russell E. Tyska, Matthew J. J Cell Biol Research Articles Microvilli are actin-rich membrane protrusions common to a variety of epithelial cell types. Within microvilli of the enterocyte brush border (BB), myosin-1a (Myo1a) forms an ordered ensemble of bridges that link the plasma membrane to the underlying polarized actin bundle. Despite decades of investigation, the function of this unique actomyosin array has remained unclear. Here, we show that addition of ATP to isolated BBs induces a plus end–directed translation of apical membrane along microvillar actin bundles. Upon reaching microvillar tips, membrane is “shed” into solution in the form of small vesicles. Because this movement demonstrates the polarity, velocity, and nucleotide dependence expected for a Myo1a-driven process, and BBs lacking Myo1a fail to undergo membrane translation, we conclude that Myo1a powers this novel form of motility. Thus, in addition to providing a means for amplifying apical surface area, we propose that microvilli function as actomyosin contractile arrays that power the release of BB membrane vesicles into the intestinal lumen. The Rockefeller University Press 2007-05-21 /pmc/articles/PMC2064212/ /pubmed/17502425 http://dx.doi.org/10.1083/jcb.200701144 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
McConnell, Russell E.
Tyska, Matthew J.
Myosin-1a powers the sliding of apical membrane along microvillar actin bundles
title Myosin-1a powers the sliding of apical membrane along microvillar actin bundles
title_full Myosin-1a powers the sliding of apical membrane along microvillar actin bundles
title_fullStr Myosin-1a powers the sliding of apical membrane along microvillar actin bundles
title_full_unstemmed Myosin-1a powers the sliding of apical membrane along microvillar actin bundles
title_short Myosin-1a powers the sliding of apical membrane along microvillar actin bundles
title_sort myosin-1a powers the sliding of apical membrane along microvillar actin bundles
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064212/
https://www.ncbi.nlm.nih.gov/pubmed/17502425
http://dx.doi.org/10.1083/jcb.200701144
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