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Requirement of dendritic Akt degradation by the ubiquitin–proteasome system for neuronal polarity
Asymmetric distributions of activities of the protein kinases Akt and glycogen synthase kinase 3β (GSK-3β) are critical for the formation of neuronal polarity. However, the mechanisms underlying polarized regulation of this pathway remain unclear. In this study, we report that the instability of Akt...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064237/ https://www.ncbi.nlm.nih.gov/pubmed/16864652 http://dx.doi.org/10.1083/jcb.200511028 |
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author | Yan, Dong Guo, Li Wang, Yizheng |
author_facet | Yan, Dong Guo, Li Wang, Yizheng |
author_sort | Yan, Dong |
collection | PubMed |
description | Asymmetric distributions of activities of the protein kinases Akt and glycogen synthase kinase 3β (GSK-3β) are critical for the formation of neuronal polarity. However, the mechanisms underlying polarized regulation of this pathway remain unclear. In this study, we report that the instability of Akt regulated by the ubiquitin–proteasome system (UPS) is required for neuron polarity. Preferential distribution in the axons was observed for Akt but not for its target GSK-3β. A photoactivatable GFP fused to Akt revealed the preferential instability of Akt in dendrites. Akt but not p110 or GSK-3β was ubiquitinated. Suppressing the UPS led to the symmetric distribution of Akt and the formation of multiple axons. These results indicate that local protein degradation mediated by the UPS is important in determining neuronal polarity. |
format | Text |
id | pubmed-2064237 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20642372007-11-29 Requirement of dendritic Akt degradation by the ubiquitin–proteasome system for neuronal polarity Yan, Dong Guo, Li Wang, Yizheng J Cell Biol Research Articles Asymmetric distributions of activities of the protein kinases Akt and glycogen synthase kinase 3β (GSK-3β) are critical for the formation of neuronal polarity. However, the mechanisms underlying polarized regulation of this pathway remain unclear. In this study, we report that the instability of Akt regulated by the ubiquitin–proteasome system (UPS) is required for neuron polarity. Preferential distribution in the axons was observed for Akt but not for its target GSK-3β. A photoactivatable GFP fused to Akt revealed the preferential instability of Akt in dendrites. Akt but not p110 or GSK-3β was ubiquitinated. Suppressing the UPS led to the symmetric distribution of Akt and the formation of multiple axons. These results indicate that local protein degradation mediated by the UPS is important in determining neuronal polarity. The Rockefeller University Press 2006-07-31 /pmc/articles/PMC2064237/ /pubmed/16864652 http://dx.doi.org/10.1083/jcb.200511028 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Yan, Dong Guo, Li Wang, Yizheng Requirement of dendritic Akt degradation by the ubiquitin–proteasome system for neuronal polarity |
title | Requirement of dendritic Akt degradation by the ubiquitin–proteasome system for neuronal polarity |
title_full | Requirement of dendritic Akt degradation by the ubiquitin–proteasome system for neuronal polarity |
title_fullStr | Requirement of dendritic Akt degradation by the ubiquitin–proteasome system for neuronal polarity |
title_full_unstemmed | Requirement of dendritic Akt degradation by the ubiquitin–proteasome system for neuronal polarity |
title_short | Requirement of dendritic Akt degradation by the ubiquitin–proteasome system for neuronal polarity |
title_sort | requirement of dendritic akt degradation by the ubiquitin–proteasome system for neuronal polarity |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064237/ https://www.ncbi.nlm.nih.gov/pubmed/16864652 http://dx.doi.org/10.1083/jcb.200511028 |
work_keys_str_mv | AT yandong requirementofdendriticaktdegradationbytheubiquitinproteasomesystemforneuronalpolarity AT guoli requirementofdendriticaktdegradationbytheubiquitinproteasomesystemforneuronalpolarity AT wangyizheng requirementofdendriticaktdegradationbytheubiquitinproteasomesystemforneuronalpolarity |