Cargando…

Muscle-specific microRNA miR-206 promotes muscle differentiation

Three muscle-specific microRNAs, miR-206, -1, and -133, are induced during differentiation of C2C12 myoblasts in vitro. Transfection of miR-206 promotes differentiation despite the presence of serum, whereas inhibition of the microRNA by antisense oligonucleotide inhibits cell cycle withdrawal and d...

Descripción completa

Detalles Bibliográficos
Autores principales: Kim, Hak Kyun, Lee, Yong Sun, Sivaprasad, Umasundari, Malhotra, Ankit, Dutta, Anindya
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064311/
https://www.ncbi.nlm.nih.gov/pubmed/16923828
http://dx.doi.org/10.1083/jcb.200603008
_version_ 1782137508940742656
author Kim, Hak Kyun
Lee, Yong Sun
Sivaprasad, Umasundari
Malhotra, Ankit
Dutta, Anindya
author_facet Kim, Hak Kyun
Lee, Yong Sun
Sivaprasad, Umasundari
Malhotra, Ankit
Dutta, Anindya
author_sort Kim, Hak Kyun
collection PubMed
description Three muscle-specific microRNAs, miR-206, -1, and -133, are induced during differentiation of C2C12 myoblasts in vitro. Transfection of miR-206 promotes differentiation despite the presence of serum, whereas inhibition of the microRNA by antisense oligonucleotide inhibits cell cycle withdrawal and differentiation, which are normally induced by serum deprivation. Among the many mRNAs that are down-regulated by miR-206, the p180 subunit of DNA polymerase α and three other genes are shown to be direct targets. Down-regulation of the polymerase inhibits DNA synthesis, an important component of the differentiation program. The direct targets are decreased by mRNA cleavage that is dependent on predicted microRNA target sites. Unlike small interfering RNA–directed cleavage, however, the 5′ ends of the cleavage fragments are distributed and not confined to the target sites, suggesting involvement of exonucleases in the degradation process. In addition, inhibitors of myogenic transcription factors, Id1-3 and MyoR, are decreased upon miR-206 introduction, suggesting the presence of additional mechanisms by which microRNAs enforce the differentiation program.
format Text
id pubmed-2064311
institution National Center for Biotechnology Information
language English
publishDate 2006
publisher The Rockefeller University Press
record_format MEDLINE/PubMed
spelling pubmed-20643112007-11-29 Muscle-specific microRNA miR-206 promotes muscle differentiation Kim, Hak Kyun Lee, Yong Sun Sivaprasad, Umasundari Malhotra, Ankit Dutta, Anindya J Cell Biol Research Articles Three muscle-specific microRNAs, miR-206, -1, and -133, are induced during differentiation of C2C12 myoblasts in vitro. Transfection of miR-206 promotes differentiation despite the presence of serum, whereas inhibition of the microRNA by antisense oligonucleotide inhibits cell cycle withdrawal and differentiation, which are normally induced by serum deprivation. Among the many mRNAs that are down-regulated by miR-206, the p180 subunit of DNA polymerase α and three other genes are shown to be direct targets. Down-regulation of the polymerase inhibits DNA synthesis, an important component of the differentiation program. The direct targets are decreased by mRNA cleavage that is dependent on predicted microRNA target sites. Unlike small interfering RNA–directed cleavage, however, the 5′ ends of the cleavage fragments are distributed and not confined to the target sites, suggesting involvement of exonucleases in the degradation process. In addition, inhibitors of myogenic transcription factors, Id1-3 and MyoR, are decreased upon miR-206 introduction, suggesting the presence of additional mechanisms by which microRNAs enforce the differentiation program. The Rockefeller University Press 2006-08-28 /pmc/articles/PMC2064311/ /pubmed/16923828 http://dx.doi.org/10.1083/jcb.200603008 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Kim, Hak Kyun
Lee, Yong Sun
Sivaprasad, Umasundari
Malhotra, Ankit
Dutta, Anindya
Muscle-specific microRNA miR-206 promotes muscle differentiation
title Muscle-specific microRNA miR-206 promotes muscle differentiation
title_full Muscle-specific microRNA miR-206 promotes muscle differentiation
title_fullStr Muscle-specific microRNA miR-206 promotes muscle differentiation
title_full_unstemmed Muscle-specific microRNA miR-206 promotes muscle differentiation
title_short Muscle-specific microRNA miR-206 promotes muscle differentiation
title_sort muscle-specific microrna mir-206 promotes muscle differentiation
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064311/
https://www.ncbi.nlm.nih.gov/pubmed/16923828
http://dx.doi.org/10.1083/jcb.200603008
work_keys_str_mv AT kimhakkyun musclespecificmicrornamir206promotesmuscledifferentiation
AT leeyongsun musclespecificmicrornamir206promotesmuscledifferentiation
AT sivaprasadumasundari musclespecificmicrornamir206promotesmuscledifferentiation
AT malhotraankit musclespecificmicrornamir206promotesmuscledifferentiation
AT duttaanindya musclespecificmicrornamir206promotesmuscledifferentiation