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Autoinhibition regulates cellular localization and actin assembly activity of the diaphanous-related formins FRLα and mDia1
Diaphanous-related formins (DRFs) are key regulators of actin cytoskeletal dynamics whose in vitro actin assembly activities are thought to be regulated by autoinhibition. However, the in vivo consequences of autoinhibition and the involvement of DRFs in specific biological processes are not well un...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2006
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064313/ https://www.ncbi.nlm.nih.gov/pubmed/16943183 http://dx.doi.org/10.1083/jcb.200605006 |
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author | Seth, Abhinav Otomo, Chinatsu Rosen, Michael K. |
author_facet | Seth, Abhinav Otomo, Chinatsu Rosen, Michael K. |
author_sort | Seth, Abhinav |
collection | PubMed |
description | Diaphanous-related formins (DRFs) are key regulators of actin cytoskeletal dynamics whose in vitro actin assembly activities are thought to be regulated by autoinhibition. However, the in vivo consequences of autoinhibition and the involvement of DRFs in specific biological processes are not well understood. In this study, we show that in the DRFs FRLα (formin-related gene in leukocytes α) and mouse diaphanous 1, autoinhibition regulates a novel membrane localization activity in vivo as well as actin assembly activity in vitro. In FRLα, the Rho family guanosine triphosphatase Cdc42 relieves the autoinhibition of both membrane localization and biochemical actin assembly activities. FRLα is required for efficient Fc-γ receptor–mediated phagocytosis and is recruited to the phagocytic cup by Cdc42. These results suggest that mutual autoinhibition of biochemical activity and cellular localization may be a general regulatory principle for DRFs and demonstrate a novel role for formins in immune function. |
format | Text |
id | pubmed-2064313 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2006 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20643132007-11-29 Autoinhibition regulates cellular localization and actin assembly activity of the diaphanous-related formins FRLα and mDia1 Seth, Abhinav Otomo, Chinatsu Rosen, Michael K. J Cell Biol Research Articles Diaphanous-related formins (DRFs) are key regulators of actin cytoskeletal dynamics whose in vitro actin assembly activities are thought to be regulated by autoinhibition. However, the in vivo consequences of autoinhibition and the involvement of DRFs in specific biological processes are not well understood. In this study, we show that in the DRFs FRLα (formin-related gene in leukocytes α) and mouse diaphanous 1, autoinhibition regulates a novel membrane localization activity in vivo as well as actin assembly activity in vitro. In FRLα, the Rho family guanosine triphosphatase Cdc42 relieves the autoinhibition of both membrane localization and biochemical actin assembly activities. FRLα is required for efficient Fc-γ receptor–mediated phagocytosis and is recruited to the phagocytic cup by Cdc42. These results suggest that mutual autoinhibition of biochemical activity and cellular localization may be a general regulatory principle for DRFs and demonstrate a novel role for formins in immune function. The Rockefeller University Press 2006-08-28 /pmc/articles/PMC2064313/ /pubmed/16943183 http://dx.doi.org/10.1083/jcb.200605006 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Seth, Abhinav Otomo, Chinatsu Rosen, Michael K. Autoinhibition regulates cellular localization and actin assembly activity of the diaphanous-related formins FRLα and mDia1 |
title | Autoinhibition regulates cellular localization and actin assembly activity of the diaphanous-related formins FRLα and mDia1 |
title_full | Autoinhibition regulates cellular localization and actin assembly activity of the diaphanous-related formins FRLα and mDia1 |
title_fullStr | Autoinhibition regulates cellular localization and actin assembly activity of the diaphanous-related formins FRLα and mDia1 |
title_full_unstemmed | Autoinhibition regulates cellular localization and actin assembly activity of the diaphanous-related formins FRLα and mDia1 |
title_short | Autoinhibition regulates cellular localization and actin assembly activity of the diaphanous-related formins FRLα and mDia1 |
title_sort | autoinhibition regulates cellular localization and actin assembly activity of the diaphanous-related formins frlα and mdia1 |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064313/ https://www.ncbi.nlm.nih.gov/pubmed/16943183 http://dx.doi.org/10.1083/jcb.200605006 |
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