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Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography

Three-dimensional images of the undercoat structure on the cytoplasmic surface of the upper cell membrane of normal rat kidney fibroblast (NRK) cells and fetal rat skin keratinocytes were reconstructed by electron tomography, with 0.85-nm–thick consecutive sections made ∼100 nm from the cytoplasmic...

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Autores principales: Morone, Nobuhiro, Fujiwara, Takahiro, Murase, Kotono, Kasai, Rinshi S., Ike, Hiroshi, Yuasa, Shigeki, Usukura, Jiro, Kusumi, Akihiro
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2006
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064339/
https://www.ncbi.nlm.nih.gov/pubmed/16954349
http://dx.doi.org/10.1083/jcb.200606007
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author Morone, Nobuhiro
Fujiwara, Takahiro
Murase, Kotono
Kasai, Rinshi S.
Ike, Hiroshi
Yuasa, Shigeki
Usukura, Jiro
Kusumi, Akihiro
author_facet Morone, Nobuhiro
Fujiwara, Takahiro
Murase, Kotono
Kasai, Rinshi S.
Ike, Hiroshi
Yuasa, Shigeki
Usukura, Jiro
Kusumi, Akihiro
author_sort Morone, Nobuhiro
collection PubMed
description Three-dimensional images of the undercoat structure on the cytoplasmic surface of the upper cell membrane of normal rat kidney fibroblast (NRK) cells and fetal rat skin keratinocytes were reconstructed by electron tomography, with 0.85-nm–thick consecutive sections made ∼100 nm from the cytoplasmic surface using rapidly frozen, deeply etched, platinum-replicated plasma membranes. The membrane skeleton (MSK) primarily consists of actin filaments and associated proteins. The MSK covers the entire cytoplasmic surface and is closely linked to clathrin-coated pits and caveolae. The actin filaments that are closely apposed to the cytoplasmic surface of the plasma membrane (within 10.2 nm) are likely to form the boundaries of the membrane compartments responsible for the temporary confinement of membrane molecules, thus partitioning the plasma membrane with regard to their lateral diffusion. The distribution of the MSK mesh size as determined by electron tomography and that of the compartment size as determined from high speed single-particle tracking of phospholipid diffusion agree well in both cell types, supporting the MSK fence and MSK-anchored protein picket models.
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spelling pubmed-20643392007-11-29 Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography Morone, Nobuhiro Fujiwara, Takahiro Murase, Kotono Kasai, Rinshi S. Ike, Hiroshi Yuasa, Shigeki Usukura, Jiro Kusumi, Akihiro J Cell Biol Research Articles Three-dimensional images of the undercoat structure on the cytoplasmic surface of the upper cell membrane of normal rat kidney fibroblast (NRK) cells and fetal rat skin keratinocytes were reconstructed by electron tomography, with 0.85-nm–thick consecutive sections made ∼100 nm from the cytoplasmic surface using rapidly frozen, deeply etched, platinum-replicated plasma membranes. The membrane skeleton (MSK) primarily consists of actin filaments and associated proteins. The MSK covers the entire cytoplasmic surface and is closely linked to clathrin-coated pits and caveolae. The actin filaments that are closely apposed to the cytoplasmic surface of the plasma membrane (within 10.2 nm) are likely to form the boundaries of the membrane compartments responsible for the temporary confinement of membrane molecules, thus partitioning the plasma membrane with regard to their lateral diffusion. The distribution of the MSK mesh size as determined by electron tomography and that of the compartment size as determined from high speed single-particle tracking of phospholipid diffusion agree well in both cell types, supporting the MSK fence and MSK-anchored protein picket models. The Rockefeller University Press 2006-09-11 /pmc/articles/PMC2064339/ /pubmed/16954349 http://dx.doi.org/10.1083/jcb.200606007 Text en Copyright © 2006, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Morone, Nobuhiro
Fujiwara, Takahiro
Murase, Kotono
Kasai, Rinshi S.
Ike, Hiroshi
Yuasa, Shigeki
Usukura, Jiro
Kusumi, Akihiro
Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography
title Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography
title_full Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography
title_fullStr Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography
title_full_unstemmed Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography
title_short Three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography
title_sort three-dimensional reconstruction of the membrane skeleton at the plasma membrane interface by electron tomography
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064339/
https://www.ncbi.nlm.nih.gov/pubmed/16954349
http://dx.doi.org/10.1083/jcb.200606007
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