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Organization of chromatin and histone modifications at a transcription site

According to the transcription factory model, localized transcription sites composed of immobilized polymerase molecules transcribe chromatin by reeling it through the transcription site and extruding it to form a surrounding domain of recently transcribed decondensed chromatin. Although transcripti...

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Detalles Bibliográficos
Autores principales: Müller, Waltraud G., Rieder, Dietmar, Karpova, Tatiana S., John, Sam, Trajanoski, Zlatko, McNally, James G.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064357/
https://www.ncbi.nlm.nih.gov/pubmed/17576795
http://dx.doi.org/10.1083/jcb.200703157
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author Müller, Waltraud G.
Rieder, Dietmar
Karpova, Tatiana S.
John, Sam
Trajanoski, Zlatko
McNally, James G.
author_facet Müller, Waltraud G.
Rieder, Dietmar
Karpova, Tatiana S.
John, Sam
Trajanoski, Zlatko
McNally, James G.
author_sort Müller, Waltraud G.
collection PubMed
description According to the transcription factory model, localized transcription sites composed of immobilized polymerase molecules transcribe chromatin by reeling it through the transcription site and extruding it to form a surrounding domain of recently transcribed decondensed chromatin. Although transcription sites have been identified in various cells, surrounding domains of recently transcribed decondensed chromatin have not. We report evidence that transcription sites associated with a tandem gene array in mouse cells are indeed surrounded by or adjacent to a domain of decondensed chromatin composed of sequences from the gene array. Formation of this decondensed domain requires transcription and topoisomerase IIα activity. The decondensed domain is enriched for the trimethyl H3K36 mark that is associated with recently transcribed chromatin in yeast and several mammalian systems. Consistent with this, chromatin immunoprecipitation demonstrates a comparable enrichment of this mark in transcribed sequences at the tandem gene array. These results provide new support for the pol II factory model, in which an immobilized polymerase molecule extrudes decondensed, transcribed sequences into its surroundings.
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spelling pubmed-20643572007-12-18 Organization of chromatin and histone modifications at a transcription site Müller, Waltraud G. Rieder, Dietmar Karpova, Tatiana S. John, Sam Trajanoski, Zlatko McNally, James G. J Cell Biol Research Articles According to the transcription factory model, localized transcription sites composed of immobilized polymerase molecules transcribe chromatin by reeling it through the transcription site and extruding it to form a surrounding domain of recently transcribed decondensed chromatin. Although transcription sites have been identified in various cells, surrounding domains of recently transcribed decondensed chromatin have not. We report evidence that transcription sites associated with a tandem gene array in mouse cells are indeed surrounded by or adjacent to a domain of decondensed chromatin composed of sequences from the gene array. Formation of this decondensed domain requires transcription and topoisomerase IIα activity. The decondensed domain is enriched for the trimethyl H3K36 mark that is associated with recently transcribed chromatin in yeast and several mammalian systems. Consistent with this, chromatin immunoprecipitation demonstrates a comparable enrichment of this mark in transcribed sequences at the tandem gene array. These results provide new support for the pol II factory model, in which an immobilized polymerase molecule extrudes decondensed, transcribed sequences into its surroundings. The Rockefeller University Press 2007-06-18 /pmc/articles/PMC2064357/ /pubmed/17576795 http://dx.doi.org/10.1083/jcb.200703157 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Müller, Waltraud G.
Rieder, Dietmar
Karpova, Tatiana S.
John, Sam
Trajanoski, Zlatko
McNally, James G.
Organization of chromatin and histone modifications at a transcription site
title Organization of chromatin and histone modifications at a transcription site
title_full Organization of chromatin and histone modifications at a transcription site
title_fullStr Organization of chromatin and histone modifications at a transcription site
title_full_unstemmed Organization of chromatin and histone modifications at a transcription site
title_short Organization of chromatin and histone modifications at a transcription site
title_sort organization of chromatin and histone modifications at a transcription site
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064357/
https://www.ncbi.nlm.nih.gov/pubmed/17576795
http://dx.doi.org/10.1083/jcb.200703157
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