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Organization of chromatin and histone modifications at a transcription site
According to the transcription factory model, localized transcription sites composed of immobilized polymerase molecules transcribe chromatin by reeling it through the transcription site and extruding it to form a surrounding domain of recently transcribed decondensed chromatin. Although transcripti...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064357/ https://www.ncbi.nlm.nih.gov/pubmed/17576795 http://dx.doi.org/10.1083/jcb.200703157 |
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author | Müller, Waltraud G. Rieder, Dietmar Karpova, Tatiana S. John, Sam Trajanoski, Zlatko McNally, James G. |
author_facet | Müller, Waltraud G. Rieder, Dietmar Karpova, Tatiana S. John, Sam Trajanoski, Zlatko McNally, James G. |
author_sort | Müller, Waltraud G. |
collection | PubMed |
description | According to the transcription factory model, localized transcription sites composed of immobilized polymerase molecules transcribe chromatin by reeling it through the transcription site and extruding it to form a surrounding domain of recently transcribed decondensed chromatin. Although transcription sites have been identified in various cells, surrounding domains of recently transcribed decondensed chromatin have not. We report evidence that transcription sites associated with a tandem gene array in mouse cells are indeed surrounded by or adjacent to a domain of decondensed chromatin composed of sequences from the gene array. Formation of this decondensed domain requires transcription and topoisomerase IIα activity. The decondensed domain is enriched for the trimethyl H3K36 mark that is associated with recently transcribed chromatin in yeast and several mammalian systems. Consistent with this, chromatin immunoprecipitation demonstrates a comparable enrichment of this mark in transcribed sequences at the tandem gene array. These results provide new support for the pol II factory model, in which an immobilized polymerase molecule extrudes decondensed, transcribed sequences into its surroundings. |
format | Text |
id | pubmed-2064357 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20643572007-12-18 Organization of chromatin and histone modifications at a transcription site Müller, Waltraud G. Rieder, Dietmar Karpova, Tatiana S. John, Sam Trajanoski, Zlatko McNally, James G. J Cell Biol Research Articles According to the transcription factory model, localized transcription sites composed of immobilized polymerase molecules transcribe chromatin by reeling it through the transcription site and extruding it to form a surrounding domain of recently transcribed decondensed chromatin. Although transcription sites have been identified in various cells, surrounding domains of recently transcribed decondensed chromatin have not. We report evidence that transcription sites associated with a tandem gene array in mouse cells are indeed surrounded by or adjacent to a domain of decondensed chromatin composed of sequences from the gene array. Formation of this decondensed domain requires transcription and topoisomerase IIα activity. The decondensed domain is enriched for the trimethyl H3K36 mark that is associated with recently transcribed chromatin in yeast and several mammalian systems. Consistent with this, chromatin immunoprecipitation demonstrates a comparable enrichment of this mark in transcribed sequences at the tandem gene array. These results provide new support for the pol II factory model, in which an immobilized polymerase molecule extrudes decondensed, transcribed sequences into its surroundings. The Rockefeller University Press 2007-06-18 /pmc/articles/PMC2064357/ /pubmed/17576795 http://dx.doi.org/10.1083/jcb.200703157 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Müller, Waltraud G. Rieder, Dietmar Karpova, Tatiana S. John, Sam Trajanoski, Zlatko McNally, James G. Organization of chromatin and histone modifications at a transcription site |
title | Organization of chromatin and histone modifications at a transcription site |
title_full | Organization of chromatin and histone modifications at a transcription site |
title_fullStr | Organization of chromatin and histone modifications at a transcription site |
title_full_unstemmed | Organization of chromatin and histone modifications at a transcription site |
title_short | Organization of chromatin and histone modifications at a transcription site |
title_sort | organization of chromatin and histone modifications at a transcription site |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064357/ https://www.ncbi.nlm.nih.gov/pubmed/17576795 http://dx.doi.org/10.1083/jcb.200703157 |
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