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Molecular characterization of centriole assembly in ciliated epithelial cells
Ciliated epithelial cells have the unique ability to generate hundreds of centrioles during differentiation. We used centrosomal proteins as molecular markers in cultured mouse tracheal epithelial cells to understand this process. Most centrosomal proteins were up-regulated early in ciliogenesis, in...
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Formato: | Texto |
Lenguaje: | English |
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The Rockefeller University Press
2007
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064416/ https://www.ncbi.nlm.nih.gov/pubmed/17606865 http://dx.doi.org/10.1083/jcb.200703064 |
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author | Vladar, Eszter K. Stearns, Tim |
author_facet | Vladar, Eszter K. Stearns, Tim |
author_sort | Vladar, Eszter K. |
collection | PubMed |
description | Ciliated epithelial cells have the unique ability to generate hundreds of centrioles during differentiation. We used centrosomal proteins as molecular markers in cultured mouse tracheal epithelial cells to understand this process. Most centrosomal proteins were up-regulated early in ciliogenesis, initially appearing in cytoplasmic foci and then incorporated into centrioles. Three candidate proteins were further characterized. The centrosomal component SAS-6 localized to basal bodies and the proximal region of the ciliary axoneme, and depletion of SAS-6 prevented centriole assembly. The intraflagellar transport component polaris localized to nascent centrioles before incorporation into cilia, and depletion of polaris blocked axoneme formation. The centriolar satellite component PCM-1 colocalized with centrosomal components in cytoplasmic granules surrounding nascent centrioles. Interfering with PCM-1 reduced the amount of centrosomal proteins at basal bodies but did not prevent centriole assembly. This system will help determine the mechanism of centriole formation in mammalian cells and how the limitation on centriole duplication is overcome in ciliated epithelial cells. |
format | Text |
id | pubmed-2064416 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20644162008-01-02 Molecular characterization of centriole assembly in ciliated epithelial cells Vladar, Eszter K. Stearns, Tim J Cell Biol Research Articles Ciliated epithelial cells have the unique ability to generate hundreds of centrioles during differentiation. We used centrosomal proteins as molecular markers in cultured mouse tracheal epithelial cells to understand this process. Most centrosomal proteins were up-regulated early in ciliogenesis, initially appearing in cytoplasmic foci and then incorporated into centrioles. Three candidate proteins were further characterized. The centrosomal component SAS-6 localized to basal bodies and the proximal region of the ciliary axoneme, and depletion of SAS-6 prevented centriole assembly. The intraflagellar transport component polaris localized to nascent centrioles before incorporation into cilia, and depletion of polaris blocked axoneme formation. The centriolar satellite component PCM-1 colocalized with centrosomal components in cytoplasmic granules surrounding nascent centrioles. Interfering with PCM-1 reduced the amount of centrosomal proteins at basal bodies but did not prevent centriole assembly. This system will help determine the mechanism of centriole formation in mammalian cells and how the limitation on centriole duplication is overcome in ciliated epithelial cells. The Rockefeller University Press 2007-07-02 /pmc/articles/PMC2064416/ /pubmed/17606865 http://dx.doi.org/10.1083/jcb.200703064 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Vladar, Eszter K. Stearns, Tim Molecular characterization of centriole assembly in ciliated epithelial cells |
title | Molecular characterization of centriole assembly in ciliated epithelial cells |
title_full | Molecular characterization of centriole assembly in ciliated epithelial cells |
title_fullStr | Molecular characterization of centriole assembly in ciliated epithelial cells |
title_full_unstemmed | Molecular characterization of centriole assembly in ciliated epithelial cells |
title_short | Molecular characterization of centriole assembly in ciliated epithelial cells |
title_sort | molecular characterization of centriole assembly in ciliated epithelial cells |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064416/ https://www.ncbi.nlm.nih.gov/pubmed/17606865 http://dx.doi.org/10.1083/jcb.200703064 |
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