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Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation
The Diaphanous-related formin Dia1 nucleates actin polymerization, thereby regulating cell shape and motility. Mechanisms that control the cellular location of Dia1 to spatially define actin polymerization are largely unknown. In this study, we identify the cytoskeletal scaffold protein IQGAP1 as a...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
The Rockefeller University Press
2007
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064439/ https://www.ncbi.nlm.nih.gov/pubmed/17620407 http://dx.doi.org/10.1083/jcb.200612071 |
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author | Brandt, Dominique T. Marion, Sabrina Griffiths, Gareth Watanabe, Takashi Kaibuchi, Kozo Grosse, Robert |
author_facet | Brandt, Dominique T. Marion, Sabrina Griffiths, Gareth Watanabe, Takashi Kaibuchi, Kozo Grosse, Robert |
author_sort | Brandt, Dominique T. |
collection | PubMed |
description | The Diaphanous-related formin Dia1 nucleates actin polymerization, thereby regulating cell shape and motility. Mechanisms that control the cellular location of Dia1 to spatially define actin polymerization are largely unknown. In this study, we identify the cytoskeletal scaffold protein IQGAP1 as a Dia1-binding protein that is necessary for its subcellular location. IQGAP1 interacts with Dia1 through a region within the Diaphanous inhibitory domain after the RhoA-mediated release of Dia1 autoinhibition. Both proteins colocalize at the front of migrating cells but also at the actin-rich phagocytic cup in macrophages. We show that IQGAP1 interaction with Dia1 is required for phagocytosis and phagocytic cup formation. Thus, we identify IQGAP1 as a novel component involved in the regulation of phagocytosis by mediating the localization of the actin filament nucleator Dia1. |
format | Text |
id | pubmed-2064439 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2007 |
publisher | The Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-20644392008-01-16 Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation Brandt, Dominique T. Marion, Sabrina Griffiths, Gareth Watanabe, Takashi Kaibuchi, Kozo Grosse, Robert J Cell Biol Research Articles The Diaphanous-related formin Dia1 nucleates actin polymerization, thereby regulating cell shape and motility. Mechanisms that control the cellular location of Dia1 to spatially define actin polymerization are largely unknown. In this study, we identify the cytoskeletal scaffold protein IQGAP1 as a Dia1-binding protein that is necessary for its subcellular location. IQGAP1 interacts with Dia1 through a region within the Diaphanous inhibitory domain after the RhoA-mediated release of Dia1 autoinhibition. Both proteins colocalize at the front of migrating cells but also at the actin-rich phagocytic cup in macrophages. We show that IQGAP1 interaction with Dia1 is required for phagocytosis and phagocytic cup formation. Thus, we identify IQGAP1 as a novel component involved in the regulation of phagocytosis by mediating the localization of the actin filament nucleator Dia1. The Rockefeller University Press 2007-07-16 /pmc/articles/PMC2064439/ /pubmed/17620407 http://dx.doi.org/10.1083/jcb.200612071 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Research Articles Brandt, Dominique T. Marion, Sabrina Griffiths, Gareth Watanabe, Takashi Kaibuchi, Kozo Grosse, Robert Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation |
title | Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation |
title_full | Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation |
title_fullStr | Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation |
title_full_unstemmed | Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation |
title_short | Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation |
title_sort | dia1 and iqgap1 interact in cell migration and phagocytic cup formation |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064439/ https://www.ncbi.nlm.nih.gov/pubmed/17620407 http://dx.doi.org/10.1083/jcb.200612071 |
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