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Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport

The phenylanine-glycine (FG)–rich regions of several nucleoporins both bind to nuclear transport receptors and collectively provide a diffusion barrier to the nuclear pores. However, the in vivo roles of FG nucleoporins in transport remain unclear. We have inactivated 30 putative nucleoporins in cul...

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Autores principales: Sabri, Nafiseh, Roth, Peggy, Xylourgidis, Nikos, Sadeghifar, Fatemeh, Adler, Jeremy, Samakovlis, Christos
Formato: Texto
Lenguaje:English
Publicado: Rockefeller University Press|1 2007
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064463/
https://www.ncbi.nlm.nih.gov/pubmed/17682050
http://dx.doi.org/10.1083/jcb.200612135
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author Sabri, Nafiseh
Roth, Peggy
Xylourgidis, Nikos
Sadeghifar, Fatemeh
Adler, Jeremy
Samakovlis, Christos
author_facet Sabri, Nafiseh
Roth, Peggy
Xylourgidis, Nikos
Sadeghifar, Fatemeh
Adler, Jeremy
Samakovlis, Christos
author_sort Sabri, Nafiseh
collection PubMed
description The phenylanine-glycine (FG)–rich regions of several nucleoporins both bind to nuclear transport receptors and collectively provide a diffusion barrier to the nuclear pores. However, the in vivo roles of FG nucleoporins in transport remain unclear. We have inactivated 30 putative nucleoporins in cultured Drosophila melanogaster S2 cells by RNA interference and analyzed the phenotypes on importin α/β−mediated import and CRM1-dependent protein export. The fly homologues of FG nucleoporins Nup358, Nup153, and Nup54 are selectively required for import. The FG repeats of Nup153 are necessary for its function in transport, whereas the remainder of the protein maintains pore integrity. Inactivation of the CRM1 cofactor RanBP3 decreased the nuclear accumulation of CRM1 and protein export. We report a surprisingly antagonistic relationship between RanBP3 and the Nup214 FG region in determining CRM1 localization and its function in protein export. Our data suggest that peripheral metazoan FG nucleoporins have distinct functions in nuclear protein transport events.
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spelling pubmed-20644632008-02-13 Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport Sabri, Nafiseh Roth, Peggy Xylourgidis, Nikos Sadeghifar, Fatemeh Adler, Jeremy Samakovlis, Christos J Cell Biol Research Articles The phenylanine-glycine (FG)–rich regions of several nucleoporins both bind to nuclear transport receptors and collectively provide a diffusion barrier to the nuclear pores. However, the in vivo roles of FG nucleoporins in transport remain unclear. We have inactivated 30 putative nucleoporins in cultured Drosophila melanogaster S2 cells by RNA interference and analyzed the phenotypes on importin α/β−mediated import and CRM1-dependent protein export. The fly homologues of FG nucleoporins Nup358, Nup153, and Nup54 are selectively required for import. The FG repeats of Nup153 are necessary for its function in transport, whereas the remainder of the protein maintains pore integrity. Inactivation of the CRM1 cofactor RanBP3 decreased the nuclear accumulation of CRM1 and protein export. We report a surprisingly antagonistic relationship between RanBP3 and the Nup214 FG region in determining CRM1 localization and its function in protein export. Our data suggest that peripheral metazoan FG nucleoporins have distinct functions in nuclear protein transport events. Rockefeller University Press|1 2007-08-13 /pmc/articles/PMC2064463/ /pubmed/17682050 http://dx.doi.org/10.1083/jcb.200612135 Text en Copyright © 2007, The Rockefeller University Press This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Research Articles
Sabri, Nafiseh
Roth, Peggy
Xylourgidis, Nikos
Sadeghifar, Fatemeh
Adler, Jeremy
Samakovlis, Christos
Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport
title Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport
title_full Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport
title_fullStr Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport
title_full_unstemmed Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport
title_short Distinct functions of the Drosophila Nup153 and Nup214 FG domains in nuclear protein transport
title_sort distinct functions of the drosophila nup153 and nup214 fg domains in nuclear protein transport
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2064463/
https://www.ncbi.nlm.nih.gov/pubmed/17682050
http://dx.doi.org/10.1083/jcb.200612135
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